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Deer X/Y sperm separation freezing sperm and producing method thereof and use

A technology for freezing semen and production methods, which is applied in the directions of biochemical equipment and methods, applications, microorganisms, etc., can solve the problem that the number and vitality of deer sperm cannot reach the ideal industrial application, etc., and achieves protection of sperm vitality, high separation efficiency, and improved The effect of the breeding effect

Active Publication Date: 2008-12-17
内蒙古赛科星繁育生物技术(集团)股份有限公司
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

This method needs to add the semen of different kinds of domestic animals. When using this method to process the sperm of deer alone, the quantity and vitality of the obtained deer sperm cannot reach the level of ideal industrial application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Production of deer Y frozen semen and artificial insemination to control calving sex:

[0036] 1) After anesthetized from a healthy red deer (Diamond No. 1), 4 mL of fresh semen with a total sperm count of 4 billion was collected by electrical stimulation. After testing, the sperm motility was 83%. Since deer's fresh essence is very easy to lose vitality, it needs to be protected with TALP solution. Equally dilute with TALP solution and add antibiotics (Gentamicin: 0.8mg / mL, Linco-spectromycin: 0.3mg / mL) and then incubate at 18-20°C. The concentration and motility of the fresh semen were tested using a sperm density-motility meter. After testing, the motility of the semen was 85%, and it was transported back to the laboratory for 6 hours of X / Y sperm separation.

[0037] 2) According to the converted volume of semen concentration, take 4×200 million sperm (for 2 sperm separators) and centrifuge to remove the seminal plasma, add 10 μg / mL Hochest 33342 and stain in a war...

Embodiment 2

[0056] Production of deer X frozen semen and artificial insemination to control calving sex:

[0057] 1) After being anesthetized from a healthy red deer (Diamond No. 3), 3 mL of fresh semen with a total sperm count of 2.4 billion was collected by electrical stimulation. After testing, the sperm motility was 80%. Since deer's fresh essence is very easy to lose vitality, it needs to be protected with TALP solution. Equally dilute with TALP solution and add antibiotics (Gentamicin: 0.8 mg / mL, Linco-spectromycin: 0.3 mg / mL) and then incubate at 18-20°C. The concentration and motility of the fresh semen were tested with a sperm density-motility meter. After testing, the motility of the semen was 85%, and it was transported back to the laboratory for 4 hours of X / Y sperm separation.

[0058] 2) According to the converted volume of semen concentration, take 4×200 million sperm (for use by 2 sperm separators) and centrifuge to remove the seminal plasma, add 10 μg / mL Hochest33342 and...

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PUM

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Abstract

The invention relates to a frozen semen deer X / Y sperm separation and a production method and application thereof. The invention provides a frozen semen deer X / Y sperm separation, comprising frozen equilibrium liquid A, frozen equilibrium liquid B containing 12% of glycerin cryoprotectant and frozen semen of deer X or Y sperm separation. The invention uses TALP liquid for protecting the fresh sperms of deer, and applies the difference of DNA contents with deer X and Y sperms to separate X and Y sperms by a flow cytometry mode, so that frozen semen of sexual control is produced in the frozen conserving conditions of -90 DEG C and 60 minutes of processing. The frozen semen obtained by the invention is used for controlling the sex of calved deer in the way of artificial insemination or embryo transplantation with high separation efficiency, and the accuracy of sexual control can be more than 88%. The invention can be applied to industrialized production and cultivation, thereby greatly improving the cultivation effects of deer.

Description

technical field [0001] The invention relates to a mammal X / Y sperm separation technology, in particular to a deer X / Y sperm separation frozen semen and its production method and application. Background technique [0002] Livestock-based frozen semen, artificial insemination and in vitro fertilization techniques are widely used in production breeding and reproductive biology research. Under normal circumstances, the male and female offspring of deer breed each account for 50%, but in actual production, in order to improve the breeding effect of deer, more breeding doe (breeding and group expansion) or male deer (commodity) are required for specific strains. deer antler production), so it is necessary to control the sex of deer offspring by separating sperm. In addition, separated sperm usually need to be cryopreserved before insemination, and different species have different requirements for pH value, freezing buffer and freezing treatment method, which puts higher demands o...

Claims

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Application Information

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IPC IPC(8): A01N1/02C12N5/06A61D19/00C12N5/076
Inventor 李喜和王建国钱松晋董云祥刘树江胡树香孙伟
Owner 内蒙古赛科星繁育生物技术(集团)股份有限公司
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