Application of furanocoumarins in the preparation of antiallergic drugs

A technology of furanocoumarins and compounds, applied in the field of biomedicine, can solve problems such as painful life-threatening, hypersensitivity reactions, and elevated calcium ion concentration in mast cells, and achieve the effect of inhibiting hypersensitivity reactions

Active Publication Date: 2019-08-23
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Clinically, anaphylactoid reactions may occur when patients take the drug for the first time, which not only brings huge economic burden to patients, but also causes new pain and even life-threatening
Studies have found that the human MrgprX2 receptor (Mas-related G protein Euclidean receptor X2) can mediate anaphylactoid reactions. When drugs stimulate the human MrgprX2 receptor, it can increase the calcium ion concentration of mast cells and trigger a degranulation reaction. leading to allergic reactions

Method used

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  • Application of furanocoumarins in the preparation of antiallergic drugs
  • Application of furanocoumarins in the preparation of antiallergic drugs
  • Application of furanocoumarins in the preparation of antiallergic drugs

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Experimental materials

[0025] Instrument: Automatic microplate reader was purchased from Bio-Rad (California, USA).

[0026] Cell line: KU812 was cultured in IMEM medium with 10% serum, plus 1:100 penicillin-streptomycin. The medium was half-replaced with fresh medium every other day to maintain the cells at 2 × 10 6 density of cells per milliliter.

[0027] Main reagent: TM buffer solution (composition (g / L): 6.954 NaCl, 0.353 KCl, 2.383 HEPES, 0.162 KH 2 PO 4 , 0.282 CaCl 2 , 0.143 MgSO 4 , 0.991 glucose, 1 bovine serum albumin. Dimethyl sulfoxide was purchased from Guangzhou Jinhuada Chemical Reagent Co., Ltd., and C48 / 80 was purchased from Sigma-Aldrich (St.Louis, MO, USA). The imperatorin, isoimperatorin, and pectinoids were all purchased from Chengdu Pufeid Biotechnology Co., Ltd. (Chengdu, China). Triton X-100 was purchased from Kehao Bioengineering Company (Xi'an, China), D-glucose Tianjin Kemiou Chemical Reagent Company (Tianjin, China), bovine ser...

Embodiment 2

[0052] 1. Experimental materials

[0053] Instrument: LC-2010AHT (SHIMAZU), self-made column packing machine.

[0054] Cell line: MrgprX2-HEK293 cells were cultured in DMEM medium with 10% fetal bovine serum, plus 1:100 penicillin-streptomycin in 5% CO 2 , in an environment of 37°C. The medium was replaced with fresh medium every other day to keep the cells healthy.

[0055] Main reagents: DMEM medium and fetal bovine serum were purchased from Hyclone (Logan, Utah, USA), and macroporous silica gel was purchased from Qingdao Meigao Group Co., Ltd. (Qingdao, China).

[0056] 2. Experimental method

[0057] 2.1 Preparation of MrgprX2-HEK293 cell membrane stationary phase

[0058] Take the above-mentioned MrgprX2-HEK293 cells (about 7×10 6 cells) suspension, centrifuge at 1000rpm, 4°C for 5min, discard the supernatant, and take the bottom cells; wash the centrifuged cells twice with 5mL saline, and then add Tris-HCl 5mL to the obtained cells Ultrasound for 30 min in an ice b...

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Abstract

The invention provides application of furocoumarins compound in preparing anti-allergic medicine and belongs to the technical field of biological medicine. The application disclosed by the invention proves that the furocoumarins compound can effectively antagonize beta-hexosaminidase release caused by C48 / 80 of human mast cell KU812 for the first time and further can inhibit anaphylactoid reaction; furthermore, the application also proves that an action receptor of the furocoumarins compound is an MrgprX2 receptor for the first time; when the furocoumarins compound in preparing anti-allergic medicine is prepared into anti-allergic preparation, anti-allergic medicine types can be enriched, and a brand-new choice and a brand-new strategy are provided for anti-allergic treatment.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and particularly relates to the application of furanocoumarin compounds in the preparation of antiallergic drugs. Background technique [0002] Drug-like allergic reactions are caused by the direct stimulation of mast cells or basophils by drugs, resulting in the degranulation of mast cells and the release of active transmitters such as β-hexosaminidase and histamine. Clinically, anaphylactoid reactions may occur when patients take medicine for the first time, which not only brings huge economic burden to patients, but also causes new pain and even life-threatening. Studies have found that the human MrgprX2 receptor (Mas-related G protein Euclidean receptor X2) can mediate anaphylactoid reactions. When drugs stimulate the human MrgprX2 receptor, it can increase the calcium ion concentration of mast cells and trigger a degranulation reaction. This can lead to allergic reactions. Clinically u...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/37A61P37/08
Inventor 贺浪冲林园园张涛贺怀贞王珏
Owner XI AN JIAOTONG UNIV
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