Preparation of quantitative rapid detecting sensor of microcapsule algae toxin and applications

A technology for detecting sensors and microcystins, applied in the biological field, can solve the problems of time-consuming and laborious, unable to monitor real-time source water, etc., and achieve the effect of sensitive detection

Inactive Publication Date: 2009-06-17
上海雄图生物科技有限公司
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Problems solved by technology

However, these methods are time-consuming and laborious, have

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  • Preparation of quantitative rapid detecting sensor of microcapsule algae toxin and applications
  • Preparation of quantitative rapid detecting sensor of microcapsule algae toxin and applications

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[0029] Example 1: Quantitative and rapid detection of microcystin-LR in natural lake water:

[0030] 1 Pre-treatment:

[0031] Sampling is carried out by a plexiglass deep-water sampler, the depth of water collection is less than 0.5m, and the sampling volume is 5L. Large particles of impurities are removed through a sand core funnel, and then filtered through a 0.45μm filter membrane under reduced pressure. Collect the filtrate. Take an appropriate amount of filtrate for detection and analysis.

[0032] 2 How to use the microcystin-LR quantitative rapid detection sensor:

[0033] Take out the prepared MC-LR immunoelectrode sensor and equilibrate to room temperature. According to the order of concentration from low to high, 10μL of MC-LR standard solution and sample extract of different concentrations were added dropwise to the surface of the electrode, and incubated at 37°C for 15 minutes, then washed with PBS and dried. Using cyclic voltammetry (CV), alternating current impedanc...

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Abstract

The invention provides preparation and application of a quantitative and rapid detection biosensor of microcystic toxin-LR, and belongs to the biotechnology field. The detection sensor is an impedance type electrochemical sensor, and consists of a CHI760C type electrochemical work station and a three-electrode system in which an immunity electrode is taken as a working electrode, a saturated calomel electrode is taken as a reference electrode and a platinum wire electrode is taken as a counter electrode; L-cysteine, colloidal gold and microcystic toxin antibody compound are respectively enveloped on a surface of a bare mitallic electrode to be taken as the working electrode; a change of electric potential difference between the reference electrode and the working electrode takes place when the microcystic toxin antibody is combined with the antigen (MC-LR) in sample solution, and is subject to a quantitative relation with the concentration of the antigen in the sample, then the detection of the microcystic toxin is carried out; the biosensor for rapidly, specifically and sensitively detecting microcystic toxin antigens is successfully established; the detection linear range is 0.05-300ng/mL, and the detection limit is 1.82*10<-2>ng/mL. The biosensor can perform real-time monitoring on natural lake water, and can also perform real-time monitoring on preparation process of drinking water in water supply works.

Description

technical field [0001] The preparation and application of a microcystin-LR quantitative rapid detection sensor belongs to the field of biotechnology. Background technique [0002] Microcystins (MC for short) have become one of the pollutants that are widely concerned in environmental water. MC is a kind of biologically active cyclic heptapeptide compound, which is mainly produced by the freshwater alga Microcystis aeruginosa. So far, more than 70 isomers of MC have been found. It is a highly specific toxin with the liver as the target organ, and has obvious hepatotoxicity. With the in-depth study of MC toxicity, it is found that MC has multi-organ toxicity, hereditary and carcinogenicity. The structure of algal toxins is mainly composed of N-methyl dehydroalanine and two L-amino acid residues X and Z. Common three toxins are MC-LR, RR, and YR (L, R, and Y represent leucine respectively Acid, arginine, tyrosine), and MC-LR is the main toxin in fresh water. Algae toxin is a ...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N33/543
Inventor 孙秀兰张银志石慧肖理文李鲁宁许欣徐丹
Owner 上海雄图生物科技有限公司
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