Fine purification method of polyvalent anti-snake venom freeze-dry blood serum

An anti-snake venom and freeze-drying technology, which is applied in the direction of antibodies, skin diseases, and drug combinations, can solve the problems of low purity and low antibody titer, and achieve the effects of low foreign protein, good drug safety, and improved purity

Inactive Publication Date: 2009-03-18
KUNMING INST OF MILITARY MEDICINE CHENGDU MILITARY REGIONS CENT FOR DISEASE CONTROL & PREVENTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the product still has problems such as low purity, low antibody titer, and no freeze-drying process.

Method used

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  • Fine purification method of polyvalent anti-snake venom freeze-dry blood serum
  • Fine purification method of polyvalent anti-snake venom freeze-dry blood serum
  • Fine purification method of polyvalent anti-snake venom freeze-dry blood serum

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] (1) Preparation of antiserum of the present invention

[0021] 1. Preparation of antigen

[0022] The venoms of five-step snake, pit viper, iron head, bamboo leaf green, viper, coral snake, golden krait, cobra, and king cobra were obtained by live snake bite and extrusion method respectively. The fresh venom is immediately vacuum-dried with phosphorus pentoxide or anhydrous calcium chloride, and multiple batches of dried snake venom are mixed according to snake species, and stored in brown bottles for frozen storage.

[0023] Determination of toxicity: The Blliss simplified probability unit method is used to determine the toxicity of snake venom, and it is expressed as the half-dead dose LD50. Randomly select 18-20 grams of mice to form several groups, with more than 6 mice in each group, and adjust the dose after pre-testing. The mortality rate of the highest dose group is close to 100%, the mortality rate of the lowest dose group is 0%, the ratio between doses is con...

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Abstract

The invention discloses a method for refining a polyvalent ASV (anti-snake venom) lyophilized serum, pertaining to an immune serum containing antibody which aims at least two kinds of antigens. The serum takes the venom of Ancistrodon acutus, Agkistrodon halys, Pit-vipers, bamboo snakes, adders and the like as the immune antigen which is inoculated into the bodies of animals after degerming and dialysis, and then titer immune serum after purification is used for collecting the antibody which is then desalted and lyophilized; mice are used for evaluating the LD50 of the virulence of the venom and the titer of the ASV serum; the antibody inoculated into the bodies of the animals is one of the venom, toxoid or snake venom or the composition thereof, and glutaraldehyde or formaldehyde is used for toxin detoxication; before immunity, anti-tetanic toxoid condensed antibody for human beings is used for carrying out minim gradual immunization on the animals, or an Emuade adjuvant is used for immunization purpose. The polyvalent ASV lyophilized serum complies with the biological product standards upon the tests and inspections of purity and specific activity, safety, pyrogen, hypersusceptibility, dissolution and the like; moreover, the serum can cure sufferers who are bitten by vipers with the advantages of high purity, low foreign protein, easy preservation and quick dissolving capacity.

Description

Technical field [0001] The present invention belongs to immune serum containing antibodies, especially immune serum containing antibodies against two or more antigens. Background technique [0002] There are nearly 200 species of snakes in my country, among which there are more than 50 species of venomous snakes, including 16 species of highly venomous sea snakes and nearly 40 species of terrestrial highly venomous and lightly venomous snakes. In my country, there are 9 species of terrestrial venomous snakes and 2 species of sea snakes that are widely distributed and harmful. They are: five-step snake (Agkistrodon acutusGuenther); pit viper (Agkistrodon blomhoffii boie); ironhead (Trimeresurus mucrosquamatusCantor); bamboo leaf green (Trimeresurus stejnegeri Schmidt); viper (Vipera russelli Siamensissmith); Golden krait (Bungarus fasciatus Schneider); Cobra (Na ja na ja atra Cantor); King cobra (Ophiophagus Hannah Cantor); Green-ringed sea snake (Hydrophis Cyanocinctus Daud...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/395A61P17/02
Inventor 范泉水王双印郑颖邱薇杨美峰尹惠琼李作生冯子良
Owner KUNMING INST OF MILITARY MEDICINE CHENGDU MILITARY REGIONS CENT FOR DISEASE CONTROL & PREVENTION
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