Fast detection method of poultry and animal epiornitic and drug residues self hemagglutination

Abstract

The invention relates to a method for detecting causal agent and left drug in whole animal blood, wherein it uses gene engineering method or chemical couple method to couple the causal agent antigen, causal agent antibody, drug antibody with single chain antibody or single colon antibody of red blood cell to obtain one dual-function agent which can combine red blood cell and combine causal agent antigen, causal agent antibody, and left drug antibody. The inventive agent can use the red blood cell of animal as instructor to detect the causal agent and left drug of animal.

Description

Technical field [0001] The invention relates to a detection method for pathogens, pathogen antigens, pathogen antibodies, and residual drugs in the whole blood of livestock and poultry, and belongs to the autoerythrocyte agglutination detection of livestock and poultry disease pathogens, pathogen antigens, pathogen antibodies, and residual drugs in the field of disease diagnosis and food safety detection Law. technical background [0002] Erythrocyte agglutination test, also known as hemagglutination test, is an immunoassay technique using red blood cells as a reaction indicator. This long-standing immunological method is widely used in primary hospitals because of its simple operation and no need for equipment. Its sensitivity problem is gradually eliminated. In 1988, Australian scholars coupled the Fab' fragment of human erythrocyte monoclonal antibody with HIV synthetic peptide antigen, obtained a bifunctional chemical cross-linked product, and established a detection me...

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Problems solved by technology

However, due to the experimental conditions and prices, these reagents (boxes) are difficult to meet the requirements of rapid on-site detection at the grassroots level

[0007] At present, there are two major problems in my country's food safety: first, microbial contamination is still the most important factor affecting food hygiene and safety in my country, food contamination caused by pathogenic microorganisms has seriously damaged people's health, and the control of pathogenic microbial contamination is still It is the main content to solve the problem of food pollution at present; the second is that source pollution such as planting and breeding seriously affects food safety

However, at present, the detection of these targets is mainly concentrated in qualified laboratories, which require high-pressure liquid chromatography, PCR instrument, microplate reader and other equipment, and personnel also need to be trained. It is difficult for grassroots units to do this.

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