Bama miniature pig somatic cell cloning method

A technology of somatic cell cloning and Bama pig, which is applied in the field of animal embryo biology and animal reproduction, can solve the problems of not finding the same or similar reports, etc.

Inactive Publication Date: 2010-10-13
GUANGXI UNIV
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  • Abstract
  • Description
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Problems solved by technology

[0002] Have not found so far identical or similar report with the present invention

Method used

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  • Bama miniature pig somatic cell cloning method

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Experimental program
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Embodiment

[0072] 1. Collection and In Vitro Maturation of Porcine Oocytes

[0073] Common commercial pig ovaries were collected from local slaughterhouses, placed in 0.9% saline at 30-37°C, transported back to the laboratory within 5 hours, and washed with saline for 3-5 times. Use a 10ml syringe with a 12-gauge needle to extract oocytes with a diameter of 2-6ml follicles on the surface of the ovary, and use a glass pipette to absorb oocytes (COCs) with more than 2 layers of cumulus cells and uniform cytoplasm under a solid microscope. Wash 3 times in egg wash and 2 times in maturation solution. Move the oocytes into the mature liquid droplet equilibrated in the incubator for at least 4h, cover with embryo grade mineral oil, culture for 24h, and then transfer to the mature liquid drop without FSH and LH to continue culturing for 18-20h. The incubator conditions are: 5% CO2 air, maximum relative saturated humidity, 39°C. Then cumulus cells were removed with 0.1% hyaluronidase, and the ...

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Abstract

The invention discloses a Bama miniature pig somatic cell cloning method, which comprises the following steps: 1) preparing related agents; 2) cultivating Bama miniature pig somatic cells; 3) freezing the Bama miniature pig somatic cells; 4) unfreezing the frozen somatic cells; 5) acquiring and externally cultivating pig oocytes; 6) transplanting nucleus; and 7) transplanting embryos. The invention has the advantages of speeding up the research on domestic transgenosis pig technology and human heterogeneous organ transplantation, accelerating the development of animal husbandry in Guangxi even the whole country, and academically filling the gap in China.

Description

technical field [0001] The invention relates to animal reproduction and animal embryo biotechnology, in particular to a method for somatic cell cloning of Bamaxiang pigs. Background technique [0002] So far, no report identical or similar to the present invention has been found. Contents of the invention [0003] The purpose of the invention is to: [0004] 1. Solve the key technical problems necessary for the research of transgenic pigs in human xenotransplantation, and lay the basic and necessary research methods for the research of gene-targeted pigs and xenotransplantation. [0005] 2. It opens up a new way for the preservation of the excellent local breed Bamaxiang pig with important economic value. It can also breed other breeding pigs with excellent traits in large quantities, and solve the problems of introduction, conservation and variety improvement of excellent breeding pigs, greatly greatly Speed ​​up the introduction, conservation and variety improvement of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/877A61D19/04A01K67/027
Inventor 卢晟盛卢克焕刘红波胡林林
Owner GUANGXI UNIV
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