Antimicrobial lotion containing wood frog antimicrobial peptide and preparation method thereof

An antimicrobial peptide and lotion technology, which is applied in antifungal agents, medical preparations containing active ingredients, antibacterial drugs, etc., can solve the problems of not obvious bactericidal effect and strong irritation of antibacterial agents, and achieve remarkable antibacterial and bactericidal effects , the skin is soft and smooth, and the antibacterial activity time is prolonged

Active Publication Date: 2009-08-05
TONGHUA KANGYUAN BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide an antibacterial lotion containing Rana antibacterial peptide, which has the functions of sterilization, antivir...

Method used

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  • Antimicrobial lotion containing wood frog antimicrobial peptide and preparation method thereof
  • Antimicrobial lotion containing wood frog antimicrobial peptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Soak 120 g of dried frog skin with 1300 ml of 4% acetic acid solution at a ratio of 1:10-15 (m / v) for 24 hours (at room temperature).

[0033] (2) Centrifuge the obtained extract at 8000 r.p.m for 15 min, discard the precipitate and keep the supernatant.

[0034] (3) Boil the supernatant for 5 minutes, cool down, centrifuge at 8000 r.p.m for 15 minutes, and pass the supernatant through a 30KDa membrane.

[0035] (4) Freeze-dry the over 30KDa film solution, dissolve 0.0005wt% freeze-dried powder in 1800mL of 0.25M pH 5.5 phosphate buffer, add 90mL of 5wt% mannitol to make light yellow or brown yellow transparent liquid to obtain antibacterial lotion.

Embodiment 2

[0037] (1) Soak 200 g of dried frog skin with 2500 ml of 4% acetic acid solution at a ratio of 1:10-15 (m / v) for 24 hours (at room temperature).

[0038] (2) Centrifuge the obtained extract at 8000 r.p.m for 15 min, discard the precipitate and keep the supernatant.

[0039] (3) Boil the supernatant for 5 minutes, cool down, centrifuge at 8000 r.p.m for 15 minutes, and pass the supernatant through a 30KDa membrane.

[0040] (4) Freeze-dry the over 30KDa film solution, dissolve 0.005wt% freeze-dried powder in 0.25M pH6.0 phosphate buffer 4200mL, add 10wt% mannitol 600mL to make light yellow or brown yellow transparent liquid Antibacterial lotion.

Embodiment 3

[0042] (1) Soak 320 g of dried frog skin with 4500 ml of 4% acetic acid solution at a ratio of 1:10-15 (m / v) for 24 hours (at room temperature).

[0043] (2) Centrifuge the obtained extract at 8000 r.p.m for 15 min, discard the precipitate and keep the supernatant.

[0044] (3) Boil the supernatant for 5 minutes, cool down, centrifuge at 8000 r.p.m for 15 minutes, and pass the supernatant through a 30KDa membrane.

[0045] (4) Freeze-dry the over 30KDa film solution, dissolve 0.05wt% freeze-dried powder in 0.25M pH6.5 phosphate buffer 7500mL, add 20wt% mannitol 1500mL to make light yellow or brown yellow transparent liquid Antibacterial lotion.

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Abstract

The invention relates to an antibacterial lotion containing rana antibacterial peptides and a preparation process thereof. The rana antibacterial peptides are dissolved in a phosphate buffer with the pH of between 5 and 7 and are added with a mannitol protecting agent to prepare a light yellow transparent liquid, namely the antibacterial lotion. The lotion has the functions of sterilization, disinfection, itching relief, astringency and hemostasis, fever removal and insecticide, and the like, is non-toxic and harmless to a human body, does not irritate skin and mucous membrane, can be widely applied to sanitation and cleanness of human skin and mucous membrane, antibacterial and sterilization and other fields with sterilizing effect reaching 100 percent. Furthermore, due to the addition of the mannitol protecting agent, the duration of effective antibiotic activity of the antibacterial lotion is greatly prolonged.

Description

Technical field: [0001] The invention relates to a product made of antibacterial peptide obtained from amphibian Rana, in particular to an antibacterial lotion containing Rana antibacterial peptide and a preparation method thereof, belonging to the technical field of preparation of washing products. Background technique; [0002] Most of the antibacterial lotion products sold on the market are chemically synthesized products, because they contain chemical components, which can easily cause different degrees of irritation and other toxic and side effects on the human body. Such as chlorhexidine acetate and benzyl ammonium bromide as the main components of antibacterial lotion, its antibacterial effect can reach 90% in more than 10 minutes, has no obvious bactericidal effect, and has a very strong irritating sour taste, which is harmful to the skin and mucous membranes. irritating. In contrast, the commercially available traditional Chinese medicine lotion is 100% suspension,...

Claims

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Application Information

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IPC IPC(8): A61K8/64A61K38/17A61K9/08A61Q19/10A61P31/04A61P31/10A61P31/16A61P31/18A61P31/20
Inventor 滕利荣孟庆繁逯家辉安金双李青山周杰田晓乐高朝辉王贞佐
Owner TONGHUA KANGYUAN BIOLOGICAL TECH
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