Fast detection method of poultry and animal epiornitic and drug residues self hemagglutination

A technology of erythrocyte agglutination, livestock and poultry, which is applied in the field of detection of erythrocyte agglutination, can solve problems such as difficult to meet the requirements of rapid detection at the grassroots level, difficulty in grassroots units, and affecting food safety, so as to reduce feeding costs, achieve significant social benefits, and guarantee The effect of food safety

Inactive Publication Date: 2007-02-28
河南省生物工程技术研究中心
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Problems solved by technology

However, due to the experimental conditions and prices, these reagents (boxes) are difficult to meet the requirements of rapid on-site detection at the grassroots level
[0007] At present, there are two major problems in my country's food safety: first, microbial contamination is still the most important factor affecting food hygiene and safety in my country, food contamination caused by pathogenic microorganisms has seriously damaged people's health, and the control of pathogenic microb

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  • Fast detection method of poultry and animal epiornitic and drug residues self hemagglutination
  • Fast detection method of poultry and animal epiornitic and drug residues self hemagglutination
  • Fast detection method of poultry and animal epiornitic and drug residues self hemagglutination

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Embodiment Construction

[0030] 1. The preparation methods of erythrocyte antibody, pathogen antibody, and drug small molecule antibody are basically similar. Now, the preparation of erythrocyte antibody is taken as an example to illustrate the implementation plan:

[0031] Red blood cells were used as immune antigens to immunize BALB / c mice. The spleen lymphocytes of the immunized mice were fused with myeloma cells, and then monoclonals that could secrete erythrocyte monoclonal antibodies were obtained by limiting dilution. Specific steps are as follows:

[0032] 1. Take the whole blood of healthy animals, add anticoagulant trisodium citrate, centrifuge at 3000rpm at low speed, extract red blood cells and wash them three times with normal saline, add normal saline containing antibiotics and dilute to 2×10 per ml 7 Cells, the initial immunization adopts subcutaneous multi-point injection, 0.1ml / point, a total of four points. Two weeks later, the second immunization was performed with the same dosage as...

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Abstract

The invention relates to a method for detecting causal agent and left drug in whole animal blood, wherein it uses gene engineering method or chemical couple method to couple the causal agent antigen, causal agent antibody, drug antibody with single chain antibody or single colon antibody of red blood cell to obtain one dual-function agent which can combine red blood cell and combine causal agent antigen, causal agent antibody, and left drug antibody. The inventive agent can use the red blood cell of animal as instructor to detect the causal agent and left drug of animal.

Description

Technical field [0001] The invention relates to a detection method for pathogens, pathogen antigens, pathogen antibodies, and residual drugs in the whole blood of livestock and poultry, and belongs to the autoerythrocyte agglutination detection of livestock and poultry disease pathogens, pathogen antigens, pathogen antibodies, and residual drugs in the field of disease diagnosis and food safety detection Law. technical background [0002] Erythrocyte agglutination test, also known as hemagglutination test, is an immunoassay technique using red blood cells as a reaction indicator. This long-standing immunological method is widely used in primary hospitals because of its simple operation and no need for equipment. Its sensitivity problem is gradually eliminated. In 1988, Australian scholars coupled the Fab' fragment of human erythrocyte monoclonal antibody with HIV synthetic peptide antigen, obtained a bifunctional chemical cross-linked product, and established a detection me...

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Application Information

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IPC IPC(8): G01N33/577G01N33/569G01N33/531
Inventor 李晨阳李玉林陈小科
Owner 河南省生物工程技术研究中心
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