Rana grahami boulenger antibiotic peptide, preparation method and application thereof

An antimicrobial peptide and stinky frog technology, applied in the fields of biochemistry and biomedicine, can solve the problems of failure of anti-infection treatment, increase of medical expenses, increase of morbidity and mortality

Inactive Publication Date: 2007-10-24
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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Problems solved by technology

Bacteria develop resistance to antibiotics, leading to the failure of anti-infection treatment, which will inevitably lead to an increase in morbidity and mortality and a substantial increase in medical expenses. Therefore, the resistance of pathogens to antibiotics is the most serious problem facing global public health in the 21st century. one of the threats

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  • Rana grahami boulenger antibiotic peptide, preparation method and application thereof
  • Rana grahami boulenger antibiotic peptide, preparation method and application thereof
  • Rana grahami boulenger antibiotic peptide, preparation method and application thereof

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Embodiment Construction

[0031] The specific embodiments will help those of ordinary skill in the art to further understand the present invention, but do not limit the scope of the present invention in any form. Experimental methods that do not indicate specific conditions in the implementation methods can usually be carried out according to conventional conditions, such as "Molecular Cloning Experiment Guide" (J. Sambrook, etc.) and "Refined Molecular Biology Experiment Guide" (F.M. Osper, etc.) conditions described in , or in accordance with the conditions recommended by the manufacturer.

[0032] Preparation of Odorgrin A, an antibacterial peptide from stinkfrog:

[0033] Acquisition of the crude extract: Wash the frog with double-distilled water, put it in a beaker, add double-distilled water, and stimulate it with 25V for 1 minute, then re-stimulate it after 2 minutes, and stimulate it 5 times in total. Secretion, the secretion was centrifuged for 30 minutes, and the supernatant was freeze-dried...

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Abstract

The invention discloses a non-finger disc odor-frog antibiotic peptide and making method in the biochemical and biological medical domain, which comprises the following steps: stimulating the active non-finger disc odor frog through electricity; collecting secretion; centrifuging; removing sediment; freezing; drying; proceeding ion exchange; gelling; filtering; proceeding reverse-phase chromatographic separation to purify; obtaining the peptide named as OdorgrinA with 33 amino acids, theoretical molecular weight at 3365. 81D, real molecular weight at 3365. 24D, theoretical isoelectric point (pI) at 9. 31 and whole sequent structure at NH2-GLLDTFKNLALNAAKSAGVSVLNSLSCKLSKTC-COOH. The invention possesses broad spectrum activity, which can used to produce antibiotic drug and antibiotic agent.

Description

technical field [0001] The invention belongs to the fields of biochemistry and biomedicine, and relates to OdorgrinA, an antimicrobial peptide secreted from the skin of a fingerless stink frog, and a preparation method and application thereof. Background technique [0002] The discovery of antibiotics is the most brilliant achievement of modern medicine in the 20th century. Thanks to the clinical application of antibiotics, many infectious diseases that were once considered fatal have been cured, thereby significantly increasing human life expectancy. However, due to the misuse and irrational use of antibiotics in the past 30 years, the widespread spread of drug-resistant strains has made anti-infection treatment a thorny issue. For example, acute respiratory infections have become difficult to control due to the emergence of Streptococcus pneumoniae resistance to penicillin, macrolide antibiotics, and fluoroquinolone antibiotics. Vancomycin, whic...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/14A61K38/17A61P31/04
CPCY02A50/30
Inventor 江建平刘炯宇谢锋
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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