Embryo cattle serum substitute without serum or animal protein for animal cell culture

A technology of animal cell culture and fetal bovine serum, which is applied in the field of fetal bovine serum substitutes for animal cell culture, can solve the problems of complicated operation and continuous cell culture

Inactive Publication Date: 2006-07-12
上海席诺生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Kattinger H et al. (Advances Mol.Cell.Biology, 1996, 15A, 193-207) have studied that cells can grow for a long time in protein-free medium, but these cells must be cultured on carriers, and cells cannot be cultured continuously
[Chinese patent application number: 00816020.1] researched and

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The present invention consists of recombinant human insulin-like growth factor-1: 0.1g / L, recombinant human platelet-derived growth factor-B: 0.6g / L, recombinant human basic fibroblast growth factor: 0.4g / L, polyethylene glycol Alcohol (MW7000~20000) 0.04% (w / v), current polyvinyl alcohol (density 1.27~1.31): 0.05% (w / v), mercaptoethanol: 0.3mol / L.

Embodiment 2

[0015] The present invention consists of recombinant human insulin-like growth factor-1: 0.3g / L (concentration), recombinant human platelet-derived growth factor-B: 0.4g / L, recombinant human basic fibroblast growth factor: 0.05g / L, Polyethylene glycol (MW7000~20000), 0.15% (w / v), current polyvinyl alcohol (density 1.27~1.31): 0.001% (w / v), mercaptoethanol: 0.5mol / L.

Embodiment 3

[0017] The present invention consists of recombinant human insulin-like growth factor-1: 0.5g / L (concentration), recombinant human platelet-derived growth factor-B: 0.35g / L, recombinant human basic fibroblast growth factor: 0.25g / L, Polyethylene glycol (MW7000-20000), 0.1% (w / v), existing polyvinyl alcohol (density 1.27-1.31): 0.02% (w / v), mercaptoethanol: 0.4mol / L.

[0018] Preparation method

[0019] First, prepare a basic medium consistent with that used for cell culture, such as RPMI1640 or DMEM or F12 or DMEM / F12, etc., the prepared concentration is 4 times the normal concentration, and sterilize and filter with a 0.2 μm filter membrane;

[0020] Dissolve the weighed polyethylene glycol into 20 times the mass of ultrapure water, and filter with a 0.45 μm filter membrane;

[0021] Mix under slow stirring, add mercaptoethanol in proportion, then add the calculated volumes of recombinant human insulin-like growth factor-1, recombinant human platelet-derived growth factor-B ...

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PUM

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Abstract

The invention relates to a non-serum non-animal origin protein animal cell culture used fetus cattle serum displace agent, which is formed by recombination human insulin growth factor-1:0.1g-0.5g/l, recombination human platelet sourced growth factor-B:0.1g-0.6g/l, recombination human alkali desmocyte growth factor: 0.05-0.4g/l, polyethylene glycol (MW7000-20000), 0.04-0.15%(w/v), polyvinyl alcohol (density 1.27-1.31): 0.001-0.05%(w/v), mercaptoethanol: 0.3-0.5mol/l.

Description

technical field [0001] The invention relates to a fetal bovine serum substitute for animal cell serum-free culture, in particular to a fetal bovine serum substitute for animal cell culture with serum-free and animal-derived protein formula. Background technique [0002] The in vitro culture of animal cells mostly uses serum-containing medium for culture, which is costly, the serum composition is not clear, and it is not easy to purify downstream products. More importantly, the serum contains potential viruses (such as mad cow virus, etc.), prion or mycoplasma contamination and other shortcomings. The culture medium formulated by replacing serum with serum substitutes completely adopts the culture medium with known molecular structure and configuration components. Purification, and avoid problems such as potential toxicity in serum. [0003] At present, there are commercial serum-free media for CHO cell culture abroad, and domestic scholars have developed a series of serum-...

Claims

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Application Information

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IPC IPC(8): C12N5/06C12N5/07
Inventor 朱文洲
Owner 上海席诺生物技术有限公司
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