Continuous flow culture method of industrial biological hydrogen preparing spawn and biological hydrogen preparing system reinforcing method

A technology of biological hydrogen production and system strengthening, applied in the direction of bacteria and fermentation, etc., can solve the problems of small-scale hydrogen production and inability to produce hydrogen in large quantities

Active Publication Date: 2006-05-17
HARBIN INST OF TECH
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  • Summary
  • Abstract
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Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that the existing hydrogen production method is still relatively primitive, the production scale of hydrogen production is small, and the hydrogen

Method used

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Experimental program
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specific Embodiment approach 1

[0004] Specific embodiment one: this embodiment mode is realized through the following steps: one, the cell concentration is 5 * 10 9 A / L of hydrogen-producing bacteria liquid, inoculated into the strain nutrient solution at a volume ratio of 1%, placed in a constant temperature air bath oscillator, the rotation speed of the oscillator was 120 rpm, and the temperature was controlled at 34-36 ℃, cultivated for 20-30 hours, and obtained the strain sample of the first-level expanded culture; 2. Inoculated the strain cell suspension of the first-level expanded culture into the fermenter containing the sterilized liquid nutrient solution with a volume ratio of 1-3% Carry out secondary expansion culture in the fermentation tank, the temperature inside the fermenter is controlled at 34-36 °C, the stirring speed is 120 rpm, and the hydrogen-producing bacteria are cultivated in the fermenter for 40-50 hours before continuous cultivation and continuous feeding; three. , at two effective...

specific Embodiment approach 2

[0005] Specific embodiment 2: In step 1 of this embodiment, the temperature is controlled at 35°C. Other methods and steps are the same as those in the first embodiment.

specific Embodiment approach 3

[0006] Specific embodiment three: In step one of this embodiment, the incubation time is 24 hours. Other methods and steps are the same as those in the first embodiment.

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PUM

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Abstract

The present invention relates to hydrogen preparation. Material is replenished continuously to spawn fermenting tank to ensure the continuous production of hydrogen producing spawn; nutritious liquid is pumped continuously from the material replenishing tank to the fermenting tank to ensure the continuous supply of spawn culture medium in the nutritious liquid replenishing rate of 0.5/day; and the spawn cultured continuously in the fermenting tank is pumped continuously with a metering pump fermenting biological hydrogen producing reactor in the rate fitting the nutritious liquid replenishing rate of the fermenting tank. The continuous culturing process has raised hydrogen producing capacity and improved fermenting bacteria structure, and is suitable for industrial hydrogen production.

Description

technical field [0001] The invention relates to a method for preparing hydrogen. Background technique [0002] The non-renewable fossil energy such as petroleum, coal and natural gas has been exhausted due to the massive exploitation of human beings, and the sustainable development of human beings urgently needs the development of renewable follow-up energy. With the deepening of people's understanding of social sustainable development and environmental protection, the development of clean and renewable energy has become an urgent need for human beings. Hydrogen is favored in the energy industry due to its outstanding advantages such as cleanness, high efficiency, renewable, abundant resources, and easy storage and transportation. It is considered to be an important pillar of the world's energy system after the 21st century. In the future world energy system, hydrogen energy will play a pivotal role. In recent years, the development and utilization of hydrogen have received...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P3/00
Inventor 任南琪李建政秦智丁杰
Owner HARBIN INST OF TECH
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