A new natural product polysaccharide cis-a from Mushroom magnificence and its application
A technology of CIS-A and natural products, which is applied in drug combinations, allergic diseases, antineoplastic drugs, etc., can solve the problems that have not been reported before, and achieve the goal of improving the ability, improving the ability to swallow neutral red, and promoting proliferation Effect
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[0032] Example 1 Extraction, Separation and Purification of Polysaccharide CIS-A
[0033] 1.1. Extraction, separation and purification of polysaccharide CIS-A from Mushroom magnificence
[0034] 1.1.1 Extraction of crude polysaccharides from Mushroom magnifolia
[0035] Place the fruiting body of Mushroom magnificence in a constant temperature box at 65°C to dry, then crush it completely with a pulverizer, weigh 300g of the crushed powder, add distilled water and stir evenly (1:3), cook at 110°C for 6 hours, and centrifuge Collect the supernatant, repeat the cooking three times, and concentrate all the supernatant under reduced pressure, then add 3-4 times the volume of absolute ethanol to precipitate the crude polysaccharides of the Magnificent Pleurotus chinensis. The precipitate was dried and stored in a -20°C refrigerator.
[0036] 1.1.2 Separation and purification of crude polysaccharides from Mushroom magnifolia
[0037] Weigh 50g DEAE-cellulose, soak overnight in dis...
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