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A magnetic extraction and enrichment method for effective components in rhubarb

A technology of active ingredients and rhubarb extract, which is applied to medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, pharmaceutical formulations, etc., can solve problems such as no reports in the field of separation and enrichment, and achieve superior separation. Enrichment performance, no pollution to the environment, and the effect of improving the enrichment effect

Active Publication Date: 2016-05-25
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, magnetic mesoporous materials have not been reported in the field of separation and enrichment of traditional Chinese medicine and natural products.

Method used

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  • A magnetic extraction and enrichment method for effective components in rhubarb
  • A magnetic extraction and enrichment method for effective components in rhubarb
  • A magnetic extraction and enrichment method for effective components in rhubarb

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1. Investigate the impact of the type of ionic liquid on the enrichment effect

[0044] 1.1 Take 4 clean 1.5mL centrifuge tubes, numbered 1, 2, 3, and 4. Add 100 μL of mesoporous ferric oxide nano-dispersion (80 mg / mL), 1100 μL of water with pH=11, 20 μL of rhubarb extract.

[0045] 1.2 Add 1-dodecyl-3-methylimidazole bromide aqueous solution (40 mg / mL) 10 μL to No. 1 centrifuge tube, and add 1-dodecyl-3-methylimidazole chloride to No. 2 centrifuge tube Aqueous solution (40mg / mL) 10μL, add 1-butyl-3-methylimidazolium tetrafluoroborate aqueous solution (40mg / mL) 10μL to No. 3 centrifuge tube, add 1-butyl-3- Methylimidazole polystyrene sulfonate aqueous solution (40mg / mL) 10μL.

[0046] 1.3 Place the 4 centrifuge tubes together on the vortex instrument for 10 seconds and let them stand for 5 minutes.

[0047] 1.4 Use a magnet close to the bottom of the centrifuge tube to gather mesoporous ferric oxide nanoparticles at the bottom of the centrifuge tube, and disc...

Embodiment 2

[0061] Example 2. Investigate the impact of different ionic liquid addition volumes on the enrichment effect

[0062] 2.1 Take 4 clean 1.5mL centrifuge tubes, numbered 1, 2, 3, and 4. Add 100 μL of mesoporous ferric oxide nano-dispersion (80 mg / mL) and 20 μL of rhubarb extract to 1-4.

[0063] 2.2 Add 10 μL of pure water and 1100 μL of water with pH=11 to No. 1 centrifuge tube, add 5 μL of 1-dodecyl-3-methylimidazole chloride aqueous solution (40 mg / mL) and pH=11 to No. 2 centrifuge tube 1105 μL of water, 10 μL of 1-dodecyl-3-methylimidazole chloride aqueous solution (40 mg / mL) and 1100 μL of water with pH=11 were added to No. 3 centrifuge tube, and 1-10 Dialkyl-3-methylimidazole aqueous solution (40 mg / mL) 15 μL and water with pH=11 1095 μL.

[0064] 2.3 Place the four centrifuge tubes together on a vortex instrument for 10 seconds and let them stand for 5 minutes.

[0065] 2.4 Use a magnet close to the bottom of the centrifuge tube to gather the mesoporous Fe3O4 nanopartic...

Embodiment 3

[0073] Example 3. Investigate the influence of different pH values ​​on the enrichment effect

[0074] 3.1 Take 4 clean 1.5mL centrifuge tubes, numbered 1, 2, 3, 4. Add 100 μL of mesoporous ferric oxide nano-dispersion (80 mg / mL) and 1-dodecane chloride to 1-4. Base-3-methylimidazole aqueous solution (40mg / mL) 10μL, rhubarb extract 20μL.

[0075] 3.2 Add 1100 μL of pH=5 water to No. 1 centrifuge tube, add 1100 μL of pH=7 water to No. 2 centrifuge tube, add 1100 μL of pH=9 water to No. 3 centrifuge tube, add pH=11 to No. 4 centrifuge tube Add 1100 μL of water with pH=13 to No. 5 centrifuge tube.

[0076] 3.3 Place the 4 centrifuge tubes together on the vortex instrument for 10 seconds and let them stand for 5 minutes.

[0077]3.4 Use a magnet close to the bottom of the centrifuge tube to gather the mesoporous Fe3O4 nanoparticles at the bottom of the centrifuge tube, and discard the upper aqueous solution.

[0078] 3.5 Use methanol solution containing 2% acetic acid to elute ...

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PUM

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Abstract

The invention discloses a magnetic extraction and enrichment method of an effective component in rheum officinale. A rheum officinale extracting solution is prepared from rheum officinale medicinal materials or a rheum officinale solid preparation according to the pharmacopeia, wherein the rheum officinale extracting solution, mesoporous ferroferric oxide nano-particles and an ionic liquid are prepared into enriched mixed liquor in a manner of adding water; each 1230mu L of enriched mixed liquor comprises the following components: 20mu L of rheum officinale extracting solution, 8-20mg of nano mesoporous ferroferric oxide, 0.2-0.6mg of ionic liquid, and the balance of water; the pH value is 9-13. The method comprises the following steps: loading the enriched mixed liquor into a centrifuge tube, whirling for 10-30 seconds on a vortex finder, approaching the bottom of the centrifuge tube by a magnet after standing; gathering the mesoporous ferroferric oxide nano-particles at the bottom of the centrifuge tube, and removing an upper water solution, so as to obtain enriched mesoporous ferroferric oxide nano-particles; eluting by a methanol solution containing acetic acid of which the mass fraction is 1-4%; collecting eluent and drying by distillation, so as to prepare enriched rheum officinale extractum, wherein the ionic liquid is brominated 1-dodecyl-3-methylimidazole, chloridized 1-dodecyl-3-methylimidazole, 1-butyl-3-methyl imidazolium tetrafluoroborate or 1-butyl-3-methyl imidazole polystyrene sulfonate, preferably chloridized 1-dodecyl-3-methylimidazole. The method disclosed by the invention is fast in speed, good in enrichment effect, and high in repeatability and reproducibility.

Description

technical field [0001] The invention belongs to the field of extraction and preparation of natural medicines. It relates to a method for extracting and enriching traditional Chinese medicine, specifically, it relates to a new method for extracting and enriching active ingredients in rhubarb traditional Chinese medicine by wrapping mesoporous Fe3O4 nanometer material with ionic liquid as an adsorbent. Background technique [0002] Rhubarb is a representative of traditional Chinese medicines for purging. It is a commonly used traditional Chinese medicine recorded in previous editions of "Chinese Pharmacopoeia" and physicians' materia medica. The effects of purging and attacking stagnation, expelling blood stasis and dredging menstrual flow, promoting dampness and reducing jaundice, clearing away heat and purging fire, cooling blood and detoxifying. Rhubarb contains a variety of active ingredients, among which anthraquinones, dianthrones, stilbenes, tannins and polysaccharides...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/708
Inventor 曹君胡帅帅代菡镔刘晓娟达建华曹婉
Owner HANGZHOU NORMAL UNIVERSITY
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