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Uses of Arctium lappa oligofructose in medicament or health care food

A technology of burdock oligofructose and health food, which is applied in application, food preparation, drug combination and other directions

Inactive Publication Date: 2008-11-05
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing research results have shown that it can promote the growth of bifidobacteria and the proliferation of mouse splenocytes in vitro, but there is no report on its effects on improving the body's immunity, anti-tumor and anti-fatigue after oral administration

Method used

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  • Uses of Arctium lappa oligofructose in medicament or health care food
  • Uses of Arctium lappa oligofructose in medicament or health care food
  • Uses of Arctium lappa oligofructose in medicament or health care food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: Effect of BFO administration in vitro on mouse macrophage acid phosphatase activity

[0012] Mice were intraperitoneally injected with 2ml of 3% liquid thioglycollate medium, and killed by cervical dislocation 3 days later, aseptically took mouse peritoneal macrophages to prepare cell suspension; the macrophage suspension was added to a 96-well cell culture plate, 100 μl per well. In the experiment, a control group, a drug (final concentration of 6.25, 12.5, 25, 50, 100, 500, and 1000 μg / ml) stimulation group, and an LPS (final concentration of 1 μg / ml) stimulation group were set up, and three replicate wells were set up for each concentration in each group. . Place CO at 37°C, 5% CO2 2 Incubate for 24 hours in the incubator. Take it out, discard the supernatant, add 100 μl of pNPP solution to each well, mix well, place it at 37°C for 30 min, take it out, and immediately add 10 μl of 1.0 mol / L NaOH solution to terminate the reaction. Read the OD value of ...

Embodiment 2

[0017] Example 2: Immunomodulatory effect of burdock fructooligosaccharide administered in vivo on normal mice

[0018] 100, 250, 500 and 1000mg / kg.day BFO was administered orally to normal mice for 7 consecutive days, and the effects on NO production, acid phosphatase activity and phagocytic activity of normal mouse macrophages were detected.

[0019] Determination of NO production by macrophages: On the fifth day of the experiment, 2 ml of 3% thioglycollate medium was injected intraperitoneally to sensitized mice. On the 8th day, the mice were killed by bloodletting, and the peritoneal macrophages were aseptically collected to prepare a cell suspension of 1×10 6 cells / ml, add 100 μl of cell suspension and LPS (final concentration: 1 μg / ml) to each well of a 96-well plate, and set up triplicate wells. At 37°C, 5% CO 2 CO 2 Culture in the incubator for 24 hours, take it out, carefully pipette 50 μl of the supernatant from each well and place it in another 96-well cell cultu...

Embodiment 3

[0033] Example 3: In vivo administration of burdock fructooligosaccharides to the immune regulation of immunocompromised mice

[0034] In this experiment, cyclophosphamide was used to create an immunocompromised mouse model, and the mice were intraperitoneally injected with cyclophosphamide 75 mg / kg.d on the first, second, and third days of the experiment to form an immunocompromised model. 100, 250, 500 and 1000mg / kg.day BFO was administered orally to immunocompromised mice for 7 consecutive days, and the effects on NO production, acid phosphatase activity and phagocytic activity of normal mouse macrophages, splenocytes Proliferation, serum hemolysin production, and B cell-mediated hemolysis.

[0035] Determination of macrophage acid phosphatase activity: On the fifth day of the experiment, 2 ml of 3% thioglycolate medium was injected intraperitoneally to sensitized mice. On the 8th day, the mice were killed by bloodletting, and the peritoneal macrophages were aseptically co...

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Abstract

The invention provides an application of burdock fructooligosaccharide in the preparation of drugs for improving immunity, anti-tumor or anti-fatigue or health care foods such aspects. The burdock fructooligosaccharide can improve activity of macrophages, improve NO production of the macrophages and enhance phagocytosis of the macrophages and the activity of acid phosphatase; the burdock fructooligosaccharide can improve the content of hepatic glycogen and muscle glycogen, reduce the metabolic wastes of blood lactate, blood urea nitrogen and so on, enhance the exercise endurance capacity and have effective anti-fatigue property; and the burdock fructooligosaccharide has the significant effects of preventing and treating tumors.

Description

technical field [0001] The present invention relates to the application of burdock extract - burdock fructooligosaccharide (hereinafter referred to as BFO) in medicine or health food, especially relates to the preparation of burdock fructooligosaccharide for improving immunity, anti-tumor or anti-fatigue medicine or health food Applications. Background technique [0002] Burdock fructooligosaccharide is a low molecular weight inulin derived from the root of the plant burdock. Inulin from Compositae plants has been confirmed as a functional food component in human and animal experiments, which can affect the physiological and biochemical processes of the body, promote health and reduce the risk of various diseases. Functional studies have shown that inulin is a prebiotic substance that can stimulate the body's immune system, reduce the level of harmful bacteria in the intestines, reduce the risk of osteoporosis by increasing the absorption of minerals, especially calcium, an...

Claims

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Application Information

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IPC IPC(8): A61K31/702A61P35/00A61P37/04A61P25/26A23L1/30A23L33/105
Inventor 陈靠山刘少芳张鹏英姚佳余丽珍
Owner SHANDONG UNIV
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