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Detection method of medicine injection anaphylactic and anaphylactoid reactions and new application of adopted dye

A technology of allergic reaction, detection method, applied in the field of cell biology and pharmacy

Active Publication Date: 2015-10-28
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Anaphylaxis includes the sensitization stage and the antigen re-stimulation process, but the anaphylactoid reaction is triggered directly after the allergen-like stimulation without the sensitization process; both can cause mast cells / basophils to degranulate and release allergic mediators, thereby causing Local / systemic allergy symptoms

Method used

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  • Detection method of medicine injection anaphylactic and anaphylactoid reactions and new application of adopted dye
  • Detection method of medicine injection anaphylactic and anaphylactoid reactions and new application of adopted dye
  • Detection method of medicine injection anaphylactic and anaphylactoid reactions and new application of adopted dye

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Detection of hypersensitivity reactions to Danhong injection, a traditional Chinese medicine injection

[0037] Danhong injection is a commonly used traditional Chinese medicine for the clinical treatment of cardiovascular and cerebrovascular diseases. The composition is diverse and it has its own color, so choosing Danhong Injection as the research object has certain representative significance. In order to examine the reliability of the method, 30 batches of Danhong Injection with clinical reports of allergic symptoms were collected as research samples.

[0038] Main Drugs and Reagents

[0039] Danhong injection (DHI, Danhong injection) is a product of Heze Buchang Pharmaceutical Co., Ltd. MEM basal medium (Cat No. 41500034) and fetal bovine serum are products of Gibco, USA. When culturing cells, 10% fetal bovine serum and 1% penicillin+streptomycin were added to the MEM basal medium. Compound48 / 80 (Sigma, Cat No. 2313) was dissolved in MEM phenol red-free...

Embodiment 2

[0069] Example 2: Western medicine injection puerarin injection class allergic reaction detection

[0070] Puerarin injection is mainly used for auxiliary treatment of coronary heart disease, angina pectoris and other diseases. From January 1, 2003 to June 30, 2005, a total of 1006 cases of puerarin adverse reactions were reported by the National Adverse Reaction Testing Center. From 2004 to now, there have been clinical reports on allergic reactions caused by puerarin injection almost every year, among which reports of anaphylactic shock are common. It is clearly stated in the instructions that those who are allergic to puerarin injection or those with allergic constitution are forbidden to use puerarin injection. The high-content detection method using puerarin injection as the detection object can better reflect the feasibility of the method.

[0071] Main Drugs and Reagents

[0072] Puerarin injection (PI, Puerarin injection) is a product of Jinan Limin Pharmaceutical C...

Embodiment 3

[0093] Example 3: Detection of anaphylactoid reactions caused by the allergen-like chlorogenic acid

[0094] Chlorogenic acid has pharmacological effects such as cardiovascular protection, anti-oxidation, anti-cancer, and antibacterial. Many injections that cause allergic reactions, such as Shuanghuanglian injection, geniposide Yinzhihuang injection, Qingkailing injection, etc., contain chlorogenic acid, and allergies are related to chlorogenic acid. In many current studies on allergic reactions, chlorogenic acid is also used as a positive control group, which is a type of allergen known to cause cell degranulation.

[0095] Main Drugs and Reagents

[0096] Chlorogenic acid (Chl, Chlorogenic acid) standard was purchased from Chengdu Master Biotechnology Co., Ltd., and prepared as a 100 μm stock solution. MEM basal medium (Cat No. 41500034) and fetal bovine serum are products of Gibco, USA. When culturing cells, 10% fetal bovine serum and 1% penicillin+streptomycin were added...

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PUM

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Abstract

The invention provides a detection method of medicine injection anaphylactic and anaphylactoid reactions and the new application of adopted dye. Cytomembrane fluorescent dye capable of specifically staining cytomembranes and entering cells through endocytosis is adopted for marking the vesica circulating phenomenon on a cell degranulation module, analysis is carried out based on iconography information, a high content system is used as a detection means, intracellular fluorescence enhancement caused by medicine injections is detected on the high content system to reflect the degranulation condition of cells, and the probability that the anaphylactic and anaphylactoid reactions are caused by the medicine injections is predicted by calculating the cell degranulation positive rate through a fluorescence intensity conversion calculation method. The adopted dye comprises FM4-64, FM1-43, AM1-43 or AM4-66. The detection method is easy to operate, the result is objective, and important application value is achieved.

Description

technical field [0001] The invention relates to the fields of cell biology and pharmacy, in particular to a method for detecting hypersensitivity reactions of drug injections and a new application of the dye used. Background technique [0002] Drug injections play an important role in the treatment of critical illnesses, but adverse reactions have always been the main problem plaguing its clinical application. Among the adverse reactions of drug injections, allergic / anaphylactoid reactions have always ranked first. Therefore, rapid detection of allergic / anaphylactoid reactions of drug injections is of great significance for the safe production and clinical application of injections. [0003] The traditional evaluation methods for allergic / anaphylactoid reactions of drug injections include whole animal testing methods and cell biochemical media testing methods. The overall animal detection method uses animals such as mice, guinea pigs, dogs, and cynomolgus monkeys to observe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C12Q1/02
Inventor 徐砚通高秀梅吴红华王化龙李振虎
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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