32results about How to "Good separation and enrichment effect" patented technology

The invention discloses a method for separating and culturing mouse primitive spermatogonia. In the method, the mouse primitive spermatogonia are separated by utilizing the characteristics of neutral protease, such as mild digestibility performance, high efficiency and small stimulation to cells; the step of enrichment and subculture of the mouse primitive spermatogonia are organically combined; and adherent mouse primitive spermatogonium colony is digested by utilizing the neutral protease, so a better enrichment effect is achieved. The method for separating and culturing the mouse primitive spermatogonia is characterized by simple separating and culturing operation, short period, good effect and low cost.

The invention provides a complex tracer agent for oil field high-temperature hypersalinity block interwell monitoring and the application thereof. The tracer agent comprises one or more of chlorinated hydroxylamine erbium, hydroxylamine gadolinium chloride, chlorinated hydroxylamine dysprosium, chlorinated hydroxylamine ytterbium and chlorinated hydroxylamine holmium, and the tracer agent is formed by a tracer element and a complexing agent in a complexing mode. The tracer element is selected from one or more of erbium, gadolinium, dysprosium, ytterbium and holmium, the complexing agent is hydroxylammonium chloride, and the molar ratio between the tracer element and the complexing agent is 1:8-1:9 and preferably is 1:8. The tracer agent can resist high temperature (250 DEGC-300 DEG C) and hypersalinity (5000mg / L-20000mg / L). The invention further provides a tracer method for conducting the oil field high-temperature hypersalinity block interwell monitoring by means of the tracer agent, and the method comprises the process of conducting separation and enrichment on the tracer agent in a taken sample.

The invention relates to a method for efficiently separating and purifying multiresidue sulfonamide antibiotics in a biological sample. The method is characterized by comprising the following steps of: (1) preparing a molecular imprinted polymer, comprising the steps of burdening according to the mol ratio of template molecule to functional monomer to cross linking agent of being 1: (2-8): 25 and obtaining the molecular imprinted polymer with special identification function to the sulfonamide antibiotics by adopting a body polymerization method; (2) weighing certain amount of biological sample to be separated into a mortar, grinding the biological sample by the mortar, adding right amount of the molecular imprinted polymer, and further homogenating to obtain a homogenated sample, wherein the weight ratio of the biological sample to be separated to the molecular imprinted polymer is 2: (1-5), and (3) placing the homogenated sample in a solid phase extraction column, sequentially rinsing with 2.0-4.0ml of water, 2.0-4.0ml of 10wt% acetonitrile aqueous solution, and then eluting with 3.0-5.0ml of methanol / methanoic acid mixed solution; and separating the sulfonamide antibiotics from the biological sample to be separated by being instantly dissolved in the methanol / methanoic acid mixed solution. The method provided by the invention has wide selectivity and good separation and purification effect.

The invention discloses a separation and enrichment method for lithium isotopes. The method comprises the steps that S1, an extracted organic phase is prepared, wherein a crown ether derivative represented as the formula A or the formula B is taken as an extraction agent, and the extraction agent, hydrophobic ionic liquid and a diluent are mixed to obtain the extracted organic phase; S2, an extracted water phase is prepared, wherein water-soluble lithium salt is prepared into a lithium salt solution, and the lithium salt solution is taken as the extracted water phase; S3, after the extracted organic phase and the extracted water phase are fully mixed and extracted, phase splitting is conducted, and a load organic phase and extraction raffinate are obtained; S4, the load organic phase is burned for 30-200 min at the temperature of 400-1,000 DEG C, and a slag phase is obtained; S5, the slag phase is dissolved with water, solid-liquid separation is conducted, and 6Li enrichment liquid isobtained; S6, the 6Li enrichment liquid is taken as the extracted water phase, and the steps of S3-S5 are repeated. The invention provides a brand new technology method without adopting the reverse extraction operation based on a crown ether extraction system. The technology method is a quick cross flow superposing multi-level cascade separation method capable of enriching the methods of conducting separation and enrichment treatment on lithium isotopes.

The invention relates to a method for separating germanium from a levextrel resin by adsorptive extraction. The technical scheme is as follows: the method comprises the following steps: uniformly mixing an AB-8 macroporous resin, N235 and an impregnant, stirring in a 50-70 DEG C water bath for 1-3 hours, and heating to 80 DEG C to dry off the impregnant, thereby obtaining an N235 levextrel resin; putting a germanium-containing solution, the N235 levextrel resin and tartaric acid in a container, regulating the pH value to about 2.0, oscillating and stirring for 80-100 minutes, and filtering to obtain a germanium-adsorbed N235 levextrel resin; and adding an eluting agent into the germanium-adsorbed N235 levextrel resin, oscillating and stirring for 80-100 minutes, filtering, and taking the filtrate. The method is simple, and has favorable separation and enrichment effects; the extraction rate can reach 89% above, and the eluting rate can reach 90% above; and it is proved that the N235 levextrel resin only adsorbs germanium, so the purity is high, and the germanium can be effectively separated from impurity ions.

The invention discloses a method for suction extraction for separating germanium based on a dynamic adsorption method. The method comprises the following steps: filling a glass tube with an epoxy acrylic acid extraction and leaching agent so as to prepare an epoxy acrylic acid extraction and leaching resin column; putting a germanium-containing solution and tartaric acid into a container, uniformly stirring, adjusting the pH value to 1.0-1.5 so as to obtain a mixed solution, dropping the mixed solution into the epoxy acrylic acid extraction and leaching resin column by using an acid buret at aflowing speed of 15-20 droplets / minute, collecting an effluent, and circulating and adsorbing the effluent for 6-8 times. According to the method, an extraction agent is chelated inside an epoxy acrylic acid resin by using a suspension polymerization method, the formed extraction and leaching resin has the advantages of being small in extraction agent loss and recyclable, and in addition, the extraction and leaching resin still has a very high extraction rate after 8-10 times of repeated use. The method disclosed by the invention is simple, low in manufacturing cost, clean and environmental-friendly and good in separation and enrichment effect, and the extraction rate of the method disclosed by the invention can be up to 98% or greater.

The invention relates to a sulfonation method of natural zeolite for adsorption and recycling of rare earth ions. The method comprises the following steps of firstly, acidizing the natural zeolite, then conducting sulfhydrylation grafting on the surface of the acidized natural zeolite, oxidizing sulfydryl on the surface of the natural zeolite subjected to sulfhydrylation, and finally, acidizing the obtained product in an acidic solution so as to obtain the sulfonated natural zeolite. The sulfonated zeolite not only has the original high specific surface area of the zeolite but also has functional groups with chelation action, and can gather and separate the rare earth ion furthest under the synergistic effect of two different adsorption modes.

The invention discloses a method used for high efficient separation and enrichment of tin from nickel-tin-iron alloy waste material. The method comprises following steps: the nickel-tin-iron alloy waste material is delivered into a metallurgical furnace, and is fused when the metallurgical furnace is heated; after full fusing, a vulcanizing agent is added into the furnace hearth of the metallurgical furnace via a spray gun, and sulfuration detinning reaction is performed; and high temperature smoke generated in the step of sulfuration detinning reaction is cooled, and smoke dust containing tin in the high temperature smoke is recycled. Process of the method is short, energy consumption is low, operation is simple, separation and enrichment effect of tin is obvious, and qualified nickel-iron alloy raw material can be obtained. According to the method, a characteristic of the alloy waste material that sulfiding volatilization of tin is easy to happen is taken full advantages of, extract effect of tin is obvious; no other impurity element is induced into the furnace hearth, and quality of the obtained nickel-iron alloy is high; operation of the technological process is simple, and popularization and application prospect is promising.

The invention belongs to the technical field of cesium solution purification, and particularly provides a method for achieving cesium adsorption separation through extraction resin. The method comprises the steps of t-BAMBP extraction resin preparation, a cesium solution adsorption process and a cesium desorption process. Firstly, macroporous resin, t-BAMBP and a diluent are mixed uniformly, stirring, filtering and drying are carried out, and t-BAMBP extraction resin is prepared; a cesium solution and the t-BAMBP extraction resin are put into a container, the alkalinity of the above cesium solution is adjusted to range from 0.5 mol / L to 1.2 mol / L, stirring and filtering are carried out, and cesium adsorbed t-BAMBP extraction resin is obtained; and a desorption agent is added into the cesium adsorbed t-BAMBP extraction resin, stirring and filtering are carried out, and a purified cesium solution is obtained. The method is simple; and when petroleum ether is selected as the diluent, and hydrochloric acid is selected as the desorption agent, the optimal extraction rate of the method on the cesium solution can reach 95.2%, the desorption rate can reach 95%, and effective separation of the cesium solution and other heteroions is achieved.

The invention provides a complex tracer agent for oil field high-temperature hypersalinity block interwell monitoring and the application thereof. The tracer agent comprises one or more of chlorinated hydroxylamine erbium, hydroxylamine gadolinium chloride, chlorinated hydroxylamine dysprosium, chlorinated hydroxylamine ytterbium and chlorinated hydroxylamine holmium, and the tracer agent is formed by a tracer element and a complexing agent in a complexing mode. The tracer element is selected from one or more of erbium, gadolinium, dysprosium, ytterbium and holmium, the complexing agent is hydroxylammonium chloride, and the molar ratio between the tracer element and the complexing agent is 1:8-1:9 and preferably is 1:8. The tracer agent can resist high temperature (250 DEGC-300 DEG C) and hypersalinity (5000mg / L-20000mg / L). The invention further provides a tracer method for conducting the oil field high-temperature hypersalinity block interwell monitoring by means of the tracer agent, and the method comprises the process of conducting separation and enrichment on the tracer agent in a taken sample.

The invention discloses a method for measuring the content of benzotriazole in transformer oil. The method comprises the following steps: preparing a standard solution; pretreating a sample by using lignin nano sol; carrying out surface enhanced Raman spectroscopy detection on the treated standard solution and the to-be-detected sample, and establishing a correction curve on the logarithm of the concentration of the benzotriazole by using the characteristic peak intensity of the benzotriazole at 1386cm <-1> in a surface enhanced Raman spectrogram of the standard solution, and calculating the content of benzotriazole in the to-be-detected sample according to the correction curve and the characteristic peak intensity at 1386cm <-1> in the surface enhanced Raman spectrum of the to-be-detected sample. The surface enhanced Raman spectrum detection technology is used for replacing traditional high performance liquid chromatography detection, the method is cleaner, more environmentally friendly and safer, and on-site detection can be achieved in combination with a portable Raman spectrometer while the detection sensitivity is guaranteed.

The invention belongs to the technical field of composite materials, and particularly relates to a zinc-aluminum hydrotalcite carbon nanotube composite adsorption material and a preparation method andapplication thereof. The preparation method comprises the following steps: adding carbon nanotubes into a three-neck round bottom flask filled with concentrated nitric acid and concentrated sulfuricacid, stirring and reacting in an ultrasonic water bath for 1.5h, cooling to room temperature, filtering by suction and washing, and drying to obtain an intermediate product named CNT-COOH; weighing zinc chloride and aluminum chloride hexahydrate in a beaker, adding deionized water, stirring to dissolve, adding the intermediate product obtained in the step 1), slowly dropping a 2 mol L<-1> NaOH solution at a certain speed, adjusting pH to 10, stirring for 2-5 hours, and carrying out hydrothermal reaction to obtain the target product, Zn / Al-LDH @ CNTs. The method has the advantages of low synthesis cost, simple synthesis method, energy conservation, environmental protection, large adsorption capacity, high adsorption speed, wide application and practical applicability.

The invention provides a three-in-one molecular imprinted column capable of separating clenbuterol, salbutamol and ractopamine at the same time. The molecular imprinted column contains an imprinted polymer capable of respectively identifying and separating three molecules, namely the clenbuterol, the salbutamol and the ractopamine. A method for preparing molecular imprinted microspheres is to perform suspension polymerization in a water phase system, and obtained molecular imprinted polymer particles can be directly packed in the column for use without being ground and sieved.

The invention discloses a magnetic extraction and enrichment method of an effective component in rheum officinale. A rheum officinale extracting solution is prepared from rheum officinale medicinal materials or a rheum officinale solid preparation according to the pharmacopeia, wherein the rheum officinale extracting solution, mesoporous ferroferric oxide nano-particles and an ionic liquid are prepared into enriched mixed liquor in a manner of adding water; each 1230mu L of enriched mixed liquor comprises the following components: 20mu L of rheum officinale extracting solution, 8-20mg of nano mesoporous ferroferric oxide, 0.2-0.6mg of ionic liquid, and the balance of water; the pH value is 9-13. The method comprises the following steps: loading the enriched mixed liquor into a centrifuge tube, whirling for 10-30 seconds on a vortex finder, approaching the bottom of the centrifuge tube by a magnet after standing; gathering the mesoporous ferroferric oxide nano-particles at the bottom of the centrifuge tube, and removing an upper water solution, so as to obtain enriched mesoporous ferroferric oxide nano-particles; eluting by a methanol solution containing acetic acid of which the mass fraction is 1-4%; collecting eluent and drying by distillation, so as to prepare enriched rheum officinale extractum, wherein the ionic liquid is brominated 1-dodecyl-3-methylimidazole, chloridized 1-dodecyl-3-methylimidazole, 1-butyl-3-methyl imidazolium tetrafluoroborate or 1-butyl-3-methyl imidazole polystyrene sulfonate, preferably chloridized 1-dodecyl-3-methylimidazole. The method disclosed by the invention is fast in speed, good in enrichment effect, and high in repeatability and reproducibility.

The invention relates to a method for separating germanium from a levextrel resin by adsorptive extraction. The technical scheme is as follows: the method comprises the following steps: uniformly mixing an AB-8 macroporous resin, N235 and an impregnant, stirring in a 50-70 DEG C water bath for 1-3 hours, and heating to 80 DEG C to dry off the impregnant, thereby obtaining an N235 levextrel resin; putting a germanium-containing solution, the N235 levextrel resin and tartaric acid in a container, regulating the pH value to about 2.0, oscillating and stirring for 80-100 minutes, and filtering to obtain a germanium-adsorbed N235 levextrel resin; and adding an eluting agent into the germanium-adsorbed N235 levextrel resin, oscillating and stirring for 80-100 minutes, filtering, and taking the filtrate. The method is simple, and has favorable separation and enrichment effects; the extraction rate can reach 89% above, and the eluting rate can reach 90% above; and it is proved that the N235 levextrel resin only adsorbs germanium, so the purity is high, and the germanium can be effectively separated from impurity ions.

The invention discloses a synthetic method and an application of a crystal violet molecularly imprinted micro-sphere. Crystal violet serves as a template molecule, MAA (methacrylic acid) serves as a functional monomer under the condition of acetonitrile solvents, EGDMA (ethylene glycol dimethyl acrylate) or DVB (divinylbenzene) serves as a crosslinking agent, polymerization reaction is performed by a thermostatic water-bath vibration precipitation polymerization method to synthesize a crystal violet molecularly imprinted polymer, the molecularly imprinted micro-sphere can effectively adsorb the crystal violet and has good specific identifiable characteristics, and better separation and enrichment effects are achieved by identification and adsorption of the template molecule. Based on design of integral synthetic steps, the influence of optimized crosslinking agents, stirring modes, the template molecule, the functional monomer, crosslinking agents and solvent dosage proportion on the performance of the micro-sphere is further summarized, the adsorption capacity of the polymer is as high as 39.12 micro-mol / g, an imprinted factor is 3.17, and the synthesized polymer has good adsorption identification capacity for a target molecule and has an excellent application prospect.

The invention relates to a composite material for selectively separating and enriching podophyllotoxin and a preparation method thereof. The preparation method comprises the following steps: adding podophyllotoxin as a template molecule, graphene oxide, modified hydroxy propyl cellulose, a functional monomer, a cross-linking agent and an initiator into a polymerization solvent; then reacting in an inert atmosphere to synthesize a molecularly imprinted polymer, and washing off the template molecule podophyllotoxin to obtain the composite material, wherein the modified hydroxy propyl cellulose is prepared by the following steps: under the catalytic action of triethylamine, modifying hydroxy propyl cellulose by using acryloyl chloride, so as to obtain methacryloyl-hydroxy propyl cellulose containing a carbon-carbon double bond. The composite material prepared by the invention has the advantages of stable selectivity, relatively high extraction efficiency, rapid detection and the like, and can realize selective separation and enrichment of podophyllotoxin in complex samples such as a water sample and the like.

The invention belongs to the technical field of microorganism detection, and discloses a rapid pathogenic microorganism detection method which comprises the following specific steps: preparing a detection probe and a capture probe, carrying out incubating to obtain a sandwich product, drawing a standard working curve, and detecting a to-be-detected sample. The detection probe comprises a nanogold@ nitrogen doped graphene quantum dot nano material, and a sulfydryl-containing silent region Raman signal molecule and a broad-spectrum recognition molecule which are modified on the surface of the nano material; and the capture probe is an immunomagnetic bead modified with an antibody. Raman detection of pathogenic microorganism is realized by combining the detection probe with immunomagnetic beads, namely a mechanism of specific recognition coupled with broad-spectrum recognition. The sensor construction and detection process do not need large instruments, operation is simple and rapid, andthe sensitivity is high.

The invention discloses a molybdenum ion imprinted amino-functionalization chitosan I-EDA-CS adsorbent. A preparation method comprises the following steps: (1) adding an ammonium molybdate solid into a mixed solution of water and ethanol, and stirring for reaction to obtain a colorless transparent liquid; (2) dropwise adding ethylenediamine, continuously stirring at normal temperature, adding epoxy chloropropane, stirring in backflow, adding a proper amount of biomass chitosan, stirring in backflow, washing and drying to obtain an intermediate product; and (3) removing molybdenum imprinted ions from the intermediate product by HCl, washing the intermediate product with distilled water till the product becomes neutral, and drying to obtain molybdenum ion imprinted amino-functionalization chitosan. The preparation method is simple and green and is free of pollution, and the ion imprinted amino-functionalization chitosan adsorbent is high in separation and enrichment efficiency and can be used for selectively adsorbing rhenium from a rhenium contained solution. The preparation method is free of energy consumption, has energy saving and environment protecting effect, and has large adsorbing capacity, wide applicability and practical applicability.

The invention discloses a heavy metal ion adsorbent for grating glycine to chloromethylated polystyrene, a preparation method and an application thereof, and belongs to the technical field of a solid-phase extraction agent. The technical solution of the invention is that: a heavy metal ion adsorbent for grafting glycine to chloromethylated polystyrene takes chloromethylated polystyrene and glycine as raw materials, and glycine is grafted on the chloromethylated polystyrene surface through one-step synthesis. The invention further discloses the preparation method of the heavy metal ion adsorbent for grating glycine to chloromethylated polystyrene and the application of taking the adsorbent as the solid-phase extraction agent to selectively separate and enrich trace Pb2+ from environmental samples. The heavy metal ion adsorbent is simple in a synthetic method, good in selectivity and high in sensitivity, nontoxic, easy to regenerate and recyclable.

The invention discloses a synthetic method and an application of a crystal violet molecularly imprinted micro-sphere. Crystal violet serves as a template molecule, MAA (methacrylic acid) serves as a functional monomer under the condition of acetonitrile solvents, EGDMA (ethylene glycol dimethyl acrylate) or DVB (divinylbenzene) serves as a crosslinking agent, polymerization reaction is performed by a thermostatic water-bath vibration precipitation polymerization method to synthesize a crystal violet molecularly imprinted polymer, the molecularly imprinted micro-sphere can effectively adsorb the crystal violet and has good specific identifiable characteristics, and better separation and enrichment effects are achieved by identification and adsorption of the template molecule. Based on design of integral synthetic steps, the influence of optimized crosslinking agents, stirring modes, the template molecule, the functional monomer, crosslinking agents and solvent dosage proportion on the performance of the micro-sphere is further summarized, the adsorption capacity of the polymer is as high as 39.12 micro-mol / g, an imprinted factor is 3.17, and the synthesized polymer has good adsorption identification capacity for a target molecule and has an excellent application prospect.

The invention relates to a method for efficiently separating and purifying multiresidue sulfonamide antibiotics in a biological sample. The method is characterized by comprising the following steps of: (1) preparing a molecular imprinted polymer, comprising the steps of burdening according to the mol ratio of template molecule to functional monomer to cross linking agent of being 1: (2-8): 25 and obtaining the molecular imprinted polymer with special identification function to the sulfonamide antibiotics by adopting a body polymerization method; (2) weighing certain amount of biological sample to be separated into a mortar, grinding the biological sample by the mortar, adding right amount of the molecular imprinted polymer, and further homogenating to obtain a homogenated sample, wherein the weight ratio of the biological sample to be separated to the molecular imprinted polymer is 2: (1-5), and (3) placing the homogenated sample in a solid phase extraction column, sequentially rinsing with 2.0-4.0ml of water, 2.0-4.0ml of 10wt% acetonitrile aqueous solution, and then eluting with 3.0-5.0ml of methanol / methanoic acid mixed solution; and separating the sulfonamide antibiotics from the biological sample to be separated by being instantly dissolved in the methanol / methanoic acid mixed solution. The method provided by the invention has wide selectivity and good separation and purification effect.

The invention relates to a phospho modified cellulose adsorbent, and a preparation method and application thereof. The technical scheme is as follows: the preparation method comprises the following steps: taking 1,3-dibromopropane and tributyl phosphite, carrying out reaction at 138-142 DEG C for 18-22 hours, and carrying out rotary evaporation at 115 DEG C under reduced pressure to obtain an intermediate product I; taking DMF (N,N-dimethylformamide), adding OCS and NaH in a nitrogen protective atmosphere, stirring in an ice water bath for 1 hour, removing the ice water bath, heating to 40-45 DEG C, reacting for 4 hours or so, adding the intermediate product I, mixing and stirring to react at room temperature for 24 hours, carrying out vacuum filtration, washing respectively with HCl and distilled water, and drying to obtain an intermediate product II; and in a nitrogen protective system, mixing the intermediate product II with ethanol, adding a KOH solution, stirring under reflux at 75 DEG C for 24-50 hours, carrying out vacuum filtration, washing respectively with HCl and distilled water to a neutral state, and drying. The adsorbent provided by the invention has high separation and enrichment efficiency, and can be used for selectively adsorbing scandium from a mixed rare earth ion solution.

The invention discloses an extraction separation device for lithium isotopes. The device comprises an extraction reaction container, temperature control equipment and gas blowing-in equipment; the extraction reaction container comprises a first cavity and a second cavity surrounding the peripheral side surface of the first cavity; the temperature control equipment is connected with the second cavity through a guide pipe and used for providing temperature control liquid and enabling the temperature control liquid to circulate between the second cavity and the temperature control equipment; anda guide pipe is connected with the bottom of the first cavity through the gas blowing-in equipment and is used for providing gas and blowing the gas into the first cavity through a gas distribution plate. The invention further discloses an extraction separation method for the lithium isotopes, the extraction separation device is applied, gas is introduced during extraction, and the extraction temperature is controlled. According to the extraction separation device and the separation method for the lithium isotopes, the abundance of 6Li single-stage separation can be effectively improved.

The invention discloses a method for separating and enriching lithium isotopes, which comprises the steps of: S1, preparing an organic phase for extraction: using a crown ether derivative represented by the following formula A or formula B as an extracting agent, mixing the extracting agent with hydrophobic ions The liquid and the diluent are mixed to obtain an extraction organic phase; S2, the extraction water phase is prepared: the water-soluble lithium salt is prepared into a lithium salt solution, which is used as the extraction water phase; S3, the extraction organic phase and the extraction water phase are fully mixed and extracted and then separated. phase, to obtain a loaded organic phase and a raffinate; S4, calcining the loaded organic phase at a temperature of 400°C to 1000°C for 30min-200min to obtain a slag phase; S5, to dissolve the slag phase with water, and perform solid-liquid separation to obtain 6 Li enrichment solution; S6, with 6 The Li enriched solution is used as the extraction water phase, and the above steps S3 to S5 are repeated. Based on the crown ether extraction system, the invention provides a brand-new process method without back-extraction operation, is a rapid superposition cross-flow multi-stage cascade separation method, and enriches the method for separating and enriching lithium isotopes.