Novel antioxidant active polypeptide OA-VI12 and preparation method and application thereof

A technology of OA-VI12, antioxidant activity, applied in the field of biomedicine, can solve problems such as insufficient abundance, and achieve the effects of high antioxidant activity, great medical application prospects, and natural sources

Inactive Publication Date: 2019-12-20
KUNMING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Non-genetically encoded low-molecular-weight antioxidants, such as vitamin C, play an important role in protecting the internal structure and water content of cells; high-molecular-weight gene-encoded enzymes, such as superoxide dismutase, are defense systems that protect mitochondria and tissues; however , small molecular peptides are considered to have broad development prospects due to their low energy consumption, high speed, high efficiency and little awareness of them at present
The currently known antioxidant peptides in peptides encoded by small molecule genes mainly include AOP-P1 from Yunnan frog and RL from the big green stink frog, but they are still far from enough compared to the richness of their sources.

Method used

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  • Novel antioxidant active polypeptide OA-VI12 and preparation method and application thereof
  • Novel antioxidant active polypeptide OA-VI12 and preparation method and application thereof
  • Novel antioxidant active polypeptide OA-VI12 and preparation method and application thereof

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preparation example Construction

[0025] The preparation method of the novel antioxidant active polypeptide OA-VI12 of the present invention comprises the following steps:

[0026] A. Take the skin secretion of the Yunnan stink frog by electrical stimulation, dissolve it in Tris-HCl, freeze-dry it in vacuum to obtain material a, and store it at -80°C for later use;

[0027] B. Dissolving material a in water to obtain material b, material b is eluted and fractionated to obtain sample c;

[0028] C. The sample c is subjected to high-performance liquid chromatography reversed-phase chromatography to obtain the target novel antioxidant activity polypeptide OA-VI12.

[0029] The water described in the B step is deionized water.

[0030] The elution described in step B was eluted on a Sephadex G-75 gel filtration column with TriS-HCl (pH 7.8) mixed with NaCl as the elution buffer.

[0031] The fractionation described in step B is to use an automatic split collector to collect fractions every 8-12 minutes, and the ...

Embodiment 1

[0036] 1. Sample Collection and Animal Care

[0037] Adult yunnanensis frogs were collected from Lijiang City, Yunnan Province and transported safely to the laboratory. Stink frogs were collectively housed in a 50 cm x 60 cm container with mealworms ready for consumption by the frogs. After acclimating to the environment for a week, the frog skin was stimulated with an electric massager with 6 volts alternating current. Then the skin secretions of the stink frog were washed with 25 mM Tis-HCl buffer (pH 7.8). The obtained secretion was centrifuged at 4000 g (4 degrees) for 15 min, and the supernatant was collected and lyophilized. All samples were stored at -80°C until further analysis.

[0038] 2. Purification procedure

[0039] Lyophilized skin secretion samples (500 μL, OD280 = 50) were dissolved in deionized water and applied to a Sephadex G-75 gel filtration column, eluted with 25 mM TriS-HCl (pH 7.8) mixed with 0.1 M NaCl The buffer was eluted at a flow rate of 0.1 ...

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Abstract

The invention discloses a novel antioxidant active polypeptide OA-VI12 and a preparation method and an application thereof. An amino acid sequence of the novel antioxidant active polypeptide OA-VI12 is VIPFLACRPLGL. The antioxidant active polypeptide is extracted from skin secretions of rana andersonli, and the polypeptide has the characteristics of natural source, high activity and the like. Thenovel antioxidant active polypeptide OA-VI12 obtained by purification shows the relatively high stability and antioxidant activity and plays a beneficial role in protecting cells from UV irradiation and oxidative stress caused by hydrogen peroxide stimulation, and more importantly, the novel antioxidant active polypeptide OA-VI12 also achieves the effect of protecting the skin from photodamage caused by ultraviolet radiation, so that it is indicated that the novel antioxidant active polypeptide OA-VI12 has a relatively wide medical application prospect. The novel antioxidant active polypeptideOA-VI12 has the beneficial characteristic of being capable of serving as an anti-ultraviolet drug candidate.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a novel antioxidant active polypeptide OA-VI12 and its preparation method and application. Background technique [0002] Among the factors related to the skin aging damage process, genetic and physiological factors account for 3%, and environmental factors account for 97%. Among the many environmental factors, solar radiation is one of the important causes of photoaging damage. There are three kinds of ultraviolet rays in sunlight: long-wave ultraviolet UVA (315-400 nanometers), medium-wave ultraviolet UVB (280-315 nanometers) and short-wave ultraviolet UVC (100-280 nanometers). Almost all UVC is absorbed by the ozone layer, and the biological activity of UVB is higher than that of UVA, so UVB is the main cause of photoaging damage. When the ultraviolet radiation intensity is greater than 3.7 mJ / cm per day 2 When it occurs, it can destroy the redox homeostasis of...

Claims

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Application Information

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IPC IPC(8): C07K7/08C07K1/20A61K38/10A61K8/64A61P39/06A61Q19/08A61Q17/04A23L33/18
CPCC07K7/08A61K8/64A61P39/06A61Q19/08A61Q17/04A23L33/18A61K38/00A23V2002/00A23V2200/318
Inventor 杨新旺王滢尹赛格陶剑赵建孙慧玲谢纯张心平李依林
Owner KUNMING MEDICAL UNIVERSITY
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