Odorranagrahami antimicrobialpeptides and application thereof

A technology of antibacterial peptides and stinky frogs, which is applied in the field of biomedicine and can solve the problems of insufficient research on skin active peptides

Inactive Publication Date: 2009-08-05
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The application of amphibian drugs has a long history in my country, but the research on its active ingredients and pharmacological properties mainly focuses on small organic molecules such as alkaloids, and there are not many studies on its skin active peptides

Method used

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  • Odorranagrahami antimicrobialpeptides and application thereof
  • Odorranagrahami antimicrobialpeptides and application thereof
  • Odorranagrahami antimicrobialpeptides and application thereof

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Embodiment Construction

[0014] The substantive content of the present invention is further described below with embodiment, but content of the present invention is not limited thereto.

[0015] Preparation of antimicrobial peptides from the fingerless frog:

[0016] Clean the living fingerless stink frog with water, put it in a glass container with a cover, add anhydrous ether (1 liter of volume plus 1 ml of anhydrous ether) dropwise, and seal the container for 3-5 minutes, and a large amount of foam can be seen. Substances are secreted from the skin of the frog. The secretions are collected, centrifuged (10000rpm, 20 minutes), freeze-dried and stored.

[0017] In the first step, Sephadex G-50 gel filtration column chromatography, 300 mg of the lyophilized powder of the skin secretion of the stink frog skin secretion obtained by the above method was dissolved in 5 ml of purified water, and centrifuged at 10,000 rpm at 4°C for 10 min to remove the precipitate. Sephadex G-50 gel filtration column (len...

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Abstract

The present invention relates to rana grahami antibiotic peptide and its application, and belongs to the field of biomedicine. Rana grahami antibiotic peptide is one kind of cyclic polypeptide separated from the secretion of amphibian rana grahami, and has molecular weight of 1705.3 dalton, isoelectric point of 9.39, and primary structure of polypeptide sequence Leu Lys Gly Cys Trp The Lys Ser Ile Pro Pro Lys Pro Cys Phe(LKGCWTKSIPPKPCF) with cysteines in the 4-th and 14-th forming intramolecular disulfide bond. Its preparation process includes collecting secretion of ether stimulated rana grahami, centrifuging to eliminate precipitate, freeze drying, chromatographic separation in filtering gel column and separating purification in reverse high pressure liquid chromatogram. The rana grahami antibiotic peptide has powerful effect of inhibiting bacteria, fungi and viruses and no hemolytic activity, and may be used in preparing medicine for treating pathogenic microbe infection diseases.

Description

Technical field: [0001] The invention relates to a Rana grahami antibacterial peptide, which belongs to the field of biomedicine. Background technique: [0002] The discovery of penicillin made people no longer helpless against various diseases caused by pathogenic microorganism infection, and thus developed a large number of β-lactam antibiotics, which made a great contribution to the protection of human health. However, with the widespread use of the above-mentioned "traditional antibiotics", many new problems continue to arise. For example, allergic reactions to β-lactam antibiotics and long-term use lead to the production of drug-resistant strains. So people began to look for a new generation of antibacterial agents. Recent studies have found that peptide antibiotics have broad-spectrum antibacterial activity, and at the same time have incomparable advantages over "traditional antibiotics": such as rapid and broad-spectrum killing of microorganisms (including current c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08A61K38/10A61P31/00
Inventor 赖仞李建许徐学清徐春花李东升吕毅
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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