Rapid snake wound identification method

A snakebite, rapid technology, applied in the field of snakebite identification, can solve the problems of lack of rapid, simple and effective diagnostic methods, and achieve the effects of short detection time, small volume and low preparation cost

Inactive Publication Date: 2012-07-18
高建芳 +4
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, the judgment of the type of snakebite in China still relies on its clinical symptoms, and there is a lack of fast, simple and effective diagnostic methods for clinical auxiliary judgment of snakebite

Method used

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  • Rapid snake wound identification method
  • Rapid snake wound identification method
  • Rapid snake wound identification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0027] 1. Preparation of anti-venom immunoglobulin (IgG). Select 6 kinds of snake venoms to be identified (cobra, coral snake, five-step snake, Agkistrodon halys, bamboo leaf green and iron head snake venom), dissolve them in 20mM PBS, centrifuge to get the supernatant, and determine the protein content. Rats were injected subcutaneously at multiple points to prepare six kinds of polyclonal antivenom crude serum. The crude serum was firstly precipitated by ammonium sulfate to remove non-protein components, and then purified by Hitrap protein IgG column to obtain 6 kinds of anti-venom IgG.

[0028] 2. Screening of snake venom species-specific IgG. The snake venom was coupled to the activated CNBr-activated sepharose 4B affinity medium, and 0.3 g of wet affinity medium coupled with 5 kinds of snake venoms were randomly taken in equal amounts, mixed and put into the inner tube of the centrifugal concentration tube. At room temperature, 500 μg of antiserum IgG of each snake veno...

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Abstract

The invention discloses a rapid snake wound identification method using an enzyme mark strip as a carrier, which includes that an enzyme mark hole is coated by a snake venom species specific antibody, blood serum collected from a patient with a snake wound is dropped in the hole, and snake wound types can be judged in a short time. Screening of various specific antibodies and preparation of the enzyme mark strip and auxiliary reagents in the testing process are low in cost; a testing process is simple without the help of instruments, and testers do not need abundant clinical diagnosis experience; testing time is short, and the time from taking out the enzyme mark strip under low temperature to result developing is about 40-45 minutes; accuracy is high, an AB-double-antibody sandwich method can effectively guarantee combination of snake venom and specific antibodies, biotin-avidin amplifies signals effectively, and developing results can be observed directly through naked eyes.

Description

Technical field [0001] The invention belongs to a snakebite identification method, in particular to a method for rapidly identifying snakebite by using an enzyme-marked strip coated with snake venom-specific antibodies. Background technique [0002] Traditionally, the judgment of the type of snakebite is based on the analysis of the clinical symptoms of snakebite. Due to the lack of experience of doctors, the clinical symptoms are not obvious or have spread to the point where it cannot be judged, it will lead to misjudgment and delay the treatment of snakebite. In order to avoid the defects of traditional snakebite identification, some countries around the world have carried out research on the preparation of snakebite rapid diagnostic products with snake venom-specific antibodies. The relevant diagnostic products can be accurately detected in a short period of time by using the body fluids of patients bitten by snakes. To accurately determine the type of snakebite, so as to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
Inventor 高建芳王金屈彦福马小梅计翔
Owner 高建芳
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