Anti-oxidative injury skin-protection active polypeptide AOP-P1 and preparation method and application thereof

A technology of AOP-P1 and skin protection, applied in the field of biomedicine, can solve the problems of low activity, difficulty in large-scale synthesis, toxicity, carcinogenicity, and poor stability, and achieve high antioxidant activity, great medical application prospects, and high stability Effect

Inactive Publication Date: 2019-12-20
KUNMING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the most widely used antioxidants have different defects, such as low activity, poor stability, difficulty in large-scale synthesis, and even potential toxicity and carcinogenicity.

Method used

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  • Anti-oxidative injury skin-protection active polypeptide AOP-P1 and preparation method and application thereof
  • Anti-oxidative injury skin-protection active polypeptide AOP-P1 and preparation method and application thereof
  • Anti-oxidative injury skin-protection active polypeptide AOP-P1 and preparation method and application thereof

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preparation example Construction

[0021] The preparation method of the anti-oxidative damage skin protection active polypeptide AOP-P1 of the present invention comprises the following steps:

[0022] A. Take the skin secretion of the Yunnan stink frog by electrical stimulation, dissolve it in Tris-HCl, freeze-dry it in vacuum to obtain material a, and store it at -80°C for later use;

[0023] B. Dissolving material a in water to obtain material b, material b is eluted and fractionated to obtain sample c;

[0024] C. The sample c is subjected to high-performance liquid chromatography reversed-phase chromatography to obtain the target anti-oxidative damage skin protection active polypeptide AOP-P1.

[0025] The water described in the B step is deionized water.

[0026] The elution described in step B was eluted on a Sephadex G-75 gel filtration column with TriS-HCl (pH 7.8) mixed with NaCl as the elution buffer.

[0027] The fractionation described in step B is to use an automatic split collector to collect fr...

Embodiment 1

[0032] 1. Sample Collection and Animal Care

[0033] Adult yunnanensis frogs were collected from Lijiang City, Yunnan Province and transported safely to the laboratory. The stink frogs were housed en masse in containers with mealworms ready for consumption by the stink frogs. After acclimating to the environment for a week, the frog skin was stimulated with an electric massager with 6 volts alternating current. Then wash the skin secretions of the stink frog with Tris-HCl buffer (pH7.8). The obtained secretion was centrifuged at 4000 g for 15 min (4 °C), and the supernatant was collected and lyophilized. All samples were stored at -80°C until further analysis.

[0034] 2. Purification procedure

[0035] Freeze-dried skin secretion samples were dissolved in deionized water and applied to a Sephadex G-75 gel filtration column, and eluted with TriS-HCl (pH 7.8) mixed with NaCl as the elution buffer. Fractions were collected every 10 min using an automated fraction collector ...

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Abstract

The invention discloses an anti-oxidative injury skin-protection active polypeptide AOP-P1 and a preparation method and application thereof. The amino acid sequence of the anti-oxidative injury skin-protection active polypeptide AOP-P1 is FLPGLECVW. The method extracts the anti-oxidation active polypeptide from odorrana andersonii skin secretion. The polypeptide has the characteristics of naturalsource, high activity and the like. The purified anti-oxidative injury skin-protection active polypeptide AOP-P1 shows high stability and oxidation resistance, and can effectively protect skin from light damage caused by ultraviolet irradiation. The invention shows that the anti-oxidative injury skin-protection active polypeptide AOP-P1 has larger medical application prospect. The anti-oxidative injury skin-protection active polypeptide AOP-P1 disclosed by the invention has the beneficial characteristic of being used as an anti-ultraviolet drug candidate.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an anti-oxidative damage skin protection active polypeptide AOP-P1 and its preparation method and application. Background technique [0002] Over the past few years, with the increase in environmental pollution and the depletion of the Earth's ozone layer, the levels of ultraviolet (UV) exposure, especially UVA and UVB, have severely increased. UV radiation (280-320 nm) is the most harmful UV to human skin and it can cause oxidative stress in the body. When the ultraviolet radiation intensity is greater than 3.7mJ / cm2 / day, it will destroy the redox balance of the skin. Antioxidants in the body will decrease and the accumulation of peroxidation products will increase. These oxidative damages allow skin photodamage to occur and eventually lead to the development of skin cancer. The most widely used antioxidants have different defects, such as low activity, poor s...

Claims

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Application Information

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IPC IPC(8): C07K7/08C07K1/20A61K38/10A61K8/64A61P39/06A61Q19/08A61Q17/04A23L33/18
CPCC07K7/08A61K8/64A61P39/06A61Q19/08A61Q17/04A23L33/18A61K38/00A23V2002/00
Inventor 杨新旺王滢孙俊尹赛格杨莹陶剑刘乃心杨美凤傅阳谢纯李依林张心平
Owner KUNMING MEDICAL UNIVERSITY
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