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96 results about "WHOLE ANIMAL" patented technology
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Protein fragment complementation assays in whole animals applications to drug efficacy, ADME, cancer biology, immunology, infectious disease and gene therapy
InactiveUS7306914B2Literature is vastSimplifying assayUltrasonic/sonic/infrasonic diagnosticsAntibody mimetics/scaffoldsWHOLE ANIMALFluorescence
The instant invention describes a method for detecting protein-protein interactions in living organisms and / or cells, said method comprising: (a) synthesizing probe protein fragments from a protein which enables fluorescent or luminescent detection by dissecting the gene coding for the fluorescent or luminescent protein into a least two fragments; (2) constructing fusion proteins consisting of the probe protein fragments linked to protein domains that are to be tested for interactions; (3) coexpressing the fusion proteins; and (d) detecting reconstitution of the fluorescence or luminescence signal.
Owner:ODYSSEY THERA INC
Application of arctigenin and its derivative in medicament preparation
InactiveCN102342929APromote phosphorylationEasy to manufactureOrganic active ingredientsAntinoxious agentsWHOLE ANIMALMedicine
The invention relates to application of arctigenin as shown in general formula (1) and its derivative in preparing medicaments for improving body tolerance (resisting fatigue), and application of pharmaceutical compositions containing arctigenin and its derivative for improving body tolerance (resisting fatigue). The arctigenin and its derivative play a promoting role in AMPK phosphorylation. Whole animal tests show that compounds of the invention can enhance fatigue tolerance and can be used for anti-fatigue treatment.
Owner:SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
Lactobacillus plantarum UCN-11 strain, and composition and application thereof
ActiveCN104611275APreventing or preventing gasPrevent diarrheaAntibacterial agentsBacteriaWHOLE ANIMALAntibiotic Y
The invention belongs to the fields of microbes, food preservation and animal feed, and particularly relates to a Lactobacillus plantarum UCN-11 strain, and a composition and application thereof. The new strain has the functions of food preservation and animal health care, and can ensure the shelf life of food and high-efficiency healthy cultivation of animals. The collection number of the new strain at China General Microbiological Culture Collection Center is CGMCC No.9480. The strain, fermentation nutrient material, fermentation liquid or thallus obtained by centrifugating the fermentation nutrient material, and the dried substance of the fermentation liquid or thallus are effective constituents for preparing food. The preparation process does not need to add any chemical preservative or perform irradiation treatment, maintains the original freshness of the meat food and obviously enhances the quality. The shelf life can exceed 6 months. The strain can remove the peculiar smell in the meat product and improve the flavor. The food can be eaten for a long time, and can increase the quantity of probiotics in the human body. No antibiotics are added in the whole animal feeding process, thereby obviously promoting healthy growth of animals, lowering the cost and enhancing the economic benefit.
Owner:成都然新生物科技有限公司
Two ScFv (Single Chain Variable Fragment ) antibodies, encoding genes and application thereof for preparing preparation for treating or preventing infectious bursal disease of chicken
InactiveCN103483449AStrong specificityGood treatment effectBacteriaImmunoglobulins against virusesAnimal virusDisease
The invention discloses two types of ScFv (Single Chain Variable Fragment) antibodies, encoding genes of the antibodies and applications of the antibodies for preparing the preparations for treating or preventing infectious bursal disease of chicken. Two single-chain antibodies (namely, the ScFv antibodies) are provided; and the ScFv antibodies can be specially bound with the protein 2 (VP2) (Virus Protein 2) in an IBDV (Infectious Bursal Disease Virus) structure and a plurality of IBDV strains to block the cytopathic effect (CPE) of the chicken embryo fibroblast by the IBDV so as to protect the young chicken infected with IBDV. The immune serum and egg yolk antibody are tedious in preparation, high in production cost, unstable in effect, difficult to control industrialized production quality, capable of causing horizontal disease spread and the like in a use process; the ScFv antibodies disclosed by the invention can overcome the above disadvantages and have the advantages of strong specificity, good treatment effect, controllable industrialized production quality, capability of preventing the horizontal disease spread caused by the egg yolk antibody and the like; and therefore, by virtue of the ScFv antibodies, a new situation is opened up in the IBDV prevention and treatment history, and even in the entire prevention and treatment history for animal virus diseases.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
Environment-friendly extraction method for animal oil and fat
InactiveCN108559621AEasy to operate and controlNice appearanceFatty-oils/fats refiningFatty-oils/fats productionOil and greaseWHOLE ANIMAL
The invention relates to an environment-friendly extraction method for animal oil and fat. The method includes animal oil and fat crushing, smelting, oil residue separation, meat residue squeezing, filtering, peculiar smell deodorization and oil purification. The animal oil smelting and extraction form assembly line work, the whole animal oil extraction process has the characteristics of simple and convenient operation control and high work efficiency, and the produced animal oil and fat have few impurities, good oil color and high quality.
Owner:新乡市红阳油脂工程技术有限公司
Methods for screening antimicrobial and antiviral compounds and uses thereof
ActiveUS20100216852A1Extend your lifeReduce and eliminate incidenceAntibacterial agentsBiocideWHOLE ANIMALScreening method
The invention features compounds that have antibacterial activity, their use for the treatment of bacterial infections, screening methods that use whole animals or plant seedlings to identify compounds that inhibit a pathogen in the animal or plant, and screening methods to identify compounds that increase the lifespan of an organism.
Owner:THE GENERAL HOSPITAL CORP +1
Animal pill magnet having single polarity
An improved animal pill or cow magnet that is inserted into the stomach of a bovine animal to attract and hold tramp metal ingested by the animal so as to prevent the adverse condition commonly referred to as “hardware disease.” The disclosed device has at least a pair of NdFeB magnets separated by a carbon steel section within the stainless steel outer casing. The resulting magnetic field for the overall animal pill magnet has a single polarity, which by design can be either N or S.
Owner:ANIMAL SUPPLIES INT
Self-adaption data analysis method for small-animal brain functional magenetic resonance imaging
ActiveCN107689057AReduce digging depthReduce analysisImage enhancementImage analysisHead movementsWHOLE ANIMAL
The invention discloses a self-adaption data analysis method for small-animal brain functional magenetic resonance imaging. The method includes the following steps that whole-brain scanning images ofall test animals are obtained multiple times, head movement correction is carried out, and whole-brain average images of all the animals are obtained; a whole-brain image of some animal serves as a reference image, image registration and image averaging are iteratively carried out, and a self-adaption brain template is obtained; in-brain pixels of the self-adaption brain template are extracted ina multichannel gaussian filter mode to serve as original in-brain-pixel mask images; in-brain-pixel mask images matched with self-adaption brain template space are obtained; all test individual brainimages and the self-adaption brain template are subjected to space standardization, and skull peeling is carried out; time sequences of all interest areas of all the test animals are obtained; throughthe pearson correlation algorithm, correlation indexes between all the interest areas are obtained, and a whole-animal network of the test animals is obtained. The technical scheme is wide in adaptation and high in efficiency, and is not influenced by artificial subjective factors.
Owner:INST OF HIGH ENERGY PHYSICS CHINESE ACADEMY OF SCI
Protein fragment complementation assays in whole animals applications to drug efficacy, ADME, cancer biology, immunology, infectious disease and gene therapy
InactiveUS20050079547A1Literature is vastSimplifying assayUltrasonic/sonic/infrasonic diagnosticsAntibody mimetics/scaffoldsWHOLE ANIMALFluorescence
The instant invention describes a method for detecting protein-protein interactions in living organisms and / or cells, said method comprising: (a) synthesizing probe protein fragments from a protein which enables fluorescent or luminescent detection by dissecting the gene coding for the fluorescent or luminescent protein into a least two fragments; (2) constructing fusion proteins consisting of the probe protein fragments linked to protein domains that are to be tested for interactions; (3) coexpressing the fusion proteins; and (d) detecting reconstitution of the fluorescence or luminescence signal.
Owner:ODYSSEY THERA INC
Trough capable of stirring feed for animal husbandry
The invention discloses a trough capable of stirring feed for animal husbandry. The trough comprises a base and a processing compartment. The front and back sides of an inner cavity of the processingcompartment are vertically provided with a first sliding rod and a second sliding rod respectively. A threaded rod is arranged at the upper half section of the first sliding rod, a feed stirring framecomprises a lifting frame, a threaded sleeve and a sliding sleeve are installed at the left and right ends of the lifting frame respectively, the threaded sleeve is installed on the threaded rod, thesliding sleeve is installed on the second sliding rod, a stirring shaft is installed in the middle of the lifting frame, a stirring frame is installed on the stirring shaft, and the stirring frame islocated over the trough. Feed processing is combined with feeding operation, the feed processing operation efficiency is greatly improved on the basis that the requirement for animal husbandry supplyis met, and therefore the whole animal husbandry feeding technological procedures are reduced.
Owner:HEFEI TOTEM LONG MACHINERY DESIGN CO LTD
Reaction-based fluorescent probe for selective detection of carbon monoxide using metal-mediated carbonylation
ActiveUS20150057185A1Microbiological testing/measurementPalladium organic compoundsWHOLE ANIMALFluorescence
Carbon monoxide (CO) is a member of the gasotransmitter family that includes NO and H2S and is implicated in a variety of pathological and physiological conditions. Whereas exogenous therapeutic additions of CO to tissues and whole animals have been well studied, the real-time spatial and temporal tracking of CO at the cellular level remains an open challenge. We now report a new type of turn-on fluorescent probe for selective CO detection by exploiting palladium-mediated carbonylation reactivity. The compounds of the invention are capable of detecting CO both in aqueous buffer and in live cells with high selectivity over a range of biologically relevant reactive small molecules, providing a potentially powerful approach for interrogating its chemistry in biological systems.
Owner:RGT UNIV OF CALIFORNIA
3-(2-carboxylbenzoyl)-oleanolic acid, pharmaceutical composition of same and application of same in treating diabetes and/or obesity
InactiveCN101525366AEasy to synthesizeEasy to manufactureOrganic active ingredientsMetabolism disorderDiseaseWHOLE ANIMAL
Owner:SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
Multibar apparatus and method for electrically stimulating a carcass
InactiveUS7892076B2Avoid problemsReduce the amount requiredElectric-based meat tenderisingPoultry processingWHOLE ANIMALAnatomy
Owner:JBS USA FOOD CO +1
Whey protein selenium-enriched biological feed and application thereof
InactiveCN109430526APromote lactationHas the effect of invigorating bloodFood processingAnimal feeding stuffBiotechnologyWHOLE ANIMAL
The present invention relates to the technical field of feed processing and particularly relates to a whey protein selenium-enriched biological feed and an application thereof. According to the research of the applicant, a lactic acid bacterium source selenium is prepared by chelating lactic acid bacteria with selenium element, can maintain a relatively high activity in animal gastric juice of high acidity, and can protect intestinal tracts of animals. At the same time, milk discharged from animal mammary glands is the highest part of selenium conversion in the whole animal body, therefore, the present application studies to develop the selenium-enriched feed. The feed consists of whey protein powder, bean powder, chequer-shaped indocalamus leaves, safflower carthamus, an enzyme preparation and a lactic acid bacterium source organic selenium solution, wherein the bean powder is composed of soybean powder, black bean powder and broad bean powder; and the enzyme preparation is composed of complex protease and carboxypeptidase. The whey protein selenium-enriched biological feed is reasonable in components, has a good synergistic effect, can effectively protect intestinal and mammary gland functions of animals, and improves conversion rate of selenium in mammals.
Owner:广西壮佳霖农业投资有限公司
Fast detection method of poultry and animal epiornitic and drug residues self hemagglutination
InactiveCN1920571AStrong specificityImprove accuracyMaterial analysisWHOLE ANIMALSingle-Chain Antibodies
The invention relates to a method for detecting causal agent and left drug in whole animal blood, wherein it uses gene engineering method or chemical couple method to couple the causal agent antigen, causal agent antibody, drug antibody with single chain antibody or single colon antibody of red blood cell to obtain one dual-function agent which can combine red blood cell and combine causal agent antigen, causal agent antibody, and left drug antibody. The inventive agent can use the red blood cell of animal as instructor to detect the causal agent and left drug of animal.
Owner:河南省生物工程技术研究中心
Use of cortex eucommiae total polyoses in the preparing of the medicine for preventing and treating systematic lupus erythematosus
The invention belong to Chinese traditional medicine field, relates to the application of eucommia polysaccharide for preparing medicine preventing systematic Cazenave's lupus. The invention separates polysaccharide extract from eucommia, the average productivity is 2.2%, and the polysaccharide content is more than 68%. It is showed by whole animal model test that the protective effect for hyper reaction and kidney injury for mice by said polysaccharide is distinctive, and it is proven that polysaccharide can prevent Cazenave's lupus syndrome. The eucommia polysaccharide can also be used to produce medicine preventing and treating self-immune disease.
Owner:FUDAN UNIV
Source tracing method and system for grazing animal growth process
PendingCN108537561ARealize whole-process monitoringReal-time acquisitionCo-operative working arrangementsCommerceWHOLE ANIMALAcquisition time
The invention discloses a source tracing method and system for a grazing animal growth process, and relates to the field of source tracing of the animal growth process. The method comprises the following steps: an animal information acquisition device integrating functions of locating information acquisition, environmental information acquisition and animal sign information acquisition is mountedon a collar of each animal; the animal information acquisition device stores acquired animal motion trail information, environmental information, sign information and an identifier correspondingly according to acquisition time; stored data is transmitted to a data storage and backup server remotely; an analysis and judgment server comprehensively judges basic information, the environmental information and the sign information of each animal with a first preset method for determining the health condition of the animal, and analyzes the motion trail information with a second preset method for determining an animal feeding manner. Whole-process monitoring of the whole animal growth process and automatic analysis of data can be realized, so that accuracy for determining the health condition ofthe animals and the feeding manner is improved.
Owner:NINGXIA UNIVERSITY
Application of preclinical pharmacokinetic key technology and research system in cefoperazone sodium and sulbactam sodium
The invention provides application of a preclinical pharmacokinetic key technology and research system in cefoperazone sodium and sulbactam sodium, comprising the steps of (1) after the cefoperazone sodium and sulbactam sodium with a certain concentration is administrated to an experimental animal for a certain time, collecting one or more biological samples in blood, urine and faeces; (2) treating the biological samples obtained in step (1) by adopting liquid-liquid extraction, albumen precipitation, and solid phase extraction technologies to obtain corresponding solutions; (3) analyzing the prepared solutions in step (2) by adopting an LC-MS(liquid chromatography-mass spectrography) and an LC-MS / MS((liquid chromatography-tandem mass spectrometry). According to the application provided by the invention, the Caco-2 cell can also be adopted to detect the membrane permeability of the medicine and cell absorptive capacity; or the medicine with a certain concentration treats primary culture cerebral microvascular endothelial cells to detect the blood-brain barrier permeability of the medicine; or by adopting a whole animal, S9, human intestinal microsome and monoclonal purified enzyme, in vitro metabolism stability of the medicine is detected and a metabolite is identified.
Owner:刘晓东 +1
Systems and methods for improved rendering
ActiveCN104370996AFatty-oils/fats refiningPeptide preparation methodsWHOLE ANIMALRendering (animal products)
Systems and methods for improved rendering of animal products may utilize a multistep process including at least a heating step through use of a steam-jacketed tube system and a pressing step to squeeze out the oils. Grinding and pre-breaking may first be used to break the fat / whole animal into pieces. The ground material may then be transported and heated in one or more steam-jacketed tubes to create a slurry. A press may then be employed to press out the oil and water from the slurry. Residual solids may be removed from the oil and water through a decanting step. Water and extremely fine materials may then be removed from the oil through a centrifuge step, and the oil may be transferred to one or more storage tanks. Following the pressing step, the protein that remains may be subjected to high pressure to squeeze out any residual fat and then may be transferred to a hammer mill to prepare into a powdered protein or other desired protein material that may be stored for later use.
Owner:詹姆斯 K 日特尼克
Method for peptide and polypeptide purification and differential analysis
A method for peptide and polypeptide purification that uses a derivatized solid support to capture all of the polypeptides and peptides present in a solution and in a subsequent step quantitatively release all of the polypeptides and peptides bound to the solid support is disclosed. This method provides for quantitative recovery and permits further analysis by any of a variety of analytical methods, including sequencing, chromatography, mass spectrometry and biological assay. The method may further be used for screening peptides and polypeptides that bind to organic molecules such as small molecule drugs. The method does not irreversibly add any unwanted label moieties or otherwise alter the peptides during the release process that could interfere with subsequent processing or analysis steps, in particular, biological assay to assess function in vivo or in vitro in cell-based, whole animal or human testing.
Owner:KRAKOVER JONATHAN +1
Automatic barbecue oven
InactiveCN105125093AStructural combination design is scientific and reasonableMeasures uniqueRoasting apparatusWHOLE ANIMALMotor drive
The invention discloses an automatic barbecue oven which specifically comprises a base frame, a motor, rotating systems, a barbecue frame, a bearing pedestal and a chain. The low-power motor drives the five rotating systems to rotate. Each rotating system rotates and bears one or more kinds of barbecue food to be rotated for barbecuing. The barbecue food is rotationally barbecued in a fuel box and evenly heated without being burnt. The phenomenon that people near the barbecue oven constantly keep an eye on and overturn the barbecue food and are attacked by heat is avoided, and the people keeping an eye on the barbecue food are prevented from inhaling carbon dioxide released in the charcoal combustion process and smoke released by barbecue food. The whole barbecue process is free and easy, and the barbecue quality of the barbecue good is greatly improved. When the automatic barbecue oven is in use, small barbecue food can be barbecued, large barbecue food can also be barbecued, such as a whole animal, the integrality of the animal is kept, and the commercial value of the barbecue food is greatly improved.
Owner:许帼桉
Reaction-based fluorescent probe for selective detection of carbon monoxide using metal-mediated carbonylation
Carbon monoxide (CO) is a member of the gasotransmitter family that includes NO and H2S and is implicated in a variety of pathological and physiological conditions. Whereas exogenous therapeutic additions of CO to tissues and whole animals have been well studied, the real-time spatial and temporal tracking of CO at the cellular level remains an open challenge. We now report a new type of turn-on fluorescent probe for selective CO detection by exploiting palladium-mediated carbonylation reactivity. The compounds of the invention are capable of detecting CO both in aqueous buffer and in live cells with high selectivity over a range of biologically relevant reactive small molecules, providing a potentially powerful approach for interrogating its chemistry in biological systems.
Owner:RGT UNIV OF CALIFORNIA
New application of benzatropine and pharmaceutically acceptable salt thereof
InactiveCN105853420AEnhanced inhibitory effectGood water solubilityAntineoplastic agentsHeterocyclic compound active ingredientsWHOLE ANIMALMda mb 231
The invention relates to a new application of benzatropine and pharmaceutically acceptable salt thereof. The benzatropine and pharmaceutically acceptable salt thereof have a very strong inhibitory effect on the breast cancer cell lines MCF-7, 4T1-luc2, MDA-MB-231 and SKBR3; and the whole animal experiment also has an obvious effect and proves that the benzatropine and pharmaceutically acceptable salt thereof can be used for preparing medicines for resisting breast tumor and inhibiting breast tumor metastasis.
Owner:SUN YAT SEN UNIV
Liver tissue cell removal solution
InactiveCN103083726AImprove decellularization efficiencyEasy to configure and useProsthesisWHOLE ANIMALRecombinant tissue plasminogen activator
The invention discloses a liver tissue cell removal solution. The novel cell removal solution prepared from two components is introduced into a whole animal cell removal technology. The cell removal solution is prepared from 0.01 to 0.05 percent of recombinant tissue plasminogen activator (rt-PA) and 0.5 to 2 percent of tween-80 by using sterile normal saline. By using the strong thrombolytic effect of the rt-PA and the solubilization and red blood cell destruction effects of the tween-80, the liver tissue cell removal solution is relatively easy to prepare and implement, high in cell removal efficiency and low in requirement for warm ischemia time of the liver, and greatly improves the potential probability of a cell removal liver scaffold in clinical application.
Owner:昆明市第一人民医院
New application of conjugated linoleic acid to promotion of fracture healing
InactiveCN105193779AHigh activityEnhanced osteogenic activityOrganic active ingredientsSkeletal disorderWHOLE ANIMALSerum alkaline phosphatase
The invention discloses new application of a conjugated linoleic acid to promotion of fracture healing. Relevant experiment research on whole animal and molecular cell level shows that the conjugated linoleic acid can further increase fracture healing strength by improving the quality of a rat fracture healing callus so as to achieve the fracture healing promotion. As proved by experiment results, the conjugated linoleic acid can promote fracture healing and enhance in-organism osteoblastic activity and improve ALP (serum alkaline phosphatase) activity. The invention hints that the conjugated linoleic acid has a prospect for developing a fracture healing promotion drug.
Owner:SUZHOU UNIV
Glue from slaughterhouse animal blood
Disclosed is a process for making an animal blood adhesive and the resulting adhesive composition. The adhesive is made by adding to fresh, whole animal blood, without dewatering the blood, an anticoagulant and a preservative. Lime is then added to the anticoagulated, preserved blood, and the pH is adjusted to between about 9 and about 11. A curing agent (such as potassium silicate, sodium silicate and aluminum dihydrogen phosphate) and ammonia are then added to the mixture to yield the animal blood adhesive. The adhesive can be made from any animal blood, such as bovine, porcine, or avian blood. The resulting adhesive is useful for adhering wood-based products, such as plywood and particle board.
Owner:WISCONSIN ALUMNI RES FOUND
Use of bupleurum root total polyoses in the preparing of medicine for preventing and treating acute respiratory distress syndrome
The invention belong to Chinese traditional medicine field, relates to the application of bupleurum root polysaccharide for preparing medicine preventing and treating acute respiratory distress syndrome. The invention separates polysaccharide extract from bupleurum root, the average productivity is 3.32%, and the polysaccharide content is more than 68.2%-78.6%. It is showed by whole animal model test that the protective effect for lung injury caused by acute respiratory distress syndrome is distinctive, and it is proven that polysaccharide can prevent acute respiratory distress syndrome. The bupleurum root polysaccharide can also be used to produce medicine preventing and treating fowl influenza.
Owner:FUDAN UNIV
Application of preclinical pharmacokinetics key technology and research system in methionine and vitamin b1
InactiveCN105572247AImprove bindingPromotes the detoxification processComponent separationBiological testingWHOLE ANIMALMetabolite
Owner:刘晓东 +1
Automatic contour recognition method for tomographic images
PendingCN112381084ARealize automatic identificationImprove recognition accuracyImage enhancementImage analysisWHOLE ANIMALValue set
The invention discloses an automatic contour recognition method for a tomographic image. The method comprises the following steps: after an acquired tomographic image is de-noised, determining a boundary gray value T of a foreground and a background in a single image, and respectively correcting a foreground image gray value set and a background image gray value set by utilizing the boundary grayvalue T, a selected foreground image gray value and a selected background image gray value; and obtaining first foreground image feature data P2 and background image feature data P1, and inputting theforeground image feature data P2 and the background image feature data P1 through an onecut algorithm to obtain an identified second foreground image P3. According to the method, automatic identification of each image contour in multiple tissue slice images is realized, the method is particularly used for automatic processing of animal tissue slice image data with about thousands of layers, automatic contour information identification and extraction of the whole animal tissue slice tomographic image data are realized, and any identification parameters do not need to be modified manually.
Owner:WUHAN OE BIO CO LTD
Animal target image extraction method and system
ActiveCN108038864AImprove detection accuracyEasy to controlImage analysisWHOLE ANIMALImage extraction
The invention provides an animal target image extraction method and system. A fuzzy set theory membership function combining brightness information, brightness level information and neighborhood gradient information is designed; through the designed fuzzy set theory membership function and a brightness histogram curve, a shadow region and other regions in an animal image are preliminarily distinguished; shadow regions in other regions are accurately detected by adopting an Otsu method; all the shadow regions in the whole animal image are detected out; the shadow regions are removed to obtain the animal image; the detection precision of the shadow regions and a target region in the animal image is high; main external factors influencing the image are well adjusted and controlled; relativelyhigh shadow detection precision and robust performance are achieved; and a foundation is laid for work of target tracking, feature extraction and the like.
Owner:CHINA AGRI UNIV
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