Method for detecting anaphylactoid reaction caused by chlorogenic acids

A technology for allergic reactions and acids, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of short half-life, lack of a series of biological information, complicated operation, etc., to achieve convenient operation, saving detection time, saving The effect of human and material resources

Inactive Publication Date: 2013-08-21
BIOMEDICAL ANALYSIS CENT OF ACADEMY OF MILITARY MEDICAL SCI +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a main medicinal ingredient, chlorogenic acid is also a suspicious sensitizer, but it has not yet been determined whether this type of ingredient is an allergen, and its objective truth needs to be clarified urgently! At present, there is a lack of sufficiently reliable animal models for the detection of anaphylactoid reactions, and it is difficult to establish corresponding evaluation methods
The newly developed mouse auricle blue dye test and

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting anaphylactoid reaction caused by chlorogenic acids
  • Method for detecting anaphylactoid reaction caused by chlorogenic acids
  • Method for detecting anaphylactoid reaction caused by chlorogenic acids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: The method of the present invention for detecting anaphylactoid reactions caused by chlorogenic acid components. The chlorogenic acid components include chlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, and cryptochlorogenic acid or neochlorogenic acid, the method of the present invention can simultaneously monitor different concentrations and various chlorogenic acid monomers causing anaphylactoid reactions, and anaphylactoid reactions are chemically or pharmacologically mediated without the participation of the immune system. Yes, a clinical reaction with symptoms similar to anaphylaxis is shown at the first administration. Example 1 cites a method for detecting anaphylactoid reactions caused by chlorogenic acid solutions. The real-time cell analyzers used in this example are all DP-type real-time cell analyzers.

[0047] 1. Instruments used in the test: RTCA DP (German Roche Company); E-Plate 16 detection plate (6x6 ...

Embodiment 2

[0057] Embodiment two: what this embodiment enumerates is to monitor the growth index value of RBL-2H3 cell in the isochlorogenic acid A solution under different concentrations, the test instrument used is the same as embodiment one, isochlorogenic acid A reference substance (purity greater than 98 %), purchased from Chengdu Master Biotechnology Co., Ltd.

[0058] The steps of the method for detecting anaphylactoid reaction caused by isochlorogenic acid A solution at different concentrations include the following steps, including the following steps, (1) preparing RBL-2H3 cell suspension and isochlorogenic acid A solution of different concentrations respectively; ( 2) Use the real-time cell analyzer to monitor the cell growth index in the RBL-2H3 suspension with different cell concentrations, and determine the RBL-2H3 cell suspension used when the real-time cell analyzer detects the allergic reaction caused by the isochlorogenic acid A solution The optimal cell concentration a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for detecting anaphylactoid reaction caused by chlorogenic acids. The method comprises the following steps of (1) respectively preparing RBL-2H3 cell suspension and chlorogenic acid solution which are different in concentration; (2) determining the optimal cell concentration of the RBL-2H3 cell suspension and the optimal time point of addition of the chlorogenic acid solution when a real-time cell analyzer detects the anaphylactoid reaction caused by chlorogenic acids; and (3) detecting the RBL-2H3 cell growth index values before and after addition of the chlorogenic acid solution by utilizing a real-time cell analyzer. The method has the advantages that growth, extension, morphologic change and death physiological states of the RBL-2H3 cell before and after the chlorogenic acid solution is added can be reflected in real time; and the concentration range of the solution of the chlorogenic acids causing the anaphylactoid reaction can be obtained.

Description

technical field [0001] The invention belongs to the technical field of drug detection, in particular to a method for detecting anaphylactoid reactions caused by chlorogenic acid components. Background technique [0002] Chlorogenic acid is a phenylpropanoid compound produced by plants through the shikimic acid pathway during aerobic respiration. It is an important biologically active substance with antibacterial, antiviral, exciting central nervous system and scavenging free radicals effect. Chinese medicine injections containing chlorogenic acid components, such as Shuanghuanglian injection and Qingkailing injection, account for about 22% of the number of listed Chinese medicine injections, and their clinical curative effect is definite, but adverse reactions occur from time to time (mainly allergic reactions, Among them, class anaphylaxis accounts for about 70%-80%). As a main medicinal ingredient, chlorogenic acid is also a suspicious sensitizer, but it has not yet been...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/02
Inventor 鄢丹马丽娜张乐乐韩玉梅张毅肖小河
Owner BIOMEDICAL ANALYSIS CENT OF ACADEMY OF MILITARY MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap