7 results about "Pentacyclic triterpenoids" patented technology

This invention discloses a class of pentacyclic triterpenoid derivatives with diverse B-ring modifications, their preparation methods, and applications. This invention utilizes a chemical enzymatic method to expand the chemical space of the B-ring in pentacyclic triterpenoids, obtaining a series of pentacyclic triterpenoid derivatives with diverse B-ring modifications. Pharmacological activity screening of these pentacyclic triterpenoid derivatives revealed their good tumor-suppressive activity, providing a promising application prospect for the prevention and treatment of tumors. This invention achieves the efficient synthesis of pentacyclic triterpenoid derivatives with diverse B-ring modifications, structures that are difficult to obtain using traditional chemical methods. Systematic antiproliferative activity screening identified candidate molecules with broad-spectrum and highly efficient inhibitory effects against various human tumor cell lines. These molecules show good drug development potential, providing novel lead compounds for the innovative development of anticancer drugs.

The present application belongs to the technical field of quality control of traditional Chinese medicine, and particularly relates to a quality control method of Ledum palustre L. The quality control method provided by the present application comprises the following processes: S1, identification: thin layer chromatography identification is respectively performed by taking ursolic acid, hyperoside, rutin, aesculetin and aesculin as the control; S2, inspection: the moisture content, total ash content, acid insoluble ash content and alcohol-soluble extract content of the medicinal material are determined; S3, content determination: the total content of volatile oil, three five-ring triterpenes (betulinic acid, oleanolic acid and ursolic acid) and two flavonoid glycosides (hyperoside and preussin) in the medicinal material is determined by using high performance liquid chromatography. The present application combines multi-index component analysis and thin layer chromatography identification, has strong specificity and good reproducibility, can comprehensively and effectively control the quality of Ledum palustre L., and ensures the medicinal effectiveness and safety of Ledum palustre L.

The application discloses functions and uses of a kind of pentacyclic triterpenoid compounds in preventing and / or treating neurodegenerative diseases, and the compounds are obtained by isolating and extracting from Cynomorium songaricum Rupr medicinal materials.The inventors found that the compounds have significant protective effects on glutamic acid-induced nerve cell injury and oxygen-glucose deprivation-induced nerve cell injury during pharmacological activity evaluation of the compounds, and thus can be used for treating and preventing neurodegenerative diseases such as stroke, brain injury, amyotrophic lateral sclerosis, Huntington's disease, Parkinson's disease and Alzheimer's disease, and can be developed into drugs.

The application discloses application of polyphosphate-dependent glucokinase in glycosylation modification of pentacyclic triterpenoid compounds, a sugar group donor of the glycosylation modification is sucrose, the polyphosphate-dependent glucokinase is used for converting byproduct fructose into fructose-6-phosphate, and the polyphosphate-dependent glucokinase is CgPPGK with an amino acid sequence as shown in SEQ ID NO:1 or Asp-PPGK with an amino acid sequence as shown in SEQ ID NO:2. The polyphosphate-dependent glucokinase CgPPGK or Asp-PPGK is coupled to a traditional UDP-glucose regeneration system in the application, a coupled three-enzyme UDP-glucose regeneration system is formed, consumption of fructose is realized, more UDP-glucose is generated in the reaction, glycosylation efficiency and sucrose utilization rate are improved, and the glycosylation modification capacity for a substrate is obviously higher than that of an original traditional UDP-glucose regeneration system.

The application discloses functions and uses of a kind of pentacyclic triterpenoid compounds in preventing and / or treating neurodegenerative diseases, and the compounds are obtained by isolating and extracting from Cynomorium songaricum Rupr medicinal materials.The inventors found that the compounds have significant protective effects on glutamic acid-induced nerve cell injury and oxygen-glucose deprivation-induced nerve cell injury during pharmacological activity evaluation of the compounds, and thus can be used for treating and preventing neurodegenerative diseases such as stroke, brain injury, amyotrophic lateral sclerosis, Huntington's disease, Parkinson's disease and Alzheimer's disease, and can be developed into drugs.

The present invention provides a novel and improved method for preparing stable amorphous pentacyclic triterpenoids, compositions prepared using such pentacyclic triterpenoids, and a method for mitigating the effects caused by pro-inflammatory cytokines in mammals (including humans) by providing effective doses of pentacyclic triterpenoids treated according to the present invention. Furthermore, the present invention provides effective low-dose therapeutic compositions comprising pentacyclic triterpenoids treated according to the present invention.

The invention discloses a medicinal and edible complementary food for refreshing mind and restoring consciousness and a preparation method thereof, and belongs to the technical field of medicinal and edible complementary food. Comprising the following components in parts by weight: 10-15 parts of hawthorn, 10-25 parts of himalayan teasel root, 10-20 parts of akebia trifoliate, 5-15 parts of olean, 1-8 parts of sargassum, 1-8 parts of ulva pertusa, 3-10 parts of rhizoma polygonati, 1-5 parts of poria cocos, 1-5 parts of lentils, 1-4 parts of chickpeas, 2-8 parts of oat, 2-10 parts of chestnut kernels, 1-6 parts of pumpkin seeds, 1-5 parts of grapefruit seeds, 2-8 parts of honey, 5-10 parts of cow milk and 1-2 parts of vitamins. 1-2 parts of mineral substances and 1-5 parts of beta-maltodextrin. Through scientific proportioning and reasonable compatibility of traditional Chinese medicines, the synergistic effect of various active substances is realized, through reasonable compatibility, the prepared medicinal and edible complementary food can play a more remarkable anti-fatigue role, loss of pentacyclic triterpenoids is effectively prevented through double-layer embedding, and the content of active ingredients is ensured. Animal experiments prove that the medicinal and edible complementary food can improve exercise tolerance and fatigue resistance of mice.