Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Equus oocyte vitrification cryopreservation fluid, cryopreservation method and application

A vitrification and oocyte technology, applied in the field of equine oocyte vitrification and cryopreservation solution, can solve the problems of chemical and physical damage

Active Publication Date: 2017-10-10
QINGDAO DERUI JUNFA BIOLOGY TECH +1
View PDF4 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of chemical and physical damage caused by ice crystals to cells and the toxicity of freezing liquid to cells during cryopreservation of equine oocytes and embryos, and improve the recovery rate of oocytes or embryos, the invention provides an equine animal Oocyte vitrification cryopreservation solution and its preparation method, cryopreservation method and application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 Freezing solution one

[0039] An embodiment of the present invention provides a vitrified cryopreservation solution for equine animal oocytes, including three liquids: base solution (BS), equilibrium solution (ES) and vitrification solution (VS); wherein,

[0040] Base solution (BS) is composed of inactivated fetal bovine serum with a volume fraction of 20% and a HEPES solution with a volume fraction of 80% and a concentration of 10mmol / L; the preparation method is: in a sterile environment, take inactivated Fetal calf serum (NCS) was added to the HEPES solution, dissolved and shaken to obtain 10ml of base solution (BS).

[0041] Equilibrium solution (ES) is composed of the following volume percentage components: 8% ethylene glycol (EG), 4% dimethyl sulfoxide (DMSO), 4% propylene glycol (PROH), 20% inactivated fetal calf serum (NCS ), 5% carboxylated ε-polylysine (COOL-PLL), and the rest is 10mmol / L HEPES solution; each liter of equilibrium solution (ES) ...

Embodiment 2

[0044] Example 2 Refrigerant II

[0045] An embodiment of the present invention provides a vitrified cryopreservation solution for equine animal oocytes, including three liquids: base solution (BS), equilibrium solution (ES) and vitrification solution (VS); wherein,

[0046] Base solution (BS) is composed of inactivated fetal bovine serum with a volume fraction of 20% and a HEPES solution with a volume fraction of 80% and a concentration of 10mmol / L; the preparation method is: in a sterile environment, take inactivated Fetal calf serum (NCS) was added to the HEPES solution, dissolved and shaken to obtain 10ml of base solution (BS).

[0047] Equilibrium solution (ES) is composed of the following volume percentage components: 8% ethylene glycol (EG), 4% dimethyl sulfoxide (DMSO), 4% propylene glycol (PROH), 20% inactivated fetal calf serum (NCS ), 5% carboxylated ε-polylysine (COOL-PLL), and the rest is 10mmol / L HEPES solution; each liter of equilibrium solution (ES) also inclu...

Embodiment 3

[0050] Example 3 Freezing solution three

[0051] An embodiment of the present invention provides a vitrified cryopreservation solution for equine animal oocytes, including three liquids: base solution (BS), equilibrium solution (ES) and vitrification solution (VS); wherein,

[0052] Base solution (BS) is composed of inactivated fetal bovine serum with a volume fraction of 20% and a HEPES solution with a volume fraction of 80% and a concentration of 10mmol / L; the preparation method is: in a sterile environment, take inactivated Fetal calf serum (NCS) was added to the HEPES solution, dissolved and shaken to obtain 10ml of base solution (BS).

[0053] Equilibrium solution (ES) is composed of the following volume percentage components: 8% ethylene glycol (EG), 4% dimethyl sulfoxide (DMSO), 4% propylene glycol (PROH), 20% inactivated fetal calf serum (NCS ), 5% carboxylated ε-polylysine (COOL-PLL), and the rest is 10mmol / L HEPES solution; each liter of equilibrium solution (ES) a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses an equus oocyte vitrification cryopreservation fluid, a cryopreservation method and an application. The equus oocyte vitrification cryopreservation fluid comprises a base solution (BS), an equilibrium solution (ES) and a vitrification solution (VS), wherein the VS is composed of the following components by volume percent: 10-20% of glycol (EG), 6-10% of dimethyl sulfoxide (DMSO), 6-10% of propylene glycol (PROH), 15-25% of inactivated fetal calf serum (NCS), 8-15% of carboxylate E-polylysine (COOL-PLL) and the balance of an HEPES solution. Each liter of the VS includes 0.3-0.8mol of saccharose. The invention has the beneficial effects that the vitrification cryopreservation fluid has low cytotoxicity, high permeability and obvious vitrification effect, meanwhile can obviously reduce the generation of ice crystals in freezing and re-warming processes, can protect cells from physical injury, can increase the survival rate of oocyte, is suitable for the vitrification cryopreservation of equus oocytes or embryos of horses, donkeys and the like, and is beneficial to the popularization and application of artificial insemination and embryo transplantation technology.

Description

technical field [0001] The invention belongs to the technical field of cryopreservation of animal oocytes and embryos, and in particular relates to a vitrified cryopreservation solution, a cryopreservation method and application of equine animal oocytes. Background technique [0002] Oocyte cryopreservation refers to the preservation of collected oocytes under ultra-low temperature (-196°C liquid nitrogen) conditions, so that their metabolic activities are stopped and they are in a dormant state. When the external environment reaches suitable conditions, they can continue to fertilize and develop into healthy oocytes. A preservation technique for embryos. Oocyte cryopreservation technology can provide conditions for the establishment of oocyte banks or gene banks of fine varieties; it can maintain various animal varieties, strains and rare and endangered animals lost due to diseases or other reasons; it can realize simple and cheap long-distance transportation , to facilita...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 李莎莎邹敬清邹志钢荣美洁王浩李成修
Owner QINGDAO DERUI JUNFA BIOLOGY TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com