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Method for detecting induced anaphylactoid reaction of medicines and application thereof

A detection method and anaphylaxis technology, which is applied in the field of detection of drug anaphylaxis, can solve the problems of affecting the detection sensitivity of anaphylaxis and interfering with the detection of anaphylaxis biomarkers, and achieves objective detection indicators, low interference, and The effect of the simplicity of the test method

Inactive Publication Date: 2015-03-18
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the model was applied, it was found that the Evans blue dye solution had a significant inhibitory effect on mast cell degranulation induced by some allergic drugs, which seriously affected the detection sensitivity of allergic reactions. At the same time, due to the dye solution injected into the body, Significantly interfered with detection of anaphylactoid biomarkers in blood and tissue

Method used

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  • Method for detecting induced anaphylactoid reaction of medicines and application thereof
  • Method for detecting induced anaphylactoid reaction of medicines and application thereof
  • Method for detecting induced anaphylactoid reaction of medicines and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Detection Principle and Correlation Between Systemic Symptoms and Blood Biomarker Levels

[0053] 1. Experimental materials

[0054] 1.1 Experimental animals

[0055] ICR mice, clean grade, male and female, 18-22g, purchased from Shanghai Xipuer-Bikay Experimental Animal Co., Ltd., certificate number: SCXK (Shanghai) 2008-0016, the above-mentioned animals are all in the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine Raised in SPF breeding room, free to drink water and ingest food during the experiment, and the indoor temperature was 20-22°C.

[0056] 1.2 Test substance

[0057] C48 / 80 (product of Sigma Company, batch number: 039K4023); Tween80 (V / V) (product of Sigma Company, batch number: BCBF4913V).

[0058] 1.3 Main reagents

[0059] Histamine ELISA kit (Demeditec product, batch number: 11411); IgE ELISA kit (ICL product, batch number: E-90E11Q1); β-hexosaminidase (Sigma company product, batch number: N9376); trypsin (...

Embodiment 2

[0160] Comparison of Example 2 and Mouse Ear Vascular Permeability Test Model in Detecting Anaphylaxis Sensitivity

[0161] 1. Experimental materials

[0162] 1.1 Experimental animals

[0163] ICR mice, clean grade, male and female, 18-22g, purchased from Shanghai Sipro-Bikay Experimental Animal Co., Ltd., certificate number: SCXK (Shanghai) 2008-0016, the above-mentioned animals are all in the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine Raised in SPF breeding room, free to drink water and ingest food during the experiment, and the indoor temperature was 20-22°C.

[0164] 1.21.2 Test substances, main reagents and preparation methods

[0165] The source, batch number and preparation method of C48 / 80 and Tween80, the source and batch number of the histamine ELISA kit are the same as those under item 1.2 in Example 1.

[0166] 1.3 Main instruments

[0167] Same as item 1.3 of the experimental material part in Example 1.

[0168] 2. Exper...

Embodiment 1

[0184] Comparative Example 1 Table 10, the ratio of anaphylaxis-like symptoms and blood biomarker levels observed by the test method of the present invention to the selected auricle blue stain rate, blue stain area score and auricle Yin Indicators such as the amount of exudation of Vance blue are more sensitive and have higher sensitivity. Comparing the two, the test method of the present invention is 17 times and 2 times more sensitive to the allergy-positive drugs C48 / 80 and Tween80 than the mouse ear blood vessel permeability test method. Even in the mouse ear vascular permeability test method, the blood histamine detection in the test method of the present invention is also adopted, and the sensitivity of the test method of the present invention to C48 / 80 is also 8 times higher than that of the mouse ear vascular permeability test method.

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Abstract

The invention relates to the technical field of medical evaluation and detection, and in particular relates to a method for detecting induced anaphylactoid reaction of intravenous injection medicines. The method comprises the following steps: observing anaphylactoid reaction symptoms of experimental animals after being dosed in a certain time by supplying different doses of tested medicines to intravenous injection of the experimental animals, and judging the grade of anaphylactoid reaction strength of intravenous injection administration of the tested medicines according to scale scores of the anaphylactoid reaction symptoms. According to the method disclosed by the invention, by adopting the anaphylactoid reaction symptoms of the animals after being dosed as 'gold standards', the whole process of dynamic reaction of the occurrence of clinical systemic anaphylactoid reaction can be relatively well simulated, so that the method can be applied to the detection and determination of anaphylactoid reaction of the intravenous injection medicines, also can be applied to the screening of anti-anaphylactoid medicines and the research of systemic anaphylactoid pathogenesis and medicine action mechanism, and has the advantages of simplicity and convenience in operation, short experimental period, good detection reproducibility, clinically-close results and the like.

Description

technical field [0001] The invention relates to the technical field of medical evaluation and detection, in particular to a method for detecting the anaphylaxis of drugs and related applications. Background technique [0002] Compared with other types of hypersensitivity, the concept of hypersensitivity-like reaction was put forward later, and its position in anaphylaxis is not clear at present. subclass of . The pathogenic mechanism of the drug in vivo includes the following process: after the drug enters the body, it activates effector cells-tissue mast cells and blood basophils, and the effector cells degranulate to release allergic active mediators such as histamine, which act on the target organs of the body to cause allergic symptoms. [0003] After activation, mast cells can release a variety of active substances, which act on a wide range of target organs in the body, causing pathological changes, such as vasodilation, bronchoconstriction, mucus secretion, nerve sti...

Claims

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Application Information

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IPC IPC(8): A61K49/00
Inventor 谢家骏张立波易娟娟康黎静赵琳
Owner SHANGHAI UNIV OF T C M
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