Bio-augmentation treatment sludge in-situ reduction method
A bioaugmentation and sludge technology, applied in the field of environmental biology, can solve problems such as unsatisfactory effects, difficult growth control, and high requirements for process management and control, and achieve the effects of high promotion and application value, low operating costs, and low investment
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Embodiment 1
[0030] Using beef extract peptone medium, the formula is beef extract 3g, peptone 10g, Nacl 5g, water 1000mL, pH7.4~7.6, and the yeast, Bacillus, Pseudomonas, photosynthetic bacteria and micrococcus 5 strains obtained by screening were in Cultivate separately in a 10L fermenter for 48 hours, and each obtain 6L of bacterial liquid; inoculate 30L of the above-cultured bacterial mixture into a 100L solid fermenter with an inoculum size of 30% and a solid medium composed of bran 50%, corn Core powder 10%, soybean meal 15%, starch 2%, urea 2%, sodium glutamate 0.5%, ammonium sulfate 2%, KH 2 PO 4 0.8%, add water to adjust the water content to 45% by weight, adjust the pH to 7.2, and ferment for 48 hours at a temperature of 36°C with ventilation and stirring, and then vacuum-dry to make a solid bacterial agent; the number of living bacteria detected by agar-coated plate culture is 10 14 CFU / g.
Embodiment 2
[0032] Adopt LB liquid culture medium, formula is peptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, culture 5 types of bacterial strains separately in 10L fermenter, culture time 24h, each obtains 4L of bacterial liquid; Inoculate 20L of the above-cultured bacterial mixture into a 100L solid fermenter with an inoculum size of 20%. The solid medium consists of 30% bran, 20% corncob flour, 5% soybean meal, 0.5% starch, 0.1% urea, corn Sodium Amino Acid 2%, Ammonium Sulfate 0.2%, KH 2 PO 4 0.1%, add water to adjust the water content to 65% by weight, adjust the pH to 6.0, and ferment for 24 hours at a temperature of 28°C with ventilation and stirring, and then vacuum-dry to make a solid bacterial agent; the number of living bacteria is 10 by ordinary agar plate culture 9 CFU / g.
Embodiment 3
[0034] The method is used for the in-situ reduction of sludge in the secondary treatment of urban domestic sewage activated sludge process, using anoxic / anaerobic / aerobic (AO / A 2 O) technology, influent water quality average COD 340 mg / L, BOD 155 mg / L, NH3-N 30 mg / L, TN 41 mg / L, TP 4.5 mg / L, SS 107 mg / L, water volume 25,000 t / d, the total hydraulic retention time is 14 h. The average effluent quality of the original process is COD 32 mg / L, BOD 7.6 mg / L, NH3-N 5 mg / L, TN 13 mg / L, TP 0.4 mg / L, SS 8 mg / L, and the total amount of residual sludge generated is 5.5 t / d (after plate and frame filter press, 85% water content). Do not change the original process for biointensification treatment. First, add 5 times the functional bacteria agent to the sterile water for aeration and activation for 2 days, and then use a peristaltic pump to feed it to the anoxic water inlet and the aerobic water inlet respectively, and the flow rate is 10 ppm. After continuous feeding for 2 w, the prope...
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