Embryo cattle serum substitute without serum for animal cell culture

Abstract

The invention relates to a non-serum animal cell culture used fetus cattle serum displace agent, which is formed by recombination human insulin growth factor-1:0.1g-0.5g / l, recombination human platelet sourced growth factor-B:0.1g-0.6g / l, recombination human alkali desmocyte growth factor: 0.05-0.4g / l, cattle transferring: 1g-5g / l, cattle serum protein (V component): 2g-15g / l, and mercaptoethanol 0.3-0.5mol / l.

Description

technical field [0001] The invention relates to a serum-free formula fetal bovine serum substitute for animal cell culture, in particular to a fetal bovine serum substitute (serum-free formula) for animal cell culture that can be matched with various basal media. Background technique [0002] The in vitro culture of animal cells mostly uses serum-containing medium for culture, which is costly, the serum composition is not clear, and it is not easy to purify downstream products. More importantly, the serum contains potential viruses (such as mad cow virus, etc.), prion or mycoplasma contamination The biological effects of serum supporting cell growth have been proven, but the complex components in serum have not yet been fully understood. There are not only cell growth-promoting factors in serum, but also cell growth-inhibiting factors or toxic factors in the same pair, so The biological characteristics reflected by cells cultured in serum-containing medium are the comprehens...

AI Technical Summary

Problems solved by technology

[0013] Studies have shown that cells can be stably passaged in serum-free medium for a long time. However, the serum-free medium currently studied mainly shows that the applicable spectrum of cells is extremely narrow, and cells are more susceptible to certain mechanical and chemical factors in serum-free medium. Influence, storage and application of medium are not as convenient as traditional synthetic medium