Preparation method of anti-fatigue cordyceps militaris tomato ferment

A technology for tomato enzyme and cordyceps militaris, which is applied in the field of preparation of cordyceps militaris tomato enzyme, can solve the problems of low enzyme content and inability to meet people's normal needs and the like

Inactive Publication Date: 2016-05-04
沈阳园康天然生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the enzyme content in daily fo

Method used

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  • Preparation method of anti-fatigue cordyceps militaris tomato ferment

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[0020] Example 1:

[0021] Step 1: Prepare yeast seed liquid:

[0022] Seed tank expansion medium: YPD liquid medium, the mass percentage of which is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and the rest is water. Put YPD liquid medium into 0.5M 3 In the seed tank, sterilize at a high temperature of 115°C for 30 minutes; under aseptic operation, dissolve 1.5 kg of dry yeast powder in 3 times sterile water to form a bacterial liquid, and put the bacterial liquid into the serum bottle and Inoculated to 0.5M 3 In the seed tank, pass sterile compressed air at 28°C with a ventilation rate of 0.2 cubic meters per minute, and cultivate for 20 hours. When the bacteria grow to the end of the logarithmic phase, stop the culture. Repeat the above steps to obtain the desired quality of yeast Seed liquid of bacterial liquid;

[0023] Step 2: Prepare lactic acid bacteria seed liquid:

[0024] Seed tank expansion medium: malt compound juice enrichment medium, its mass percentage is 10% wo...

Example Embodiment

[0031] Example 2:

[0032] Step 1: Prepare yeast seed liquid:

[0033] Seed tank expansion medium: YPD liquid medium, the mass percentage of which is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and the rest is water. Put YPD liquid medium into 0.5M 3 In the seed tank, sterilize at a high temperature of 115°C for 50 minutes; under aseptic operation, dissolve 1.5 kg of dry yeast powder in 3 times aseptic water to form a bacterial liquid, and put the bacterial liquid into the serum bottle. Inoculated to 0.5M 3 In the seed tank, pass sterile compressed air at 30°C with an aeration rate of 0.3 cubic meters per minute. Cultivate for 30 hours. When the bacteria grow to the end of the logarithmic phase, stop the culture. Repeat the above steps to obtain the desired quality of yeast Seed liquid of bacterial liquid;

[0034] Step 2: Prepare lactic acid bacteria seed liquid:

[0035] Seed tank expansion medium: malt compound juice enrichment medium, its mass percentage is 10% wort, 0.5% ...

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Abstract

The invention provides a preparation method of anti-fatigue cordyceps militaris tomato ferment. The preparation method comprises the following steps: step one, preparing a yeast strain liquid; step two, preparing a lactobacillus strain liquid; step three, culturing fermentation broth; step four, subjecting the fermentation broth to a centrifugation treatment to obtain an original liquid of ferment; step five, mixing a cordyceps militaris extract and the original liquid of ferment obtained in the step four according to a mass ratio of 20:1000, making the mixture stay in an environment with a temperature of 115 DEG C for 30 to 50 minutes so as to kill the bacteria, and finally filling the ferment into different containers.

Description

Technical field [0001] The invention belongs to the technical field of biological preparations, and particularly relates to a preparation method of anti-fatigue Cordyceps militaris tomato enzyme. Background technique [0002] Enzymes, also known as "enzymes", are polymer materials with biocatalytic functions. Almost all cell activities require the participation of enzymes. In modern society, with the acceleration of people's life rhythm and the intensification of environmental pollution, the content of enzymes in people's bodies is decreasing, which in turn reduces the body's immunity and metabolic rate, making people more likely to get sick. At present, the enzyme content in daily food is too low to meet people's normal needs. In order to meet the diversity, balance and easy absorption of modern people's nutritional needs, the production of efficient enzyme products is imminent. Summary of the invention [0003] In order to overcome the defects in the prior art, the purpose of...

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Application Information

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IPC IPC(8): A23L33/00A23L31/00C12N1/20C12N1/16C12R1/10C12R1/46
CPCC12N1/16C12N1/20A23V2002/00A23V2400/51A23V2400/249A23V2250/21A23V2200/30
Inventor 不公告发明人
Owner 沈阳园康天然生物科技有限公司
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