Multifunctional natural compound and application thereof

Abstract

The invention discloses a multifunctional natural compound and application thereof, and belongs to the technical field of natural compounds. The multifunctional natural compound disclosed by the invention is prepared from sophorolipid, natural essential oil and polyhydric alcohols. According to the invention, safe and nontoxic sophorolipid, natural essential oil and polyol are compounded, so that the broad-spectrum antibacterial effect is remarkable, and particularly, the antibacterial effect on staphylococcus aureus and propionibacterium acnes is improved by 4-8 times; and the prepared natural compound can stably coexist with various daily chemical components, does not influence the texture and efficacy of the product, ensures the stable quality of the product, has good effects (including corrosion prevention, bacteriostasis, acne removal, dandruff removal, itching relieving and the like) in the application of face cream, essence, face cleaning mousse and shampoo, and can reduce the potential harm of chemical source additives to the environment and human health.

Description

Technical Field

[0001] The present invention belongs to the technical field of natural compound, and more specifically, relates to a multifunctional natural compound and its application. Background Art

[0002] With the increasing demand of consumers for the integration of health, environmental protection and efficacy, the daily chemical industry is accelerating its transformation towards naturalization, functionality and greenness. Among cosmetics, preservatives are one of the essential additives, which are used to inhibit the rapid growth and reproduction of microorganisms after the product is opened, so as to ensure the stable efficacy of cosmetics and improve their use value.

[0003] However, among many products, more chemical synthetic preservatives (such as benzoic acid and sorbic acid) are added. Chemical synthetic preservatives are slightly more irritating, have a high skin sensitization rate, and will cause harm to the human body after long-term use. Therefore, natural, non-toxic, non-irritating and non-skin allergic safe and highly efficient antibacterial active substances have become a new development direction for the anti-corrosion and antibacterial of daily chemical products, and have good development prospects.

[0004] Sophorolipid is a kind of glycolipid surfactant obtained by microbial fermentation and transformation, which has good emulsifying property, surface / interface activity, biodegradability, etc. At the same time, it has physiological activities such as antibacterial activity, induction of apoptosis and mutation of tumor cells, and has potential application value in the fields of food, cosmetics, environmental protection, medicine, materials, etc. Although some natural plant extracts have antibacterial properties, the antibacterial spectrum of single components is narrow and the bactericidal rate is low. They need to be compounded with chemical preservatives and are difficult to completely replace synthetic preservatives. However, there is currently no report on the application of the combination of sophorolipid, natural essential oil and 1,2-pentanediol as a preservative. Summary of the Invention

[0005] Aiming at the above problems existing in the prior art, the technical problems to be solved by the present invention are to provide a multifunctional natural compound with good antibacterial effect and outstanding anti-corrosion effect, which can be safely used in daily necessities. Another technical problem to be solved by the present invention is to provide the application of the multifunctional natural compound in preservatives, antibacterial agents, additives for daily chemical products, pet washing and care products, skin care cosmetics, skin cleansing cosmetics, and the preparation of scalp care products.

[0006] In order to solve the above technical problems, the technical solutions adopted by the present invention are as follows:

[0007] A multifunctional natural compound is compounded by sophorolipid, natural essential oil and polyol, and the mass ratio of sophorolipid, natural essential oil and polyol is 1-6:0.5-2:3-7.

[0008] Preferably, the mass ratio of sophorolipid, natural essential oil and polyol is 2-4:0.5-1:4-6. More preferably, the mass ratio of sophorolipid, natural essential oil and polyol is 4:1:5.

[0009] Preferably, the natural essential oil is selected from any one of cinnamon essential oil, lemon essential oil, clove essential oil, peppermint essential oil, eucalyptus oil.

[0010] Preferably, the natural essential oil is cinnamon essential oil.

[0011] Preferably, the sophorolipid is selected from any one of acidic sophorolipid and lactone-type sophorolipid.

[0012] Preferably, the sophorolipid is lactone-type sophorolipid S L-active.

[0013] Preferably, the polyol has 5-6 carbon atoms.

[0014] Preferably, the polyol is selected from any one of 1,2-pentanediol, hexylene glycol, dipropylene glycol. More preferably, the polyol is 1,2-pentanediol.

[0015] Application of the multifunctional natural compound in preparing preservatives and / or bacteriostatic agents.

[0016] Application of the multifunctional natural compound in preparing additives for daily chemical products, additives for pet washing and care products, additives for skin care cosmetics, additives for skin cleansing cosmetics, additives for scalp care products.

[0017] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0018] (1) The present invention uses the compound of sophorolipid, natural essential oil and polyol, which has the characteristics of safety, high efficiency, non-toxic and no side effects, and has a significant broad-spectrum bacteriostatic effect. Especially, the bacteriostatic effect on Staphylococcus aureus and Propionibacterium acnes is increased by 4-8 times;

[0019] (2) The compound of sophorolipid, natural essential oil and polyol in the present invention has a synergistic effect, enhances the anti-corrosion effect, prolongs the product shelf life, and does not affect the product texture and sensory experience, and is more suitable for sensitive skin; in addition, the compound has good compatibility, can coexist stably with a variety of daily chemical ingredients, does not affect the product texture and efficacy, ensures the product quality stability, and has good effects in the application of cream, essence, facial cleansing mousse, shampoo, including anti-corrosion, bacteriostasis, acne removal, dandruff removal and itching relief, etc.;

[0020] (3) All raw materials used in the present invention are safe and non-toxic, achieving the pure naturalness and non-toxic side effects of the natural compound, conforming to the concept of green environmental protection, reducing the potential harm of chemically sourced additives to the environment and human health, and meeting consumers' demands for natural and healthy daily chemical products. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a physical diagram of Product 3 obtained in Example 1;

[0022] Figure 2 It is a diagram of the antibacterial experiment results of Product 3 obtained in Example 1 in different cosmetics; among them, a is the result diagram in essence, b is the result diagram in facial cleansing foam, and c is the result diagram in shampoo;

[0023] Figure 3 It is a diagram of the minimum inhibitory concentration test results of Product 3 obtained in Example 1 against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Aspergillus niger, and Corynebacterium xerosis;

[0024] Figure 4 It is a diagram of the minimum inhibitory concentration test results of Product 3 obtained in Example 1 against Propionibacterium acnes and Malassezia furfur; among them, a is the antibacterial test result diagram against Propionibacterium acnes, and b is the antibacterial test result diagram against Malassezia furfur;

[0025] Figure 5 It is a diagram of the stability results of Product 3 obtained in Example 1; among them, a is the result diagram before the inspection, and b is the result diagram after one month of inspection;

[0026] Figure 6 It is a diagram of the stability results of Product 3 obtained in Example 1 in essence; among them, a is the result diagram before the inspection, and b is the result diagram after one month of inspection;

[0027] Figure 7 It is a diagram of the stability results of Product 3 obtained in Example 1 in facial cleansing foam; among them, a is the result diagram before the inspection, and b is the result diagram after one month of inspection;

[0028] Figure 8 It is a diagram of the stability results of Product 3 obtained in Example 1 in shampoo; among them, a is the result diagram before the inspection, and b is the result diagram after one month of inspection;

[0029] Figure 9 It is a diagram of the stability results of Product 3 obtained in Example 1 in cream; among them, a is the result diagram before the inspection, and b is the result diagram after one month of inspection;

[0030] Figure 10 It is a diagram of the minimum inhibitory concentration test results of cinnamon essential oil;

[0031] Figure 11 It is the graph of the minimum inhibitory concentration test results of lemon essential oil;

[0032] Figure 12 It is the graph of the minimum inhibitory concentration test results of clove essential oil;

[0033] Figure 13 It is the graph of the minimum inhibitory concentration test results of peppermint essential oil;

[0034] Figure 14 It is the graph of the minimum inhibitory concentration test results of eucalyptus oil. Specific embodiments

[0035] To make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described below in conjunction with specific embodiments. In the following embodiments, unless otherwise specified, the technical means used are all conventional means well-known to those skilled in the art. For those conditions not specified in the embodiments, they are carried out according to conventional conditions or the conditions recommended by the manufacturer. For the reagents or instruments whose manufacturers are not specified, they are all conventional products that can be obtained through commercial purchase.

[0036] The test process of the test method used in the following embodiments is as follows:

[0037] 1. Minimum inhibitory concentration test, and the test process is as follows:

[0038] 1) Test bacteria: Staphylococcus aureus (ATCC6538), Escherichia coli (ATCC8739), Pseudomonas aeruginosa (ATCC 9027), Candida albicans (ATCC10231), Aspergillus niger (ATCC16404), Corynebacterium xerosis (ATCC 373), Propionibacterium acnes (ATCC 6919), Malassezia furfur (ATCC 44344). Prepare the bacterial suspension of each strain into 1*10 7 cfu / mL;

[0039] 2) Preparation of sample diluent: Accurately weigh 1.0 g of the sample, add sterile deionized water (or solubilizer) to a total weight of 10.0 g to prepare the sample stock solution. Take 5 mL of the sample stock solution and add it to a test tube containing 5 mL of sterile deionized water for mixing. Then take 5 mL of the diluent from this tube and add it to the next test tube containing 5 mL of sterile deionized water for mixing, and perform serial two-fold dilutions in sequence to obtain a series of dilutions of the sample gradient;

[0040] 3) Preparation of sample agar: Weigh MHA agar medium (the medium for Propionibacterium acnes is GAM, and the medium for Malassezia furfur is malt extract agar medium), heat and dissolve it, dispense it into bottles at 18 mL / bottle, and then sterilize it in a high-pressure steam sterilizer at 121 °C for 15 min (the GAM medium is sterilized at 115 °C). When the temperature of the agar medium drops to about 50 °C, add 2 mL of sample dilution solution respectively, mix well, pour the plates, and wait for them to solidify. Agar plates with different sample concentrations are obtained. In addition, use sterile deionized water (or solubilizer) as a control;

[0041] 4) Bacterial inoculation and culture: Mark the inoculation sites of each bacterium on the back of the MHA agar plate medium (GAM medium, malt extract agar medium) and spot 2 μL of the bacterial suspension at the corresponding marked positions. Let it stand for a while, and culture the MHA agar plate in an incubator at 36 °C for 18 h to 48 h (the GAM agar plate is anaerobically cultured in an incubator at 36 °C for 24 h to 48 h, and the malt extract agar plate is cultured in an incubator at 28 °C for 48 h to 72 h);

[0042] 5) After culturing, observe the growth of bacteria in the sample plates. The minimum inhibitory concentration of the target bacterium is the lowest sample concentration at which no bacteria grow.

[0043] The sophorolipid used in the following examples is a commercially available product from Nanjing Specbio Biotechnology Co., Ltd. SL-Active. Cinnamon essential oil, clove essential oil, lemon essential oil, peppermint essential oil, and eucalyptus oil are all purchased from Jiangxi Global.

[0044] Example 1

[0045] The process of preparing the multifunctional natural compound is as follows: Weigh the raw materials according to the formula in Table 1 below, and stir and mix the raw materials at room temperature to obtain the multifunctional natural compound.

[0046] Table 1 Formulas of Products 1 - 6

[0047]

[0048]

[0049] Control Example

[0050] When preparing the compound, weigh the raw materials according to the formula in Table 2 below, and stir and mix the raw materials at room temperature to obtain the compound.

[0051] Table 2 Formulas of Control Examples 1 - 6

[0052]

[0053] Example 2

[0054] The minimum inhibitory concentration tests were carried out on the products prepared from Products 1-6, Comparative Examples 1-6, and a commercially available product bacteriostatic agent. The results are shown in Table 3 and Figures 3 - 4 as follows. The commercially available products used were: The oil-soluble moutan bark antibacterial agent was purchased from MST, Korea, with a recommended dosage of 0.1% - 2%, and it was a light yellow transparent liquid; the moutan bark bacteriostatic agent was purchased from MST, Korea, with a recommended dosage of 0.5% - 2.0%, and it was a light yellow transparent liquid; The formula of VLC was cinnamon bark extract, lemon fruit extract, soybean seed extract, and butylene glycol, and it was a transparent liquid; The formula of VMC was moutan root bark extract, hinoki leaf extract, lemon fruit extract, soybean seed extract, ethylhexylglycerin butylene glycol, and it was a transparent liquid; The formula of PE91 was phenoxyethanol and ethylhexylglycerin, and it was a colorless transparent liquid; CHP was a colorless transparent liquid.

[0055] Table 3 Minimum inhibitory concentration results of the bacteriostatic agents of the products prepared from Products 1-6, Comparative Examples 1-6, and commercially available products

[0056]

[0057]

[0058] As can be seen from Table 3, the addition of SL-Active improved the bacteriostatic effect of the compound, especially the bacteriostatic effect on Staphylococcus aureus and Propionibacterium acnes was increased by 4 - 8 times. Comparing Products 1-5, it was found that when the sophorolipid content was increased to 40% - 60%, the minimum inhibitory concentration of the compound against Pseudomonas aeruginosa decreased from 1.0000% to 0.5000%, improving the overall bacteriostatic effect. Product 3 had a more prominent bacteriostatic effect and a wider bacteriostatic spectrum compared to Comparative Examples 2-6.

[0059] Example 3

[0060] Product 3 was placed at -18°C, 4°C, RT, under light, and 45°C for 1 month respectively, and whether there were color changes, turbidity, precipitation, etc. was observed. The results are as Figure 5 follows.

[0061] As Figure 5 can be seen, after being placed for one month, the prepared natural compound showed varying degrees of color change. Therefore, the prepared natural compound needs to be stored at room temperature in a brown bottle.

[0062] Add 1.0% of Product 3 to the essence, facial cleansing mousse, shampoo, and cream in Example 4 respectively, and place them at -18°C, 4°C, under light, RT, and 45°C for 1 month, and observe whether there are color changes, turbidity, precipitation, etc. The results are as Figures 6 - 9 shown.

[0063] As Figures 6 - 9 can be seen, after being placed for one month, the essence, facial cleansing mousse, and shampoo placed at 45°C changed color slightly. There were no color changes, turbidity, precipitation, etc. in the formulated products under other storage conditions.

[0064] Example 4

[0065] Conduct performance tests on Product 3 in cosmetics. The formulations of the essence, facial cleansing mousse, shampoo, and cream used are shown in Table 4-7 below.

[0066] Table 4 Essence Formulation

[0067]

[0068]

[0069] Table 5 Shampoo Formulation

[0070]

[0071] Table 6 Facial Cleansing Mousse Formulation

[0072]

[0073]

[0074] Table 7 Cream Formulation

[0075]

[0076]

[0077] 1. Test the antibacterial properties of different addition amounts of Product 3 against Malassezia furfur and Propionibacterium acnes in cosmetic applications. The test process is as follows:

[0078] (1) Test bacteria: Propionibacterium acnes (ATCC 6919), Malassezia furfur (ATCC 44344), and prepare the bacterial strains into bacterial suspensions;

[0079] (2) Preparation of culture medium petri dishes: Cool the sterilized GAM agar culture and malt extract agar medium to 45 - 50°C, pour 15 - 20 mL of the sterilized and cooled medium into each sterile petri dish, and wait for it to cool and solidify; Preparation of inoculated petri dishes: Take 100 μL of the bacterial suspension of the target bacteria and evenly coat it on the prepared culture medium petri dishes;

[0080] (3) Sample addition and detection: Take the sterilized and dried Oxford cups and stand them on the Petri dishes with bacteria. Add 200 μL of the sample solution to each well, and set the blank formula without preservatives as the control. Incubate at 36 °C for 24 - 48 h (Malassezia furfur is incubated at 28 °C for 48 - 72 h) and observe the antibacterial effect.

[0081] (4) Determine the sensitivity according to the diameter of the antibacterial zone. The judgment criteria are as follows: antibacterial zone diameter = 0 mm, insensitive; 0 < antibacterial zone diameter < 10 mm, low sensitivity; 10 mm ≤ antibacterial zone diameter ≤ 15 mm, medium sensitivity; 15 mm < antibacterial zone diameter < 20 mm, high sensitivity; 20 mm ≤ antibacterial zone diameter, extremely sensitive. The results are shown in Table 8 and Figure 2 as follows.

[0082] Table 8 Antibacterial test results of Product 3 with different addition amounts in cosmetics

[0083]

[0084] As can be seen from Table 8, when Product 3 is applied in facial skin care, it can strengthen the inhibition of Propionibacterium acnes growth and reduce the formation of acne and comedones. When Product 3 is applied in hair washing, it can strengthen the inhibition of Malassezia furfur growth and reduce the generation of dandruff.

[0085] 2. Antiseptic performance test of Product 3 in cosmetics. The test process is as follows:

[0086] (1) Preparation of bacterial suspension: Take freshly prepared Staphylococcus aureus (ATCC6538), Escherichia coli (ATCC8739), Pseudomonas aeruginosa (ATCC 9027), Candida albicans (ATCC 10231), and Aspergillus niger (ATCC 16404) spore suspensions. Use an enzyme-linked immunosorbent assay (ELISA) reader to adjust the bacterial concentration to 1*10 8 ~5*10 8 cfu / mL, and adjust the fungal concentration to 1*10 7 ~5*10 7 cfu / mL. Mix Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa in a volume ratio of 1:1:1 to prepare a mixed bacterial suspension. Mix Candida albicans and Aspergillus niger spore suspensions in a volume ratio of 1:1 to prepare a fungal suspension;

[0087] (2) Sample sub-packaging and bacteria addition: Sub-package the sample into 2 bottles of 50 g samples. Add 0.5 mL of the mixed bacteria to one bottle and 0.5 mL of the fungal suspension to the other bottle.

[0088] (3) Test the microbial content in the samples at 0 day, 7 days, 14 days, and 28 days respectively. The judgment criteria are as follows: 1) Bacteria: The antibacterial rate in the anti-corrosion challenge at the 7th day ≥ 99.9% (the logarithm of the reduction in the number of colonies ≥ 3.0), and in each subsequent challenge, the number of colonies does not increase; 2) Yeasts and molds: The antibacterial rate in the anti-corrosion challenge at the 7th day ≥ 90.0% (the logarithm of the reduction in the number of colonies ≥ 1.0), and in each subsequent challenge, the number of colonies does not increase. The results are shown in Table 9.

[0089] Table 9 Anti-corrosion effects of Product 3 in different cosmetics

[0090]

[0091]

[0092] As can be seen from Table 9, Product 3 can pass the anti-corrosion challenge test in the above-mentioned essence, cream, facial cleansing mousse, and shampoo, showing excellent and stable anti-corrosion performance.

[0093] Example 5

[0094] Perform the minimum inhibitory concentration test on commercially available glycolipids and sophorolipids used in this application. The results are shown in Table 10. The specific information of the commercially available products used is as follows:

[0095] Market sample 1 is BIOTOLIFE, batch number NM37, which is an amber viscous liquid, pH (5%) is 4.2, Color (5%, Hazen) is 248.7, and the solid content is 51.4%.

[0096] Market sample 2 is Sophorolipids purchased from HoliSurf LF Biosurfactant, which is an amber liquid, pH is 5 - 7, Color (Gardner) < 8.5, and the solid content is 55% - 65%.

[0097] Market sample 3 is LA-A, batch number S023271923, which is an amber viscous liquid, pH (5%) is 4.8, Color (5%, Hazen) is 91.98, and the solid content is 49.1%.

[0098] The SL-Active, model number 19001104, which is a yellow liquid, pH (5%) is 4.7, Color (Gardner) is 8.6, and the solid content is 66%.

[0099] Table 10 Results of the minimum inhibitory concentration of commercially available glycolipids and sophorolipids used in the present invention

[0100]

[0101] As can be seen from Table 10, The minimum inhibitory concentrations of SL-Active against Staphylococcus aureus, Corynebacterium xerosis, and Propionibacterium acnes are 0.0625%, 0.00313%, and 0.0078% respectively, which are better than those of market samples.

[0102] Example 6

[0103] The minimum inhibitory concentrations of cinnamon essential oil, lemon essential oil, clove essential oil, peppermint essential oil, and eucalyptus oil used in this application were tested, and the results are shown in Table 11 and Figures 10 - 14 as follows.

[0104] Table 11 Results of the minimum inhibitory concentrations of different essential oils

[0105]

[0106]

[0107] As can be seen from Table 11, cinnamon essential oil has a relatively wide antibacterial spectrum and the best antibacterial effect.

[0108] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. A multifunctional natural compound, characterized in that, It is composed of sophorolipid, natural essential oil and polyol, and the mass ratio of sophorolipid, natural essential oil and polyol is 1-6:0.5-2:3-7.

2. The multifunctional natural complex according to claim 1, wherein The mass ratio of the sophorolipid, natural essential oil and polyol is 2-4:0.5-1:4-6.

3. The multifunctional natural compound according to claim 1, characterized in that, The natural essential oil is selected from any one of cinnamon essential oil, lemon essential oil, clove essential oil, peppermint essential oil, eucalyptus oil.

4. The multifunctional natural complex according to claim 3, characterized in that, The natural essential oil is cinnamon essential oil.

5. The multifunctional natural complex according to claim 1, wherein, The sophorolipid is selected from any one of acid-type sophorolipid and lactone-type sophorolipid.

6. The multifunctional natural compound according to claim 5, characterized in that, The sophorolipid is lactone-type sophorolipid S L-active.

7. The multifunctional natural compound according to claim 1, characterized in that, The polyol has 5-6 carbon atoms.

8. The multifunctional natural compound according to claim 7, characterized in that, The polyol is selected from any one of 1,2-pentanediol, hexanediol, dipropylene glycol.

9. Use of the multifunctional natural complex according to any one of claims 1-8 in the preparation of a preservative and / or bacteriostatic agent.

10. Use of the multifunctional natural complex according to any one of claims 1-8 in the preparation of additives for daily chemical products, additives for pet washing and care products, additives for skin care cosmetics, additives for skin cleansing cosmetics, additives for scalp care products.

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