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This invention belongs to the field of microbial application technology, specifically relating to a method for preparing fermented *Lactobacillus limnoxicamis* extracellular vesicles, the product, and its applications, particularly the application of these fermented *Lactobacillus limnoxicamis* extracellular vesicles and their lyophilized formulations in the preparation of products with antioxidant or anti-aging effects. In this invention, *Lactobacillus limnoxicamis* is inoculated into a modified extracellular vesicle induction medium for fermentation. After culture, a two-stage solid-liquid separation is performed, and the supernatant is collected to obtain the extracellular vesicle processing solution. This solution is purified using a capillary electrophoresis system, and the positive extreme liquid is collected and filtered to obtain the purified product of fermented *Lactobacillus limnoxicamis* extracellular vesicles. The extracellular vesicles derived from fermented *Lactobacillus limnoxicamis* prepared by this invention can effectively scavenge free radicals. Somatic cell experiments have demonstrated its excellent anti-aging properties, and the transformation and application research of *Lactobacillus* extracellular vesicles has explored its development potential in the field of anti-aging products.