Preparation method for selenium-enriched glutathione beer yeast biological product by utilizing waste beer yeast

A technology of glutathione and biological products, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of high production cost of selenium-enriched products

Inactive Publication Date: 2014-07-23
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] In view of the high production cost of selenium-enriched products produced by fermentation of pure-bred microorganisms, the purpose of the present invention is to provide a method for preparing selenium-enriched glutathione yeast using beer waste yeast

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Dissolve 5.0 g of phosphoric acid, 4.0 g of ammonium sulfate, 1.8 g of magnesium sulfate, 0.15 g of calcium chloride, 9.0 mg of sodium selenite and 30 g of glucose into 400 mL of tap water, adjust the pH to 5.0, and place in a 2000 mL Erlenmeyer flask 115 °C, high-pressure damp heat sterilization for 30 min; Dissolve 0.1 g glutamic acid, 0.05 g glycine, and 0.075 g cysteine ​​in 100 mL distilled water, filter and sterilize with a 0.22 μm filter membrane, and combine into a container In a 2000 mL Erlenmeyer flask of sterilized culture medium. Add 100 g of fresh yeast sludge that has been washed and centrifuged, and incubate at 28±1°C and 150 rpm for 18 h.

[0026] The results showed that the selenium content in yeast was determined by conventional methods to be 1101 μg / g yeast, of which the organic selenium content accounted for 90%, the selenoprotein content accounted for 78.6%, and the glutathione content per gram of selenium-enriched glutathione dry yeast was about 6....

Embodiment 2

[0029] Dissolve 50.0 g of phosphoric acid, 13.4 g of ammonium sulfate, 20 g of magnesium sulfate, 2 g of calcium chloride, 33.4 mg of sodium selenite and 300.0 g of glucose into 4900 mL of tap water, adjust the pH to 5.0, and place in a 10 L fermenter 115 °C, high-pressure damp heat sterilization for 20 min; Dissolve 10 g of glutamic acid, 7.5 g of cysteine, and 5 g of glycine in 100 mL of distilled water, filter and sterilize with a 0.22 μm filter membrane, and put them into a container in sterilized culture medium. Add 1250 g of fresh yeast sludge that has been washed and centrifuged, the temperature is 28±1°C, the stirring speed is 200-500 rpm, and the dissolved oxygen is kept greater than 25%, and the transformation is carried out for 19 hours.

[0030] The results showed that the selenium content in yeast was determined by conventional methods to be 1223 μg / g yeast, of which organic selenium content accounted for 91.0%, selenoprotein content accounted for 77.8%, and gluta...

Embodiment 3

[0033] Dissolve 150.0 g of phosphoric acid, 40 g of ammonium sulfate, 50 g of magnesium sulfate, 5.5 g of calcium chloride, 100 mg of sodium selenite, and 900.0 g of glucose into 14 L of tap water, adjust the pH to 5.0, and place in a 30 L fermenter 115 °C, high-pressure damp heat sterilization for 25 min; dissolve 35 g of glutamic acid, 25 g of cysteine, and 15 g of glycine in 1L of distilled water, filter and sterilize with a 0.22 μm filter membrane, and put them into a well-preserved in sterile culture medium. Add 4000 g of fresh yeast sludge that has been washed and centrifuged, the temperature is 29±1°C, the stirring speed is 200-500 rpm, and the dissolved oxygen is kept greater than 30%, and the culture is transformed for 17 hours.

[0034] The results showed that the selenium content in yeast was determined by conventional methods to be 1424 μg / g yeast, of which organic selenium content accounted for 90%, selenoprotein content accounted for 80.1%, and glutathione conten...

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Abstract

A selenium-enriched glutathione beer yeast biological product is characterized in that waste beer yeast is used for transforming inorganic selenium into organic selenium, and glutathione precursor amino acids are used for synthetizing glutathione, and thus the selenium-enriched glutathione yeast is obtained. The beer yeast biological product provided by the invention not only realizes the recycling of industrial waste of beer, but the glutathione also can promote the activity of the yeast selenium, which makes up deficiency of single selenium enriching in the existing products. The selenium-enriched glutathione beer yeast biological product not only has the effects of oxidation resistance, enhancement of human immunity and so on; at the same time, the selenium-enriched glutathione beer yeast biological product is a high efficient nutrient enhancer and supplement by providing a plurality of amino acids and vitamins to the human body. A preparation method for the selenium-enriched glutathione beer yeast biological product is characterized in that the yeast liquid biotransformed by the organic selenium and the glutathione is subjected to centrifuging, cleaning, heavy suspending, high-pressure homogenizing, colloidal milling, cold drying, pelletizing and tablet compressing to obtain the swallowable or chewable selenium-enriched glutathione yeast tablets. The selenium-enriched glutathione yeast tablets have the advantages of being convenient to eat, stable in components and easy to preserve.

Description

technical field [0001] The invention relates to a method for preparing selenium-enriched glutathione yeast biological products by utilizing fresh beer waste yeast to carry out biotransformation of sodium selenite to synthesize organic selenium, simultaneously transform glutathione precursor amino acid to synthesize glutathione. Background technique [0002] Selenium is one of the essential trace elements for humans and livestock. It is generally believed that the biological function of selenium mainly comes from selenoprotein (selenoprotein), and the biosynthesis of selenoprotein is encoded by the codon UGA on the mRNA selenocysteine (Selenocysteine, Sec) combines selenium into proteins by covalent bonds, so the biosynthesis and metabolic mechanism of selenoproteins have important theoretical value for molecular biology and biochemistry. Numerous studies have shown that selenoproteins play important roles in antioxidant activity, regulation of redox signaling, regulation of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/304A23L1/305A23K1/16C12N1/18C12R1/865A23K10/16A23L33/16A23L33/175
CPCA23L33/16A23K50/80A23L33/14A23L33/175A23P10/28A23V2002/00A23V2200/324A23V2200/30A23V2250/762
Inventor 孙纳新叶春江刘月辉张桂香杨新超王元秀
Owner UNIV OF JINAN
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