Use of bupleurum root total polyoses in the preparing of medicine for preventing and treating acute respiratory distress syndrome

A technology of acute respiratory distress and total polysaccharide, applied in the field of traditional Chinese medicine, can solve problems such as the efficacy of total polysaccharide in the prevention and treatment of acute respiratory distress syndrome and its drugs that have not been reported yet.

Active Publication Date: 2008-01-02
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Taking a comprehensive view of the research at home and abroad, there is no report on the efficacy of Bupleurum polysaccharides in the prevention and treatment of acute respiratory distress syndrome and its medicinal properties.

Method used

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  • Use of bupleurum root total polyoses in the preparing of medicine for preventing and treating acute respiratory distress syndrome
  • Use of bupleurum root total polyoses in the preparing of medicine for preventing and treating acute respiratory distress syndrome
  • Use of bupleurum root total polyoses in the preparing of medicine for preventing and treating acute respiratory distress syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Take 100g of Bupleurum herb, extract by cold immersion in 95% ethanol, put the dregs in a ventilated place at room temperature to dry, then extract with hot water for 3 times, filter, combine the extracts, concentrate, centrifuge, and the supernatant with S3 Chloroacetic acid removes free protein, centrifuges, and dialyzes the supernatant with water for 3 days, concentrates the dialysate to a small volume, adds ethanol to an alcohol content of 80%, centrifuges, precipitates and freeze-dries to obtain the total polysaccharide, and the product yield is more than 3%. The content exceeds 65%. Table 3 shows the yield and content of total polysaccharides in Bupleurum chinensis.

[0044] The Bupleurum medicinal material can be selected from Bupleurum chinense DC, Bupleurum scorzonerifolium Willd or Bupleurum smithii Wolff.

[0045] table 3

[0046] batch number

Embodiment 2

[0048] Bupleurum chinense DC total polysaccharides (about 3.0mg.mL -1) was double-diluted with barbitol buffer solution (BBS) to obtain eight concentrations of solutions, 0.2ml of each concentration sample was added to 0.2ml of complement (1:32 dilution of guinea pig serum), 0.1ml of 2% sheep Red blood cells (sheep red blood cell, SRBC) and 0.1ml 1:1000 hemolysin (anti-SRBC serum), put each tube in a 37°C water bath for 30 minutes, then place it in a low-temperature high-speed centrifuge at 2500G and 4°C, and take the supernatant of each tube after 20 minutes 0.25mL was measured at 405nm in a microplate reader for absorbance. Then just mix 0.2ml of the sample solution of each concentration with 0.4ml of BBS, put it into a low-temperature high-speed centrifuge and centrifuge at 2500G, 4°C for 10min, take 0.25mL of the supernatant from each tube and measure the absorbance at 405nm in a microplate reader as the natural color value of the sample. Then, the absorbance of each diff...

Embodiment 3

[0050] The total polysaccharides of Bupleurum scorzonerifolium Willd (about 3.0mg.mL -1 ) was double-diluted with barbitol buffer solution (BBS) to obtain eight concentrations of solutions, 0.2ml of each concentration sample was added to 0.2ml of complement (1:32 dilution of guinea pig serum), 0.1ml of 2% sheep Red blood cells (sheep red blood cell, SRBC) and 0.1ml 1:1000 hemolysin (anti-SRBC serum), put each tube in a 37°C water bath for 30 minutes, place it in a low-temperature high-speed centrifuge at 2500G, and centrifuge at 4°C. Clear 0.25mL and measure the absorbance at 405nm in a microplate reader. Then just mix 0.2ml of the sample solution of each concentration with 0.4ml of BBS, put it into a low-temperature high-speed centrifuge and centrifuge at 2500G, 4°C for 10min, take 0.25mL of the supernatant from each tube and measure the absorbance at 405nm in a microplate reader as the natural color value of the sample. Then, the absorbance of each different concentration s...

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Abstract

The invention belong to Chinese traditional medicine field, relates to the application of bupleurum root polysaccharide for preparing medicine preventing and treating acute respiratory distress syndrome. The invention separates polysaccharide extract from bupleurum root, the average productivity is 3.32%, and the polysaccharide content is more than 68.2%-78.6%. It is showed by whole animal model test that the protective effect for lung injury caused by acute respiratory distress syndrome is distinctive, and it is proven that polysaccharide can prevent acute respiratory distress syndrome. The bupleurum root polysaccharide can also be used to produce medicine preventing and treating fowl influenza.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and relates to a new medical application of total polysaccharide of Bupleurum bupleuri. It specifically relates to the use of Bupleurum total polysaccharides in the preparation of medicines for preventing and treating acute respiratory distress syndrome. Background technique: [0002] Acute respiratory distress syndrome (ARDS) refers to acute and progressive hypoxic respiratory failure caused by various pathogenic factors in and outside the lungs other than cardiogenic, and its clinical characteristics are acute respiratory distress and refractory Acute hypoxemia is one of the more common clinical critical and severe diseases with a high fatality rate. Davidson TA (Davidson TA, Caldwell ES, et al. JAMA, 1999, 281: 354.) reported that the mortality rate of ARDS was as high as 30%-40% in the past ten years. Patients with severe atypical pneumonia (SARS) may also experience respiratory ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/233A61P11/00A61P31/14A61P31/16A61P43/00A61K125/00
Inventor 陈道峰徐晗章蕴毅周捷张建文
Owner FUDAN UNIV
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