Application of preclinical pharmacokinetics key technology and research system in methionine and vitamin b1

Abstract

The invention provides application of a preclinical pharmacokinetics key technology and a research system in methionine and vitamin b1. The application comprises the following steps of (1) collecting one or several biological sample(s) of blood, urine and excrement after giving the methionine and vitamin b1 in a certain concentration to an experimental animal for a certain time; (2) treating the biological samples obtained in step (1) by adopting a liquid-liquid extraction technology, a protein precipitation technology and a solid-phase extraction technology, and preparing corresponding solutions; (3) analyzing the solutions prepared in step (2) by adopting LC-MS (Liquid Chromatography-Mass Spectrometry) and LC-MS / MS. According to the application provided by the invention, the membrane permeability and the cell absorbing ability of the methionine and vitamin b1 can also be detected by adopting Caco-2 cells, or the detection of in-vitro metabolism stability of the methionine and vitamin b1 and the identification of a metabolite can be realized by adopting a whole animal, S9, human liver microsomes and monoclonal purification enzyme.

Description

technical field [0001] The invention relates to the application of key preclinical pharmacokinetics technology and research system in methionine vitamin b1. Background technique [0002] The liver is an important organ of the human body, and my country is a country with a large number of liver diseases. Methionine vitamin b1 is mainly used to improve liver function, and has obvious curative effect on liver diseases, such as acute and chronic hepatitis, liver cirrhosis, especially fatty liver, and can improve intrahepatic cholestasis; it can be used for alcohol, barbiturate There is adjuvant treatment for poisoning by class and sulfa drugs. [0003] Pharmacokinetics is a discipline that studies the absorption, distribution, metabolism and excretion process and laws of drugs in organisms, and corresponds to pharmacodynamics, which studies the biological activities of drugs on organisms. Pharmacokinetics focuses on the study of the body's disposal of drugs, but with the devel...

AI Technical Summary

Problems solved by technology

With the transformation of my country's new drug R&D model from "imitation-based" to "combination of imitation and innovation" and "innovation-based", the original pharmacokinetic research and evaluation system in my country can no longer meet the needs of the development and transformation of new drug R&D models. Cannot give full play to the guiding role of pharmacokinetic research in the structural design and optimization of lead compounds, the design and modification of new dosage forms, the prediction of drug interactions, and the clinical safety and rational use of drugs, etc.