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Application of human mature follicle fluid in external mature technology of immature ovum

A technology of mature follicles and in vitro maturation, applied in the field of assisted reproduction, can solve the problems that there is no in vitro maturation culture medium for immature eggs, and achieve the effect of easy source

Inactive Publication Date: 2002-12-18
JIANGSU PROVINCE HOSPITAL
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

At present, there are various reports of in vitro maturation culture medium, but there is no relatively ideal and stable commercial immature egg in vitro maturation culture medium.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0011] 1. The patient undergoes slight preparation for ovulation induction and endometrium preparation. When the average follicle diameter is 6-12mm under B-ultrasound monitoring, immature eggs are obtained through vaginal puncture under B-ultrasound.

[0012] 2. The immature eggs are taken out, in the culture fluid containing mature follicular fluid prepared by the present invention, at 37 ° C, 5% CO 2 The cumulus granulosa cells were removed for 36-48 hours, and the eggs were scored for maturity. The maturity rate of immature eggs was 68.4%.

[0013] 3. After the husband’s sperm is obtained, the mature eggs showing the first polar body maturation mark will be fertilized in vitro according to the method of single sperm intracytoplasmic injection. Embryos of -8 cells are implanted into the mother's uterus.

[0014] 4. The mother's endometrium is prepared synchronously according to the stage of egg maturation and fertilization, accepts embryo implantation, and maintains corpus...

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Abstract

The present invention belongs to the field of auxiliary reproduction technology. Said invention described culture solution containing human mature follicular fluid is used for external culture technique of human immature ovum, and its composition is formed from Ham'e F-10 or TCM199, human serum substitute SSS, rFSH, HCG, 17 beta-estradiol and human mature follicular fluid. The in-vitro mature (IVM) technique of immature ovum can be used to take out the immature ovum of sterility woman with ovum maturation disturbance, and the culture solution prepared by said invention is used to culture it, and make it mature and make in-vitro fertilization and embryo transplantation to help gestation. It can obtain 65% of ovum mature rate and 33.3% of gestatino rate.

Description

1. Technical field [0001] The invention belongs to the technical field of assisted reproduction. 2. Background technology [0002] In-vitro maturation (IVM) of immature eggs is a cutting-edge technology in assisted reproductive technology in the field of reproductive medicine. Since the birth of the world's first "test-tube baby" in 1978, assisted reproductive technology and a series of related derivative technologies have developed rapidly, helping many infertile couples to obtain fertility. However, some of these women cannot obtain normal mature eggs for various reasons, and conventional assisted reproductive technology cannot do anything about it. The in vitro maturation (IVM) technology of immature eggs is to take out the immature eggs of the nuclear vesicle stage (GV stage) in women's ovaries, and use a special culture medium to simulate the microenvironment of the follicles in the body. Micro-insemination is then carried out, and after developing into embryos, they ...

Claims

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Application Information

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IPC IPC(8): A61B17/425C12N5/075
Inventor 钱云刘嘉茵冒韵东王淑玉丁卫杨乃明
Owner JIANGSU PROVINCE HOSPITAL
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