4 results about "L929 cell" patented technology

The invention discloses a gel microneedle loaded with silver nanoparticles and a preparation method and application thereof.The extract nano-silver (AB-AgNPs) is prepared through a green synthesis strategy, a drug-loaded microneedle system is constructed based on carboxymethyl chitosan-vanillic aldehyde-polyvinyl alcohol (CMCS-Va-PVA) composite gel, the AB-AgNPs prepared through the technology are uniform in particle size and stable in dispersion, and the drug-loaded microneedle has the advantages that the drug-loaded microneedle is good in drug-loaded performance and good in drug-loaded performance; the crystal has a typical face-centered cubic crystal structure and metal state Ag0 characteristics; the gel microneedle has good mechanical strength and forming effect, the AB-AgNPs and the gel matrix are stably combined through hydrogen bonds, the chemical properties are kept unchanged, and the system has broad-spectrum efficient bacteriostatic activity and biocompatibility; an in-vitro experiment proves that the green synthesized gel microneedle loaded with the silver nanoparticles has a remarkable capability of promoting the healing of L929 cell tissues; a rat wound experiment proves that the gel microneedle can regulate and control an inflammatory factor network and promote cell migration and tissue regeneration, healing of common and infected wounds is remarkably accelerated, and the wound residue ratio in 9 days is as low as 2.7% and 10.9% respectively.

This application belongs to the field of biomaterials, specifically relating to an antibacterial polymeric derivative of F127 with terminal modification of acetyl bromide, its preparation method, and its application. This application obtains F127AcBr by terminal modification of polymeric F127 with acetyl bromide via an acylation reaction. Its good antibacterial effect and biocompatibility were verified in vitro using plate antibacterial assays against Staphylococcus aureus and Escherichia coli, as well as live / dead staining assays of L929 cells and HUVEC cells. This application optimized the concentration of F127AcBr and loaded it into an MS hydrogel system. Excellent biocompatibility was confirmed in a diabetic mouse wound model using HE staining of mouse organs. Immunofluorescence staining against Staphylococcus aureus further confirmed the good antibacterial activity of F127AcBr.

This invention belongs to the field of protein technology and relates to a peptide that prevents collagen loss and its preparation method. The amino acid sequence of the peptide is KSYELPDGQVITIG, with the key active fragment being ELPDGQVIT. The peptide is obtained by enzymatic hydrolysis of defatted grain insect powder using alkaline protease, trypsin, papain, neutral protease, and flavor protease. This peptide can inhibit the expression of MMP1 and MMP9 in L929 cells after UVA irradiation, thus alleviating collagen loss.

The invention discloses modified hyaluronic acid as well as a modification process and application thereof, and particularly relates to a method for chemically modifying hyaluronic acid by taking glutathione (GSH) as a functional molecule on the basis of a modification strategy of succinic anhydride bridged acylation on hyaluronic acid: under an alkaline condition, after ring opening of succinic anhydride, forming an ester bond with hydroxyl of hyaluronic acid, so as to obtain the modified hyaluronic acid. And at the same time, the other carboxyl group is condensed with the amino group of GSH to generate an amido bond, and the modified hyaluronic acid with a three-dimensional network structure is constructed. Performance tests show that the glutathione grafting rate of the obtained modified hyaluronic acid is up to 16.4%, the oxidation resistance is remarkably superior to that of unmodified hyaluronic acid, the enzymolysis resistance is reduced from 11.98% to 5.12%, the original moisturizing performance is not influenced, and the modified hyaluronic acid has no remarkable toxicity to L929 cells. According to the process provided by the invention, the oxidation resistance and enzyme stability of HA are effectively improved, and a new way is provided for application of HA in the field of anti-aging skin care.