Deer X/Y sperm separation freezing sperm and producing method thereof and use

Abstract

The invention relates to a frozen semen deer X / Y sperm separation and a production method and application thereof. The invention provides a frozen semen deer X / Y sperm separation, comprising frozen equilibrium liquid A, frozen equilibrium liquid B containing 12% of glycerin cryoprotectant and frozen semen of deer X or Y sperm separation. The invention uses TALP liquid for protecting the fresh sperms of deer, and applies the difference of DNA contents with deer X and Y sperms to separate X and Y sperms by a flow cytometry mode, so that frozen semen of sexual control is produced in the frozen conserving conditions of -90 DEG C and 60 minutes of processing. The frozen semen obtained by the invention is used for controlling the sex of calved deer in the way of artificial insemination or embryo transplantation with high separation efficiency, and the accuracy of sexual control can be more than 88%. The invention can be applied to industrialized production and cultivation, thereby greatly improving the cultivation effects of deer.

Description

technical field

[0001] The invention relates to a mammal X / Y sperm separation technology, in particular to a deer X / Y sperm separation frozen semen and its production method and application. Background technique

[0002] Livestock-based frozen semen, artificial insemination and in vitro fertilization techniques are widely used in production breeding and reproductive biology research. Under normal circumstances, the male and female offspring of deer breed each account for 50%, but in actual production, in order to improve the breeding effect of deer, more breeding doe (breeding and group expansion) or male deer (commodity) are required for specific strains. deer antler production), so it is necessary to control the sex of deer offspring by separating sperm. In addition, separated sperm usually need to be cryopreserved before insemination, and different species have different requirements for pH value, freezing buffer and freezing treatment method, which puts higher demands o...