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Molecular imprinted column capable of separating clenbuterol, salbutamol and ractopamine at same time

A technology of molecularly imprinted columns and ractopamine, applied in separation methods, solid adsorbent liquid separation, analysis materials, etc., to achieve good separation and enrichment effect and uniform particle size

Active Publication Date: 2017-05-10
北京市饲料监察所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this invention, the molecularly imprinted material of clenbuterol acts as a capture object for clenbuterol molecules in a sample, directly captures clenbuterol molecules in the sample to be tested and directly detects them by color development, without involving clenbuterol Separation and purification of Luo molecules

Method used

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  • Molecular imprinted column capable of separating clenbuterol, salbutamol and ractopamine at same time
  • Molecular imprinted column capable of separating clenbuterol, salbutamol and ractopamine at same time
  • Molecular imprinted column capable of separating clenbuterol, salbutamol and ractopamine at same time

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1 Preparation of Clenbuterol Molecularly Imprinted Polymer

[0056] 1. Weigh 2g of clenbuterol into 20mL of water, add 2mL of 0.1moL / L hydrochloric acid to fully dissolve it; use 1moL / L of NaOH to adjust the pH to above 12; extract three times with an equal volume of chloroform, and extract After dehydration with 3 g of anhydrous sodium sulfate, and rotary vacuum drying at 50°C, white or nearly white clenbuterol crystalline powder was obtained.

[0057] 2. Add 100mL of 4% polyvinyl alcohol 1788 into the four-neck flask, stir at 400r / min while blowing nitrogen for 10min.

[0058] 3. In another container, mix 0.8mL DEAEM, 5.0mL EGDMA, 10.0mL octanol, 0.5g clenbuterol from which the inhibitor has been removed, and ultrasonically mix for 20min, then add 120mg AIBN, 5mL chloroform and continue ultrasonication for 5min , Add ice cubes during sonication to keep the temperature low. Maintain stirring speed to ensure good monomer dispersion.

[0059] 4. Continuous nit...

Embodiment 2

[0064] Example 2 Preparation of Salbutamol Molecularly Imprinted Polymer

[0065] 1. Add 100mL of 4% polyvinyl alcohol 1788 (PVA 1788) into a four-neck flask, stir at 400r / min while blowing nitrogen for 10min.

[0066] 2. In another container, 0.8mL MAA, 5.0mL EGDMA, 10.0mL octanol, 0.3g albuterol, which have been removed from the polymerization inhibitor, were mixed ultrasonically for 20min, then added 120mg AIBN, 5mL chloroform and continued ultrasonication for 5min. Add ice cubes and keep cold. Maintain stirring speed to ensure good monomer dispersion.

[0067] 3. Continuous nitrogen flow, 70°C, react for 24h.

[0068] 4. After the polymerization reaction, the obtained polymer microspheres were filtered out, rinsed with water three times, poured into a 200mL beaker, and stirred with deionized water at 80°C for 60min.

[0069] 5. Wash three times with 50 mL of methanol and two times with 50 mL of acetone to remove organic matter such as swelling agent and residual organic...

Embodiment 3

[0072] Example 3 Preparation of Ractopamine Molecularly Imprinted Polymer

[0073] 1. Add 100mL of 4% polyvinyl alcohol 1788 (PVA 1788) into a four-neck flask, stir at 400r / min while blowing nitrogen for 10min.

[0074] 2. In another container, mix 0.8mL MAA, 5.0mL EGDMA, 10.0mL octanol, 0.6g ractopamine, which have been removed from the polymerization inhibitor, for 20 minutes by ultrasonication, then add 120mg AIBN, 5mL chloroform and continue ultrasonication for 5 minutes. Add ice cubes and keep cold. Maintain stirring speed to ensure good monomer dispersion.

[0075] 3. Continuous nitrogen flow, 70°C, react for 24h.

[0076] 4. After the polymerization reaction, the obtained polymer microspheres were filtered out, rinsed with water three times, poured into a 200mL beaker, and stirred with deionized water at 80°C for 60min.

[0077] 5. Wash three times with 50 mL of methanol and two times with 50 mL of acetone to remove organic matter such as swelling agent and residual ...

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Abstract

The invention provides a three-in-one molecular imprinted column capable of separating clenbuterol, salbutamol and ractopamine at the same time. The molecular imprinted column contains an imprinted polymer capable of respectively identifying and separating three molecules, namely the clenbuterol, the salbutamol and the ractopamine. A method for preparing molecular imprinted microspheres is to perform suspension polymerization in a water phase system, and obtained molecular imprinted polymer particles can be directly packed in the column for use without being ground and sieved.

Description

[0001] Technical field: [0002] The invention relates to a molecularly imprinted solid-phase extraction column, in particular to a three-in-one molecularly imprinted solid-phase extraction column capable of simultaneously separating clenbuterol, albuterol and ractopamine in animal products and feed. [0003] Background technique: [0004] "Clenbuterol" is a β2-receptor agonist drug. Using molecular imprinting technology to detect clenbuterol is to use the clenbuterol compound to be tested as a template molecule, use functional monomer molecules to combine with template molecules, and cross-linking agents and initiators to promote cross-linking between monomers, thereby Obtain imprinted compounds against specific template molecules. Currently, clenbuterol, ractopamine, and salbutamol are the three "leanbuterol" compounds that are illegally abused more seriously. [0005] Most of the molecularly imprinted columns currently used to detect clenbuterol can only detect one clenbut...

Claims

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Application Information

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IPC IPC(8): B01D15/20B01J20/26B01J20/28B01J20/30C07C213/10C07C215/60C07C215/68G01N1/40C08F222/14C08F220/06C08F220/34C08F2/18
CPCB01D15/20B01J20/264B01J20/28004B01J20/28019C07C213/10C08F2/18C08F222/14G01N1/405C07C215/68C07C215/60C08F220/34C08F220/06
Inventor 姚婷孙志伟刘钧魏秀莲王继彤杨宝良冯秀燕娄迎霞
Owner 北京市饲料监察所
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