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Relative transferring function of kinesin family member KIF1B, landmark application thereof in predicting tumor patient prognosis and application method thereof

A technology for kinesin and tumor patients, applied in biochemical equipment and methods, microbial determination/inspection, measuring devices, etc.

Inactive Publication Date: 2010-10-06
TIANJIN MEDICAL UNIV CANCER HOSPITAL
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Problems solved by technology

[0005] At present, the research reports on KIF1B are all about molecular structure and biological function based on molecular structure, but the impact of KIF1B gene changes on cell biological behavior, relationship with histopathological changes, and gene expression detection methods and applied research is still blank

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  • Relative transferring function of kinesin family member KIF1B, landmark application thereof in predicting tumor patient prognosis and application method thereof
  • Relative transferring function of kinesin family member KIF1B, landmark application thereof in predicting tumor patient prognosis and application method thereof
  • Relative transferring function of kinesin family member KIF1B, landmark application thereof in predicting tumor patient prognosis and application method thereof

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[0020] 1 Materials and methods

[0021] 1.1 Case data and specimen collection 180 cases of primary breast cancer were collected from surgical resection specimens of breast cancer patients admitted to the Department of Breast Cancer Hospital Affiliated to Tianjin Medical University from May 1995 to May 2004, and all specimens were obtained with the informed consent of the patients. All patients had no preoperative chemotherapy, and the pathological types were invasive ductal carcinoma (WHO classification). The follow-up time ranged from 36 to 100 months, and 180 patients were followed up for more than 3 years, of which 132 patients were followed up for more than 5 years, with a median follow-up time of 62 months; 4 cases relapsed and 39 cases developed distant metastasis. All specimens were confirmed by histopathology. The instruments used for tissue specimen collection were all RNase-free, and the specimens were quick-frozen in liquid nitrogen and stored in a -80°C refrigerat...

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Abstract

The invention discloses a relative transferring function of kinesin family member KIF1B, a landmark application thereof in predicting tumor patient prognosis and an application method thereof, belonging to the technical field of tumor molecular biology. The invention discloses a relative transferring function of kinesin family member KIF1B, a landmark application of KIF1B expression level in predicting tumor patient prognosis and a detection and application method based on the KIF1B expression level in predicting tumor patient prognosis. In the method, real-time quantitative RT-PCR is carried out to detect the method for KIF1B mRNA expression level; quantitative PCR amplification comprises relative quantitative PCR amplification and absolute quantitative PCR amplification; the KIF1B expression level detection can also adopt a molecular hybridization method to detect the KIF1B mRNA expression level; an immunostaining method and / or immunoblotting method can be adopted to detect the KIF1B protein expression level; a critical value for prognosis judgment is determined according to the KIF1B gene or protein expression level in the primary cancer tissues of recurrent and metastatic positive cases and recurrent and metastatic negative cases of tumor patients; and the patient the critical value of whom is higher than the threshold value has favorable prognosis, and the patient the critical value of whom is lower than the threshold value has poor prognosis.

Description

technical field [0001] This project belongs to the invention of new functions, new uses and application methods of known genes. Specifically, a new function related to tumor metastasis of the known gene KIF1B was discovered, which can be used as a molecular marker and therapeutic target for predicting the prognosis of tumor patients; a method for predicting the prognosis of breast cancer patients based on the expression levels of KIF1B gene mRNA and protein was disclosed . technical background [0002] Distant organ metastasis caused by hematogenous dissemination of primary tumor cells in patients with malignant tumors is the key to treatment failure and death in cancer patients. The discovery of tumor metastasis-related genes can provide clues for the study of tumor metastasis mechanisms, molecular markers for early diagnosis of metastasis, objective indications for patient prognosis assessment, and molecular targets for anti-metastasis therapy. [0003] Kinesin-like prot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/16C12Q1/68G01N33/573
Inventor 冯玉梅李林李晓青郝希山
Owner TIANJIN MEDICAL UNIV CANCER HOSPITAL
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