Infectious bursal virus and method for propagating bursal virus with chicken embryo cell line and bioreactor to prepare inactivated vaccine and combined vaccine

Abstract

The invention relates to an infectious bursal disease virus (IBDV) HQ strain CGMCC NO.4935 and a preparation method of inactivated vaccines and combined vaccines for infectious bursal disease (IBD). The preparation method mainly comprises the following steps of: (1) carrying out chain amplification on cell seeds; (2) adding cell growth-promoting liquid into a sterilized bioreactor, and inoculating cells for preparing vaccines for suspension culture; (3) replacing maintenance liquid containing an IBDV strain when cells are of the maximum density, and continuing to culture; (4) collecting viruses in time, and measuring virus titer; and (5) inactivating virus liquid, and preparing inactivated vaccines and combined vaccines thereof for the IBD according to different proportions. The method provided by the invention increases the cell density, improves the virus titer, improves the vaccine titer, reduces the side reaction, reduces the labor intensity, lowers the production cost, improves the controllability of production processes, and ensures the uniformity and stability of product quality. The produced inactivated vaccines and combined vaccines thereof for the IBD have the advantagesof good safety and high immune efficiency and have a complete protective effect on the IBDV attack.

Description

technical field [0001] The invention relates to the technical field of veterinary biological products, in particular to an infectious bursal disease virus and a method for preparing an inactivated vaccine and a combined vaccine by propagating the bursal disease virus with chicken embryo cell lines and a bioreactor. Background technique [0002] Infectious Bursal Disease Virus (IBDV) belongs to the double-stranded RNA virus family and belongs to the double-stranded RNA virus genus. The pathogen mainly destroys the central immune organ of chickens - the bursa of Fabricius, causing the body's immunosuppression, leading to the death of infected chicks and the increase of infectivity to (other) pathogenic factors, and the decline of immune response to (other) vaccines. cause serious harm. At present, the disease is mainly prevented and controlled through vaccination. In addition to immunizing chicks with live vaccines, breeders are usually injected with inactivated vaccines to ...

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Problems solved by technology

The spinner bottle primary cell (chicken embryo fibroblast) culture method is that the cultured cells adhere to the wall and grow on the inner wall of the glass culture flask. Although the operation technology requirements are low and the technology is mature and stable, it is not suitable for the current large-scale animal vaccine production. , mainly as follows: (1) the proliferation titer of infectious bursal disease virus on chicken embryo fibroblasts is not high, and it is necessary to increase the concentration multiple and increase the production cost, so the application effect is poor in practice; (2) the transfection Bottled chicken embryo fibroblast culture cannot adjust the culture conditions in a timely manner, and cannot ensure that the cell culture is in the best condition, so the cultured cell density is small, the titer of virus reproduction is low, the effect of chicken immunization is poor, and the side effects are large; (3) The production process of spinner bottle cultured cells is not completely controllable, the difference between cell batches is large, and the product quality is uneven and unstable; (4) The labor intensity of spinner bottle cultured cells is high and the production efficiency is low; (the virus liquid needs to be increased Qualified vaccines can only be made after concentration multiples, and the production cost is high;) (5) There is a biological safety hazard of chicken embryo source exogenous virus contamination

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