Embryo cattle serum substitute without serum or animal protein for animal cell culture

Abstract

The invention relates to a non-serum non-animal origin protein animal cell culture used fetus cattle serum displace agent, which is formed by recombination human insulin growth factor-1:0.1g-0.5g / l, recombination human platelet sourced growth factor-B:0.1g-0.6g / l, recombination human alkali desmocyte growth factor: 0.05-0.4g / l, polyethylene glycol (MW7000-20000), 0.04-0.15%(w / v), polyvinyl alcohol (density 1.27-1.31): 0.001-0.05%(w / v), mercaptoethanol: 0.3-0.5mol / l.

Description

technical field [0001] The invention relates to a fetal bovine serum substitute for animal cell serum-free culture, in particular to a fetal bovine serum substitute for animal cell culture with serum-free and animal-derived protein formula. Background technique [0002] The in vitro culture of animal cells mostly uses serum-containing medium for culture, which is costly, the serum composition is not clear, and it is not easy to purify downstream products. More importantly, the serum contains potential viruses (such as mad cow virus, etc.), prion or mycoplasma contamination and other shortcomings. The culture medium formulated by replacing serum with serum substitutes completely adopts the culture medium with known molecular structure and configuration components. Purification, and avoid problems such as potential toxicity in serum. [0003] At present, there are commercial serum-free media for CHO cell culture abroad, and domestic scholars have developed a series of serum-...

AI Technical Summary

Problems solved by technology

[0005] Kattinger H et al. (Advances Mol.Cell.Biology, 1996, 15A, 193-207) have studied that cells can grow for a long time in protein-free medium, but these cells must be cultured on carriers, and cells cannot be cultured continuously

[Chinese patent application number: 00816020.1] researched and invented a protein-free medium with a certain proportion of soybean hydrolyzate as the main ingredient. It can basically achieve protein-free and serum-free culture of animal cells, but its operation is more complicated and complicated. Still contains a large number of unknown plant components