Method for preparing low molecular weight seaweed polysaccharide sulfate

A seaweed sulfated polysaccharide and low molecular weight technology, applied in the field of marine biology, can solve the problems of acid polysaccharide sulfate radical shedding, high requirements on equipment and equipment, and cannot be applied to industrial production, and achieves improved solubility, less reagent dosage, and uniformity. Good results

Inactive Publication Date: 2005-05-18
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

[0003] The current problem is that most of the natural seaweed sulfated polysaccharides are polysaccharides with large molecular weight
[0006] 2. Alkaline hydrolysis, under alkaline conditions, often causes the modification of acidic polysaccharides and the shedding of sulfate groups, wh

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  • Method for preparing low molecular weight seaweed polysaccharide sulfate
  • Method for preparing low molecular weight seaweed polysaccharide sulfate
  • Method for preparing low molecular weight seaweed polysaccharide sulfate

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example 1

[0044] Example 1. After degrading the Porphyra polysaccharide, it was centrifuged at 6000r / m for 20min, and the supernatant was dialyzed for 24h to remove residual ascorbic acid and hydrogen peroxide; then concentrated and dried to obtain the product. The molecular weight determination method of product: be to use gel permeation chromatography, differential detector, adopt 0.7%Na 2 SO 4 As the mobile phase, the column temperature is 35 degrees Celsius, and the above data are measured.

[0045] Example 2——5 Seaweed Sulphated Polysaccharide Degradation Example, its polysaccharide preparation method is the same as that of Example 1, and the concentration of polysaccharide, the amount of ascorbic acid and hydrogen peroxide, the reaction time and temperature are shown in Table 1. After degradation, centrifuge at 6000r / m for 20min, and dialyze the supernatant for 24h to remove residual ascorbic acid and hydrogen peroxide; then concentrate and dry to obtain the product.

[0046] Ex...

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Abstract

The present invention relates to the preparation process of low molecular weight seaweed polysaccharide sulfate. The preparation process includes adding ascorbic acid and hydrogen peroxide into the solution of natural seaweed polysaccharide sulfate as material, with the added amount being 0.1-50 mmol/L and 0.1-100 mmol/L and the ratio being 1 to 0.1-10, constant degradation under controlled temperature for 0.5-3 hr, dialysis or ultrafiltering, decompression concentration and freeze drying to obtain low molecular weight seaweed polysaccharide sulfate product of molecular weight 4-100 KDa. The present invention has the advantages of fast degradation speed, low reagent consumption, simple process, concentrated molecular weight distribution and other advantages. The low molecular weight seaweed polysaccharide sulfate product has controllable molecular weight, greatly raised dissolubility and bioactivity.

Description

technical field [0001] The invention relates to the research and development of marine medicines, in particular to a preparation method of low-molecular-weight seaweed sulfated polysaccharides, which belongs to the technical field of marine biology. Background technique [0002] Sulfated polysaccharides are polysaccharides in which one or more hydroxyl positions in the polysaccharide constituent units are sulfated. In the plant kingdom, only algae have natural sulfated polysaccharides. Seaweed sulfated polysaccharide is a kind of polysaccharide unique to seaweed. Seaweed sulfated polysaccharides have a wide range of biological activities, including antiviral, antitumor, growth factor effects, immune system effects, and anticoagulant activity. It has been reported that fucoidan sulfate and carrageenan have anti-HIV-1 cytotoxic activity (CD4 + T lymphocytes), can inhibit HIV-1 on CD4 + Infection of the lymphoid cell line MT-4. In addition, seaweed sulfated polysaccharides...

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Application Information

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IPC IPC(8): A61K31/734C08B37/04
Inventor 张全斌赵婷婷牛锡珍张虹綦慧敏徐祖洪李智恩
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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