Production process for amino acids of the aspartate family using microorganisms

A technology of aspartic acid and microorganisms, applied in the application field of L-lysine and its precursors, L-lysine and its precursors, can solve the problems of low production performance, paramutation, weakened growth, etc.

Inactive Publication Date: 2012-10-24
PAIK KWANG IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This results in efficient producers, but also leads to the accumulation of side mutations that cause reduced growth, poor stress tolerance, or increased nutrient requirements
Therefore, for C. glutamicum, the currently obtained production performance is significantly lower than the predicted theoretical capacity

Method used

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  • Production process for amino acids of the aspartate family using microorganisms
  • Production process for amino acids of the aspartate family using microorganisms
  • Production process for amino acids of the aspartate family using microorganisms

Examples

Experimental program
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Embodiment

[0225] In the following examples, recombinant DNA technology and authoritative techniques of molecular biology are used, as described in various publications, such as Sambrook et al. (2001), Molecular Cloning: A Laboratory Manual, 3rd Edition, Cold Spring Harbor Experiment Chamber Press, or Ausubel et al. (2007), Current Protocols in Molecular Biology, Current Protocols in Protein Science, 2002 edition, Wiley Interscience. All cells, reagents, devices and kits were used according to the manufacturer's instructions unless otherwise stated.

[0226] Materials and methods

[0227] strain

[0228] All C. glutamicum strains were derived by stable genetic engineering from wild-type ATCC 13032 (American Type Culture Collection, Manassas, VA, USA). Escherichia coli DH5α and NM522 were purchased from Invitrogen (Karlsruhe, Germany) and used for vector amplification and DNA methylation, respectively. All strains are listed in Table 2.

[0229] Table 2: Strains used in the present ...

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Abstract

The present invention is directed to a method utilizing a recombinant microorganism for the production of aspartate derived amino acids and precursors therof, in particular for the production of L-lysine. Furthermore, the present invention relates to a recombinant microorganism having improved aspartate-derived amino acid synthesis activity in comparison to the initial microorganism and the use of such microorganisms in producing said amino acids and precursors and derivatives, in particular in the synthesis of L-lysine.

Description

technical field [0001] The present invention relates to a method for producing aspartic acid group amino acid, especially L-lysine and its precursor by using recombinant microorganism. In addition, the present invention also relates to a recombinant microorganism, and the use of this microorganism in the production of aspartic acid group amino acids, especially L-lysine and its precursors, the recombinant microorganism has a modified Synthetic pathway of aspartate amino acids. Background technique [0002] Amino acids and their derivatives are important precursors in the pharmaceutical industry and they are added as supplements to a wide variety of foods and feeds. Several amino acids, such as glutamic acid, lysine and threonine are produced using their natural biosynthetic pathways. In natural amino acid biosynthesis, the amino acid aspartate serves as a precursor for the production of other amino acids such as lysine, threonine, isoleucine and methionine. [0003] With ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/63C12P13/08C12P13/20C12N15/77C12R1/15
CPCC12P13/08C12N9/1217C12N9/16C12P13/20C12N15/52C12N9/0006C12N1/20C12N15/79
Inventor C·维特曼J·贝克尔
Owner PAIK KWANG IND
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