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Mammary cancer diversion and prognosis molecule parting gene group, gene chip producing and using method

A gene chip and molecular typing technology, which is applied in the fields of hybridization and analysis, functional genomics and gene chip preparation, can solve the problems of inability to predict patients with high metastatic potential, shortened metastasis-free survival and overall survival, and patients who lose comprehensive treatment Opportunity and other issues

Active Publication Date: 2008-05-07
TIANJIN MEDICAL UNIV CANCER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the former group of patients, the current clinical routine prognostic indicators can predict the prognosis of patients more accurately, and various treatment strategies at home and abroad are also aimed at this part of the patients; but for the latter group of patients, due to the clinical routine The prognostic indicators of the disease cannot predict the high metastatic potential of patients, so these patients often lose the favorable opportunity for comprehensive treatment, and the metastasis-free survival and overall survival are greatly shortened
[0004] The acquisition of the metastatic phenotype of tissue cells is based on the genotype changes involved in the biological process of metastasis. The complexity of the biological process of metastasis and the heterogeneity of breast cancer tissue cell growth determine that this genotype is not a single gene or Abnormal changes in a few genes, so the currently known metastasis prognosis related genes c-erbB-2, p53, etc. cannot predict the prognosis of clinical patients

Method used

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  • Mammary cancer diversion and prognosis molecule parting gene group, gene chip producing and using method
  • Mammary cancer diversion and prognosis molecule parting gene group, gene chip producing and using method
  • Mammary cancer diversion and prognosis molecule parting gene group, gene chip producing and using method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1. Screening and Application of Lymph Node Positive Breast Cancer Prognosis Prediction Gene Group

[0020] material method

[0021] 1. Case selection and specimen processing

[0022] All specimens were collected from breast cancer patients with positive lymph node metastasis who received radical mastectomy from January 2002 to December 2003 in the Cancer Hospital Affiliated to Tianjin Medical University. lymph nodes. Samples of primary breast cancer and lymph node metastases were taken from each case. HE staining of histological sections confirmed that cancer cells accounted for more than 75% of lymph node metastases. The tissue samples were quickly frozen in liquid nitrogen and stored at -80°C. 26 cases of lymph node positive breast cancer were used as training cases (training cases), with an average follow-up of 43 months, 11 of which developed distant metastasis; the other 9 cases of lymph node positive breast cancer were used as verification cases (test ca...

Embodiment 2

[0052] Example 2, Breast cancer lymph node metastasis diagnosis gene group screening and application

[0053] Materials and methods

[0054] 1. Case selection and specimen processing

[0055] The primary cancer tissues of 49 cases of breast cancer in this group were all obtained from breast cancer patients who underwent radical mastectomy from January 2002 to December 2003 in the Department of Breast Cancer Hospital Affiliated to Tianjin Medical University. Invasive ductal carcinoma (WHO classification), the average follow-up time was 43 months. 10 lymph node-negative breast cancer patients and 10 lymph node-positive breast cancer patients were randomly selected as training cases, and 19 lymph node-negative patients and 10 lymph node-positive breast cancer patients were selected as verification cases. The case data are shown in Table 5. In addition, 32 normal mammary gland tissues of patients with breast tumors were extracted and mixed in equal amounts as control samples. A...

Embodiment 3

[0076] Example 3. Screening and Application of Lymph Node Negative Breast Cancer Metastasis Prediction Gene Group

[0077] Breast cancer has the biological characteristics of tissue cell heterogeneity. During the process of tumor cell occurrence, progression and metastasis, there are not only quantitative accumulation of clonal growth at the primary site, but also the acquisition of cells with high metastatic potential that are accompanied by selective growth at any time. Without the ability to escape and disseminate, secondary metastases develop in the axillary lymph nodes and distant organs. Therefore, some metastatic cancers are the so-called "heterogeneous metastasis" that occurs in the late stage of tumor progression, but it may also be the so-called "homogeneous metastasis" that occurs in the early stage of tumor development. In the first part of this study, 79 genes were screened to have a common difference trend between multiple cases of primary breast cancer and paire...

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Abstract

The invention discloses three gene groups for transferring and typing prognostic numerator to breast cancer, a gene chip, an unsupervised clustering map from a gene expression profile and a method for transferring and typing prognostic numerator to breast cancer patients. The invention is characterized in that: the gene chip is point-sampled and prepared by specific cDNA or oligonucleotide of the gene groups with an lattice form on the surface of the supports, and is used for transferring and typing prognostic numerator to breast cancer; the cDNA or amplified products of primary cancer tissue specimens based on breast cancer patients are fluorescence-marked and then crossbred with the gene chip for transferring and typing prognostic numerator to breast cancer, and then the gene expression profile is obtained, and the unsupervised clustering map for transferring and typing prognostic numerator to patients comes from the gene expression profile; the method makes use of the gene chip and the gene expression profile for transferring and typing prognostic numerator to breast cancer.

Description

technical field [0001] The invention relates to functional genomics and gene chip preparation, hybridization and analysis techniques. Specifically, the gene group that can be used for breast cancer metastasis and prognosis molecular typing is screened within the range of human functional genome expression profile, and prepared into a gene chip, which is applied to the metastasis and prognosis prediction of breast cancer patients. Background technique [0002] Breast cancer is one of the most common malignant tumors in women. In recent years, the incidence of breast cancer has been increasing year by year in my country, and it is showing a younger trend, becoming a major threat to women's health. Despite the continuous improvement of clinical diagnosis and treatment measures, the mortality rate of breast cancer patients is still not effectively controlled, and recurrence and metastasis are the main causes of death in breast cancer patients. The key to improving the survival ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11C12N15/12
Inventor 郝希山孙保存冯玉梅李晓青张微
Owner TIANJIN MEDICAL UNIV CANCER HOSPITAL
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