The invention belongs to the technical field of traditional Chinese medicine, and provides a composition for skin sterilization, deodorization, itching relief and parasite expelling and a preparationmethod thereof, aiming to solve the technical problems of proneness to reoccurrence of animal skin diseases, irritability, intoxication and slow growth symptoms in the prior art. The composition comprises, by weight, 2-5 parts of radix stemonae, 2-4 parts of pericarpium zanthoxyli, 1-3 parts of cloves, 1-3 parts of ligusticum wallichii, 3-5 parts of radix sophorae flavescentis, 1-4 parts of cortexdictamni, 1-3 parts of myrrh, 3-5 parts of rhizoma atractylodis, 1-3 parts of folium isatidis, 1-3 parts of motherwort, 2-4 parts of wild chrysanthemums, 2-3 parts of honeysuckle stems, 1-3 parts ofcicada sloughs, 1-3 parts of belvedere fruits, 1-2.5 parts of red peony roots, 3-5 parts of rhizoma smilacis glabrae, 1-3.2 parts of cortex erythrinae, 1.5-3.5 parts of fructus cnidii, 1-3 parts of borneol, 1.5-3 parts of angelica sinensis, 1-3 parts of cortex phellodendri, 1-3 parts of mint and 1-3 parts of sulphur. The composition has the advantages of sterilization, itching relief, deodorization, parasite expelling, hair beautifying, heat clearing, detoxication, pathogenic microorganism resistance, anti-inflammation and pain relief.