96 results about "Biofortification" patented technology

The invention relates to a method for treating total nitrogen of nitrogen-containing chemical wastewater. The method comprises preaeration tank treatment and two stages of A/O (anoxic/oxic) treatment. The wastewater treatment is carried out through a preaeration tank, a first-stage anaerobic tank, a first-stage aerobic tank, a second-stage anaerobic tank, a second-stage aerobic tank and a sedimentation tank, wherein denitrifier agent can be added in the preaeration tank, the first-stage aerobic tank and the second-stage aerobic tank for biofortification. The method has simple technological process, can reduce the influence of organic load on the subsequent biochemical systems by using the preaeration tank at the previous stage, improves the wastewater treatment ability, shortens the technological process and reduces the operation cost. By using two stages of A/O process to treat the coal chemical industry wastewater, the removing efficiency of the total nitrogen can be effectively improved. The method provided by the invention is applicable to treating coking wastewater or non-degradable nitrogen-containing wastewater such as pharmacy wastewater, monosodium glutamate wastewater and fermenting wastewater, as well as domestic wastewater and municipal wastewater.

The invention relates to a combined anoxic/aerobic enhanced biological activated carbon dynamic membrane nitrogen and phosphorus removal process. Enhanced biological activated carbon is cultured by using a microorganism inoculation and acclimatization method; and after domestic sewage is treated, effluent is filtered by a dynamic membrane which is pre-coated with the enhanced biological activated carbon. Powdered activated carbon is added to maintain the high sludge concentration in a reactor. The high sludge concentration can reinforce endogenous denitrification; the problem of nitrogen and phosphorus removal in a treatment process is solved by using a biological phosphorus removal process of the aerobic phosphorus uptake of phosphorus-accumulating bacteria synthetically; the solid-liquid separation of mixed liquid is realized by using the high-efficiency entrapment capability of the dynamic membrane, so that the effluent is filtered. The reactor used by the invention has the advantages of compact structure, simple construction, high contamination removal efficiency and convenient operation, management and maintenance. In the process, advanced nitrogen and phosphorus removal treatment can be performed under the condition of not adding extra carbon sources, and the effluence can meet the national recycled water standard. Dominant bacteria such as loss-easy nitrifying bacteria, denitrifying bacteria, phosphorus-accumulating bacteria and the like are grown in a way that the bacteria are attached to the surface of the powdered activated carbon. The enhanced biological activated carbon dynamic membrane has the advantages of large flux, short hydraulic retention time and capability of improving treatment efficiency.

The invention discloses a bio-augmentation treatment sludge in-situ reduction method, which is characterized in that: on the basis of a traditional active sludge biochemical treatment system, functional microbial inoculum is additionally added to enhance organic matter degradation and a metazoan is additionally added to enhance predation so as to optimize a biotic community structure, so the sludge in-situ reduction can be realized during the sewage treatment process, and the sludge reduction effect can reach 60 percent to 100 percent. The functional microbial inoculum is a mixed flora which is specially used for the sludge reduction and is obtained through directed selection domestication culture and comprises five types of special bacterial strain selected from soil and active sludge. The metazoan is a vermes-typed micro metazoan which is selected in a laboratory and is used for the bio-augmentation predation of an active sludge system through the propagation. Through the bio-augmentation treatment method, the sludge in-situ reduction can be realized under the situation that the normal running of a sewage plant is not influenced, and the method has the characteristics of less investment, low running expense, fast action, stable running and the like. The method has general adaptability to ordinary sewage biochemical treatment systems.

The invention discloses denitrifying phosphorus removal bacteria bacillus cereus H-hrb01, and belongs to the technical fields of biological engineering and environmental engineering, wherein bacillus cereus H-hrb01 belongs to bacillus genus by identification and is conserved in the general microbiology centre of the China committee for culture collection of microorganisms, the conservation date is March 27th 2012, and the conservation number is CGMCC NO.5939; bacillus cereus H-hrb01 can take various organic matters as carbon sources, has good denitrifying and phosphorus-removing efficiency, and can be used for sewage biological denitrification and dephosphorization process; bacillus cereus H-hrb01 is activated and cultivated, and fed into artificial wastewater to mix and then introduced into an SBR (styrene butadiene rubber) biofortification device, and each effluent index of the reactor is superior to that of a contrast system which is not fed with the bacterial strain. The removal rates of the COD (chemical oxygen demand), ammonia nitrogen and dissoluble phosphorus in the sewage are respectively up to 93.90%, 97.70% and 96.25%.

The invention discloses a polyculture enrichment screening method for ocean heterotrophic denitrifying bacteria. With the method, the ocean heterotrophic denitrifying bacteria can be enriched in an early-stage autotrophic mode and a later-stage heterotrophic mode, and the bacteria with heterotrophic nitrification capability and heterotrophic nitrification and aerobic denitrification capability can be guaranteed to propagate. The polyculture enrichment screening method comprises the following steps: firstly, separating a selective culture medium; then, detecting and separating the ammonia monooxygenase gene and the nitrite oxidoreductase gene of a bacterial strain; finally, in a heterotrophic culture medium, determining whether a bacterial strain with a target gene is expressed or not by a nitrogen balance test. The autotrophic mode and the heterotrophic mode are alternately used for domestication and, enrichment as well as separation to finally obtain the screened bacterial strain which is determined as the heterotrophic nitrification bacterial strain or the heterotrophic nitrification and aerobic denitrification bacterial strain. The polyculture enrichment of heterotrophic nitrification and aerobic denitrification bacteria is not only the breakthrough of the bacteria enrichment theory, the bacterial strain is immobilized with a biofortification method to have a great practical application meaning for the biological denitrification of ocean cycling breeding wastewater, and the method is suitable for treating wastewater in the nitrification unit for the factory high-density breeding.

The invention discloses a method for treating soil or oil sludge severely contaminated by petroleum based on combination of sodium persulfate compound oxidation and microbial flora biological enhancement. Contaminated soil or oil sludge is oxidized first with a sodium persulfate compound as the oxidizing agent in the presence of a surfactant, and then a mixed flora of bacillus sp. and acremonium sp. Y0997 or phanerochaete sp. F0996 is added twice continuously, so that intensified combined treatment of soil or oil sludge is achieved through intensified combination of chemical oxidization and fungus-bacterium flora biological sequences. In this way, the degrading efficiency of soil or oil sludge severely contaminated by petroleum can be improved remarkably, and repair period can be shortened greatly.

The present invention relates to a coupling water supply purifying method of biological reinforced powder active carbon and diatomite, which comprises the following steps: firstly, powder active carbon and diatomite are continuously fed into raw water which needs to be purified quantitatively to be stirred to mix through mechanism to enable the powder active carbon and the diatomite to suspend in water to absorb organic matters in the raw water; then, biological powder active carbon and diatomite particles coated with microorganism are formed by a labor reinforced method; and organic pollutants in the raw water is further degraded by a microorganism function. Compared with the prior art, on the basis of the coupling water supply purifying technique of biological reinforced powder active carbon and diatomite, the present invention has three functions of absorption, biological degradation and dynamic film separation, and can further reduce the content of pollutants in the raw water, in particular to aim to the problems of high raw-water permanganate index, ammonian and organic pollutants, and the like. Thus, drinking water after being purified satisfies every index in the new sanitary standard for life drinking water GB 5749-2006 issued by China.

The invention relates to the field of sewage treatment, and particularly relates to a technology for ABS high difficult chemical wastewater. According to the technology, a combined process of 'coagulating sedimentation + parallel-serial biological enhancement processing system + dynamic membrane bioreactor' is adopted to treat ABS production wastewater. A conventional coagulant is added into a coagulation sedimentation tank to mainly remove suspended matters, colloid particles and phosphor from intaken water. The parallel-serial biological enhancement processing system is developed from a conventional A2O process. In the system, an aerobic tank and an anaerobic tank are connected in parallel and then connected with an anoxic tank in series; the aerobic tank is provided with an independent sludge return-flow system; the anaerobic tank and the anoxic tank share another sludge return-flow system for denitrifying phosphorus removal. A cheap porous medium is employed as a support filter base material by the dynamic membrane bioreactor; both powder activated carbon and activated sludge are added in the reactor; and deep treatment of the wastewater is realized by forming a cake layer through suction filtration. The process is novel in technology and has the advantages of simple process flow, high processing efficiency and low operation cost.

The invention relates to a treatment method which is suitable for papermaking black liquor (in particular to alkaline pulping papermaking black liquor which takes wheat straw hood as raw material). The treatment method comprises the following steps of: firstly performing standing treatment on the fresh black liquor, adding a high-efficient alkaline separation agent into the black liquor after treatment, separating out lignin, and further performing biological strengthening technical treatment on filtrate. According to the process disclosed by the invention, after the combined treatment of thestanding treatment, an alkaline separation method and a biological strengthening system is performed on the black liquor with initial COD (chemical oxygen demand) of 83,000mg/l, pH of 13.1 and chromaticity of 225,000CU, the COD and chromaticity of outlet water are respectively reduced to below 15% and 7% of the original black liquor (namely the COD total removal rate and chromaticity total removal rate of the whole system are about 85% and 93% respectively), the outlet water can be mixed with a quite large quantity of middle-stage wastewater in a papermaking factory, and the treatment can be performed according to the existing comparatively mature middle-stage wastewater treatment method. The process has the characteristics of low investment and operation cost and capability of recycling the lignin, and is suitable for wheat straw pulp papermaking factories above 5000 tons.

The invention discloses a livestock and poultry breeding wastewater treatment method. The high-concentration livestock and poultry breeding wastewater is economically and efficiently treated by utilizing a packaged process formed by an adjusting pretreatment tank, a primary sedimentation tank, an ammonia stripping tower, an anaerobic hydrolysis tank, an anaerobic ammonia oxidation tank, a biological enhancement tank, an aerobic activated sludge tank, an anoxic tank, a secondary sedimentation tank, a flocculation precipitation tank and a dehydrator, thus ensuring smooth and stable treatment of the livestock and poultry wastewater, relieving the economic burden of farmers and simultaneously solving the pollution problem.

Treatment of high-concentration organic waste water for grease process and discharge in refinery is carried out by applying saccharomycete for one-level SBR, aerating, biologically degrading for high-concentration CODCr and oil, mixing it with partial crude waste water, passing through active sludge second-level SBR, drastic treating, making outlet CODCr<100 mg/L and discharging up to standard. It's cheap, stable and automatic, has excellent load resisting and microbial-inhibiting performances. It can be used for middle-medium grease refinery plant.

The invention relates to an enhanced biochemical treatment method for high ammonia-nitrogen wastewater, which belongs to the technical field of sewage treatment. Anaerobic-hypoxia-aerobic combined process is adopted in the method, reactors are biological filter reactors, macroporous polyurethane-based carriers are filled in the reactors, high-efficiency composite microorganisms are fed into a biological membrane acclimation and cultured in biological filters, and high ammonia-nitrogen wastewater is treated in an enhanced manner by immobilization technology. The enhanced biochemical treatment has the advantages that biological filter process and enhanced biological treatment process are effectively combined by the immobilization technology, nitrogen removal efficiency is high, high-efficiency bacteria fixedly grow in the carriers, a biological inhibiting effect of high-content ammonia and nitrogen upon organisms is relieved, carrying capacity for change of load of pollutant of incoming water is high, system impact resistance is realized, and the enhanced biochemical treatment method is a high-efficiency combined biological treatment method.

The invention relates to the field of environmental engineering sewage treatment, in particular to an advanced treatment process for chemical industry park wastewater. Directed at the secondary biochemical effluent from chemical industry park sewage plants, the process utilizes electric flocculation and air flotation for pretreatment to separate suspended matters, the separated wastewater is subjected to ozone catalyzed oxidation decomposition and destruction of heterocyclic hardly degradable organic matters in wastewater, and the wastewater chroma is lowered, then the wastewater enters a biological aerated filter system for biological enhanced treatment, and the treated effluent enters a clean water tank, thus finally realizing discharging meeting the first level standard A. The advancedtreatment process is economical and feasible, and provides a reliable, efficient and stable combination technology for advanced treatment of chemical industry park wastewater.

The invention discloses a bio-augmentation technology for fermentation process of solid-state brewing vinegar, belonging to the technical field of biological Engineering. The operation procedures of the method comprise: (1) determining functional microorganisms for brewing vinegar: analyzing the structure of the microflora in vinegar fermented grains with molecular ecology technology such as PCR-DGGE, determining the functional microorganisms in the process of brewing vinegar by combining the key physicochemical indexes related to the quality and functionality of vinegar; (2) pure culture of the functional microorganisms for brewing vinegar: using microorganism pure culture technique to get the functional microorganisms for brewing vinegar from the vinegar fermented grains; and (3) bio-augmentation in the vinegar fermentation process: selecting the functional microorganisms or a combination thereof, and carrying out bio-augmentation in the solid-state fermentation stage of vinegar. The method has the advantages of shortening brewing cycle of vinegar, improving and controlling the flavor, quality and functionality of vinegar products, improving the utilization rate of raw material and the like.

The invention discloses a facility for treating pollutants in rainwater runoff. The facility comprises a water inlet pipe, a covering layer, a vegetation layer, an adsorption layer, an iron-improved sand layer, a submersion layer, a gravel layer, a water outlet pipe and a breathable facility, wherein the rainwater runoff enters the facility from the water inlet pipe and flows out from the water outlet pipe after being treated by units; the breathable facility is arranged in the middle of the iron-improved sand layer and above the adsorption layer, and the iron-improved sand layer and the adsorption layer are communicated through upright pipes which extend to the position above the covering layer; the treating units sequentially comprise the covering layer, the vegetation layer, the adsorption layer, the iron-improved sand layer and the submersion layer from top to bottom; hardwood bark, gravel in light color and sand are arranged on the cover layer; the vegetation layer comprises preferable plants and degrading bacterium immobilized particles; the adsorption layer comprises modified peat containing a proper amount of sludge and modified zeolite; the iron-improved sand layer can keep Fe3<+> and maintain an aerobic condition; the submersion layer has wood flour, newspaper and other organic matters and keeps an anaerobic condition. Various pollutants in the rainwater runoff are effectively removed due to special interlayer arrangement of the facility and combination of biological enhanced biological treatment and adsorption treatment.

Biologically strengthened processing of industrial sewage is carried out by adopting a microbial immobilization technology in order to improve the removal rate of organic materials, which are difficult to degrade, and thus the processing efficiency of industrial sewage is increased. Through double cross-linking of PVA-boric acid and sodium alginate-calcium chloride, high effective degrading bacteria, which have been screened and cultured, are fixed on carriers. (See attached drawing) The prepared immobilized microbes can be directly added into a biological processing structure, which is used for processing industrial sewage, for biological strengthening. The immobilized microbes, which are prepared by double cross-linking of PVA-boric acid and sodium alginate-calcium chloride, tightly bind the carriers, have the advantages of high mechanical strength, wear resistance, and can be repeatedly used for a long time. Immobilized high effective degrading bacteria in the biological processing structure can maintain very high biomass and biological activity, and are difficult to lose, so that the processing efficiency and load of the biological processing structure are improved, and the volume and occupied area of the biological processing device are reduced. Furthermore, the biological degrading ability of the added bacteria is also improved after the immobilization treatment.

The invention discloses a method for improving terramycin impact resistance of anammox sludge. The method comprises the following steps: inoculating anammox particle sludge in an anaerobic sludge reactor, performing operation treatment on ammonia nitrogen-containing wastewater, and monitoring the terramycin content in the wastewater on line, wherein terramycin impact exists when the terramycin content is increased to be 500mg/L or more; and adding fresh anammox particle sludge into the anaerobic sludge reactor, wherein the mass of volatile suspended solids in the fresh anammox particle sludge accounts for 3-8 percent of the mass of volatile suspended solids contained in the original particle sludge in the anaerobic sludge reactor. With the adoption of a biological enhancement mode, the restoration time of the anaerobic sludge reactor can be shortened, and the terramycin impact resistance stability of the reactor is enhanced.

The present invention relates to hydrogen preparation. Material is replenished continuously to spawn fermenting tank to ensure the continuous production of hydrogen producing spawn; nutritious liquid is pumped continuously from the material replenishing tank to the fermenting tank to ensure the continuous supply of spawn culture medium in the nutritious liquid replenishing rate of 0.5/day; and the spawn cultured continuously in the fermenting tank is pumped continuously with a metering pump fermenting biological hydrogen producing reactor in the rate fitting the nutritious liquid replenishing rate of the fermenting tank. The continuous culturing process has raised hydrogen producing capacity and improved fermenting bacteria structure, and is suitable for industrial hydrogen production.

A biological grid water body fortifying purification and its apparatus are disclosed. It forms self-fixing float filler, microbial membrane and spiral shell fed in ecological contact oxidation pool, biological filter area with lifting-flow suspended filler is set at outlet, the ecological contact oxidation pool is mad up of reinforced concrete structure, mesh blind flange on top of which is covered with vegetation layer on pool top. Its advantages include less mud output, better water quality purifying effect and low cost.

The invention belongs to fields of environmental protection science and engineering, and in particular relates to a sewage treatment method, namely a sewage treatment method combining biological reinforced flocculation, speed-changing biofilters and artificial wetlands. Sewage is pretreated and is treated by the biological reinforced flocculation, then is treated by the speed-changing biofilters,and finally is treated by the artificial wetlands; and yielding water accords to a standard and is discharged or recycled, partial sludge flows to a flocculation pond reversely, and the residual sludge is discharged from the bottom of a settling pond. The sewage treatment method is combination process technology for sewage treatment, which is suitable for the sewage treatment of small towns and has less land occupation, investment saving, low operating cost, high efficiency and convenient operation and management, and can be widely applied to the sewage treatment.

The invention discloses a fed culture method of hydrogen-producing bacteria and a biological enhancing method of a biological hydrogen producing system, which relates to a method for enhancing bacterial culture and the biological hydrogen producing system. The method solves the problems that the existing fermentation method has the defects of long startup time of a reactor, low hydrogen productivity and sludge loss during continuous operations of the biological hydrogen production. The method comprises the steps: A. hydrogen-producing bacteria are inoculated in nutrient solution in a fermentation tank for anaerobic fermentation; B. after the anaerobic fermentation, a part of fermentation solution is put into a biological hydrogen producing reacting device by a metering pump, and then fresh nutrient solution, which has the same volume as a part of fermentation solution put into the biological hydrogen producing reacting device, is added into the fermentation tank for ongoing anaerobic fermentation, thereby completing the fed culture of hydrogen-producing bacteria and the biological enhancement of the biological hydrogen producing system. The method shortens the startup time of the biological hydrogen producing reactor during the continuous operation to 15 days to 20 days, and increases the hydrogen productivity by 10 percent to 15 percent, thus avoiding the loss of active sludge.

The invention provides a rapid start-up process of an oily sewage biochemical treatment system in an oil field, belonging to the technical field of oily sewage treatment in the oil field. The process has the following beneficial effects: the earth surface clear water is firstly subjected to pre-start-up and then the oily sewage in the oil field is pumped to be subjected to start-up, the bacillus is selected as an oil removing bacterial agent and a biological strengthening agent formed by rapeseed cakes, wheat bran, inorganic phosphate and inorganic ammonium salt is used for strengthening the tolerance of the microorganisms to salinity and other biological inhibitors so as to effectively improve the activity of the microorganisms; in the start-up process of the biochemical system, the salinity increase speed is reasonably controlled, the microorganisms in the oily sewage with high salinity have high activity and large biomass while salinity increase is ensured and the adhesive capability of the microorganisms on the fillers is enhanced to achieve the aim of rapid start-up of the oily sewage biochemical treatment system in the oil field.

The invention relates to an integrated anaerobic ammonia oxidation device for treating urban sewage and an operation method thereof. The integrated anaerobic ammonia oxidation device comprises an urban sewage raw water tank, a high-load bioreactor, an intermediate regulating water tank, an integrated anaerobic ammonia oxidation reactor, a biological intensification reactor, a metering pool, an on-line monitoring detector and an automatic control system; the urban sewage raw water tank is connected with the high-load bioreactor through a high-load bioreactor water inlet pump; a drainage pipe of the high-load bioreactor is connected with the intermediate regulating water tank; the sewage in the intermediate regulating water tank is respectively connected with the integrated anaerobic ammonia oxidation reactor, the biological intensification reactor and the metering pool through a three-in-one water inlet pump. The integrated anaerobic ammonia oxidation device provided by the invention has the characteristics of being reasonable in structural design, high in degree of automation, reliable and stable in overall operation and the like, can effectively realize the nitrogen and phosphorus removal of the urban sewage and breaks through the problems of insufficient carbon source, low nitrogen removal rate, high residual sludge yield and the like when a traditional nitrogen and phosphorus removal process is adopted for treating the urban sewage.