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Method for treating soil or oil sludge severely contaminated by petroleum based on combination of sodium persulfate compound oxidation and microbial flora biological enhancement

A microbial flora, sodium persulfate technology, applied in microorganism-based methods, restoration of polluted soil, biochemical equipment and methods, etc., can solve problems such as unfavorable growth of plants and microorganisms, shorten the repair cycle, and achieve stable growth. , the effect of improving the degradation efficiency

Active Publication Date: 2016-12-07
SHAANXI NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, chemical treatment technology also has its limitations. For example, in the process of treatment and degradation, the treatment agent will treat other organic matter in the soil and change the adsorption structure and bioavailability in the soil, which is not conducive to the growth of plants and microorganisms. , cannot be effectively combined with bioremediation technology

Method used

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  • Method for treating soil or oil sludge severely contaminated by petroleum based on combination of sodium persulfate compound oxidation and microbial flora biological enhancement
  • Method for treating soil or oil sludge severely contaminated by petroleum based on combination of sodium persulfate compound oxidation and microbial flora biological enhancement
  • Method for treating soil or oil sludge severely contaminated by petroleum based on combination of sodium persulfate compound oxidation and microbial flora biological enhancement

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Experimental program
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Effect test

Embodiment 1

[0028] Bacillus sp., preserved in China Center for Type Culture Collection, strain preservation number CCTCC NO: AB2014248) and Acremonium sp. Y0997, preserved in China Center for Type Culture Collection, strain preservation number CCTCC NO: M 2016117, the storage date is March 14, 2016,) the solid microbial agent is a combination of bacterial flora, using sodium persulfate compound as a chemical oxidant, combined with the treatment of heavily oil-contaminated soil, consisting of the following steps:

[0029] (1) Collect heavily petroleum-contaminated soil with an initial total petroleum hydrocarbon content of 162,240 mg / kg near the oil well, place it in a treatment pool, crush it, and mix it evenly with a particle size of about 30-40 mm;

[0030] (2) Insert the oxidant sodium persulfate compound into the soil treated by step (1) in a ratio of 10g / kg, add water until the moisture content of the soil reaches 25%, mix well, and oxidize for 15 days, the sodium persulfate The comp...

Embodiment 2

[0037] Now use the solid bacterial agent of Phanerochaete sp.F0996, preserved in the China Center for Type Culture Collection, strain preservation number CCTCC NO:M 2016116, and the preservation date is March 14, 2016) as the flora combination , using sodium persulfate compound as a chemical oxidant, combined with the treatment of heavily oil-contaminated soil, consists of the following steps:

[0038] (1) Collect heavily petroleum-contaminated soil with a total petroleum hydrocarbon initial content of 170,000 mg / kg near the oil well and place it in a treatment pool, crush it to a particle size of about 30-40 mm, and mix well;

[0039] (2) Insert the oxidizing agent sodium persulfate compound into the soil treated by step (1) in a ratio of 8g / kg, add water until the moisture content of the soil reaches 15%, mix well, and perform oxidation treatment for 20 days. The complex is made by mixing sodium persulfate, calcium peroxide and ferrous sulfate in a molar ratio of 5:0.5:2.5; ...

Embodiment 3

[0047] Using sodium persulfate compound as a chemical oxidant, combined with the mixed flora of Bacillus and Acremonium to carry out intensive joint treatment of heavily oil-contaminated oil sludge, which consists of the following steps:

[0048] (1) Collect heavily oil-contaminated oil sludge with a total petroleum hydrocarbon content of 100,000 mg / kg near the oil well and place it in the treatment tank, crush it to a particle size of about 30-40 mm, and mix well;

[0049] (2) the oxidant sodium persulfate compound is inserted in the oil sludge processed through step (1) in the ratio of 12g / kg, the sodium persulfate compound is made of sodium persulfate, calcium peroxide and ferrous sulfate with 5:0.5: Mixed at a molar ratio of 2.5, then added heavy oil cleaning agent KD-L315 as a surfactant according to the addition amount of 1200mg / kg (sludge), and added water until the moisture content of the sludge reached 30%, mixed well, and oxidized for 10 days;

[0050] (3) Insert the...

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Abstract

The invention discloses a method for treating soil or oil sludge severely contaminated by petroleum based on combination of sodium persulfate compound oxidation and microbial flora biological enhancement. Contaminated soil or oil sludge is oxidized first with a sodium persulfate compound as the oxidizing agent in the presence of a surfactant, and then a mixed flora of bacillus sp. and acremonium sp. Y0997 or phanerochaete sp. F0996 is added twice continuously, so that intensified combined treatment of soil or oil sludge is achieved through intensified combination of chemical oxidization and fungus-bacterium flora biological sequences. In this way, the degrading efficiency of soil or oil sludge severely contaminated by petroleum can be improved remarkably, and repair period can be shortened greatly.

Description

technical field [0001] The invention belongs to the technical field of chemical and biological remediation of heavily oil-contaminated soil or oil sludge, and specifically relates to a method for combined treatment of heavily oil-polluted soil or oil sludge by chemical oxidation and fungal-bacterial flora bioaugmentation in oilfield mining areas. technical background [0002] At present, with the development of domestic and foreign petroleum industries, the scope of petroleum-contaminated soil is constantly expanding. It is an urgent problem to be solved in the process of oil-contaminated soil treatment to treat oil-contaminated soil so that it can achieve the effect of recovery and utilization in a short period of time. For the treatment of pollutants, which method is the most suitable, in addition to considering the location of the pollutants, the amount of pollutants, the quality of the treatment effect, the length of time required and the length of the treatment, and oth...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14B09C1/08B09C1/10C02F3/34C12R1/07C12R1/645C02F103/10
Inventor 马小魁毛东霞周波李郁郭丹丹李亭亭马瑶宋双红
Owner SHAANXI NORMAL UNIV
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