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Rapid aflatoxin B1 detecting method based on smartphone detecting system

A kind of aflatoxin and smart phone technology, which is applied in the preparation of test samples, measuring devices, and material analysis through optical means, which can solve the problems of not being able to directly display the concentration value of the test substance, and shorten the test time and cost. Inexpensive and accurate results

Active Publication Date: 2016-07-20
TAIYUAN UNIV OF TECH
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Problems solved by technology

And Wong et al. (J.X.Wong, F.S.LiuandH-ZYu, Mobileapp-basedquantitativescanometricalysis, Anal.Chem., 2014, 86, 11966-11971) have studied the purpose of quantitative detection by analyzing the optical gray scale ratio through the mobile phone application, using biotin React with streptavidin to verify its performance. Due to the limitation of the number of channels, it has certain limitations in multi-throughput detection and it only gives the optical gray scale ratio and cannot directly display the concentration value of the test substance.

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  • Rapid aflatoxin B1 detecting method based on smartphone detecting system
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  • Rapid aflatoxin B1 detecting method based on smartphone detecting system

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Embodiment Construction

[0038] The specific embodiment of the present invention will be further described in detail below by taking cereals as an example.

[0039] as attached figure 1 to attach Figure 5 As shown, the specific steps for implementing the aflatoxin B1 rapid detection method based on the smart phone detection system provided above in the present invention are as follows.

[0040] (1) Preparation of aflatoxin B1 microchannel: Cut the transparent plastic sheet into a square of 5.5cm×6cm as the transparent base layer, put it into alcohol for ultrasonic cleaning for 10 minutes, and then put it into UV / Ozone for treatment 15 minutes incubation for 20 minutes. Dissolve 0.36g of EDC and 0.045g of NHS in 0.1M phosphate buffer (pH=6) as an activation solution. Immerse the transparent substrate in the activation solution for 5 hours to activate. After taking it out, wash it with ultrapure water and dry it with nitrogen. Sylgard 184 silicone rubber from Dow Corning Company was selected to prep...

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Abstract

The invention discloses a rapid aflatoxin B1 detecting method based on a smartphone detecting system.The rapid aflatoxin B1 detecting method includes the steps that aflatoxin B1 microchannels are prepared, detection reagents are added into the microchannels to prepare silver-stained PC sheet, aflatoxin-B1-detection optical hardware is designed through 3DMax software and printed through a 3D printer, the silver-stained PC sheet and a mobile phone are arranged into an optical hardware device, an aflatoxin B1 reaction area is photographed through a camera of the mobile phone, and the detection result of aflatoxin B1 is processed and displayed through a smartphone detection program.According to the rapid aflatoxin B1 detecting method, aflatoxin B1 is rapidly detected based on a smartphone, and the rapid aflatoxin B1 detecting method has the advantages of being convenient to carry, easy and convenient to operate, low in cost, high in flexibility and the like, and is suitable for rapid site detection.

Description

technical field [0001] The invention relates to a rapid detection method for aflatoxin B1, specifically a method for adding aflatoxin B1 detection solution to polycarbonate (PC) and polydimethylsiloxane (PDMS) as materials In the multi-flux microchannel of the company, the silver-stained transparent plastic sheet is inserted into the hardware device, and the optical gray ratio (ODR) of the reaction band is calculated through the smart phone detection program to realize the analysis method of rapid quantitative detection of aflatoxin B1 . Background technique [0002] As the food industry enters a new stage, food safety issues have attracted worldwide attention. Among the many food safety risk factors, mycotoxin contamination is the most serious, among which aflatoxin B1 is the most toxic and carcinogenic. Traditional detection methods are mainly based on expensive large-scale instruments, such as high performance liquid chromatography, liquid chromatography-mass spectromet...

Claims

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Application Information

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IPC IPC(8): G01N21/84G01N1/28G01N1/38H04M1/725H04M1/72403
CPCG01N1/28G01N1/38G01N21/84H04M1/72403
Inventor 李晓春杨帆张校亮于化忠
Owner TAIYUAN UNIV OF TECH
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