The present invention relates to the technical field of feed processing and particularly relates to a whey protein selenium-enriched biological feed and an application thereof. According to the research of the applicant, a lactic acid bacterium source selenium is prepared by chelating lactic acid bacteria with selenium element, can maintain a relatively high activity in animal gastric juice of high acidity, and can protect intestinal tracts of animals. At the same time, milk discharged from animal mammary glands is the highest part of selenium conversion in the whole animal body, therefore, the present application studies to develop the selenium-enriched feed. The feed consists of whey protein powder, bean powder, chequer-shaped indocalamus leaves, safflower carthamus, an enzyme preparation and a lactic acid bacterium source organic selenium solution, wherein the bean powder is composed of soybean powder, black bean powder and broad bean powder; and the enzyme preparation is composed of complex protease and carboxypeptidase. The whey protein selenium-enriched biological feed is reasonable in components, has a good synergistic effect, can effectively protect intestinal and mammary gland functions of animals, and improves conversion rate of selenium in mammals.