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Fingerprint of a Boletus nigricans strain yl1701-2 and its ssr marker

A technology of black boletus and strains, applied in the direction of fungi, fungal products, microorganisms, etc., can solve the problems of irregular fruiting, no fruiting, long fruiting cycle, etc.

Active Publication Date: 2021-08-13
JINGHONG HONGZHEN AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yet the bacterial classification that this method obtains is because of source different, and the character instability often occurs mycelia growth is slow, grows inhomogeneously, the fruiting period is long, the fruiting rate is low, the fruiting is not neat, and the fruiting body output is low (biological efficiency 25%, the average single bottle produces fresh boletus 100g), the output fluctuation is unstable or even no mushrooms, etc.
Because of the instability of the strain, the artificial cultivation of black boletus has remained in the laboratory stage, and it is impossible to carry out large-scale factory cultivation, which has seriously affected the cultivation scale and industrial development of black boletus.

Method used

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  • Fingerprint of a Boletus nigricans strain yl1701-2 and its ssr marker
  • Fingerprint of a Boletus nigricans strain yl1701-2 and its ssr marker
  • Fingerprint of a Boletus nigricans strain yl1701-2 and its ssr marker

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Adopt sexual hybridization breeding selection method to obtain black boletus (Phlebopus portentosus) YL1701-2 bacterial strain of the present invention:

[0042] 1. The parental strains are the production strain HZ14080 and the wild domesticated strain H216009;

[0043] 1.1 Strain HZ14080 has the following characteristics: gray-brown cap and stipe, large cap (diameter: 7-15 mm), cylindrical stipe, small difference between upper and lower diameters (about 10 mm), good mushroom shape, The weight is heavy (average mushroom weight: 90g), the fruiting rate is high (95%-98%), and the output is stable. The disadvantages are: after cooking, the muddy smell is heavy, the browning is serious, and the color of the mushroom body turns dark brown after cooking.

[0044] 1.2 The wild strain H216009 has the following characteristics: the cap and stipe are yellow-brown, the flesh is yellow, the cap is small (diameter: 5-12mm), the upper part of the stipe is thin, the lower part sudden...

Embodiment 2

[0054] The application of strain YL1701-2 in the industrial cultivation of edible fungus: use the strain to carry out artificial cultivation of fruiting bodies to test its excellent characteristics in terms of production speed, uniformity of fruiting, yield, taste, and shelf life.

[0055] The strain YL1701-2 can complete the life cycle from the strain to the basidiocarp on the artificial substrate, that is, the artificial cultivation of the fruiting bodies can be carried out through the strains under artificial conditions, so as to achieve the purpose of harvesting the fruiting bodies of the black boletus. Compared with the existing wild domesticated strains, it has the characteristics of fast growth, fast fruiting, higher yield and longer fresh-keeping period.

[0056] YL1701-2 factory cultivation experiment and production cultivation application. The method of step-by-step expansion cultivation is adopted: 500 bottles for initial test, then 4000 bottles for small test, 8000...

Embodiment 3

[0078] Construct the SSR-marked fingerprint of Boletus nigra YL1701-2 strain:

[0079] Through the development of microsatellite fingerprints, the genetic relationship analysis and species definition of YL1701-2 strain and other black boletus strains were accurately and quickly carried out:

[0080] 1. Mycelia culture: transfer the YL1701-2 strain to PDA medium, and cultivate it at 26°C for 20 days. After the mycelia cover the surface of the medium, collect the mycelium with a scraper and transfer it to a centrifuge tube for use in YL1701 -2 Genomic DNA extraction.

[0081] 2. Extraction and detection of genomic DNA of strain YL1701-2: The total DNA of YL1701-2 was extracted using the Omega mag-bind DNA kit, and the operation method was carried out according to the instructions provided by the kit. The extracted DNA sample was mixed with 10X LoadingBuffer at a ratio of 10:1 and loaded on a 1% agarose gel for electrophoresis, and the quality of the extracted total DNA was dete...

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Abstract

The invention discloses a black boletus strain YL1701-2 and its SSR marker fingerprint. This strain was obtained from Dictyostelium boletus HZ14080 and H216009 through sexual cross breeding. It has the ability of factory cultivation and was deposited in the General Microbiology Center of the China Microbial Culture Collection Committee on February 25, 2019. CGMCC, deposit number CGMCC No.17073. The SSR marker fingerprint of YL1701‑2 strain consists of 12 pairs of SSR markers. The invention realizes the industrial cultivation of black boletus for the first time, and overcomes the problems of existing artificially cultivated strains of black boletus such as unstable properties, slow mycelial growth, long fruiting cycle, low fruiting rate, and low yield. , greatly expanding the scale of cultivation. Currently, 50,000 bottles of black boletus are cultivated daily and 6 tons of fresh black boletus are produced daily. The output and quality are stable. It also solves the problems of wild black porcini mushrooms having a strong muddy smell and being easy to brown after cooking.

Description

technical field [0001] The invention belongs to the technical field of edible fungus artificial breeding and identification of strains, and in particular relates to a new strain of boletus nigra which is obtained through sexual cross breeding, has excellent biological characteristics and can realize industrialized cultivation. At the same time, the invention also relates to a specific SSR marker fingerprint that can accurately identify the new strain of Boletus nigricans. Background technique [0002] Black boletus (Phlebopus portentosus) is a fungus of the genus Phlebopus in the family Boletales, Boletales family Boletales. It is a large and valuable edible fungus distributed in the pantropics. It is also the only one that can completely break away from symbiosis. Or parasitic tree species realizes the boletus species cultivated under artificial conditions, and the market prospect is broad. [0003] Strains are the primary problem to be solved in the artificial cultivation...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01H15/00C12Q1/6895C12R1/645
CPCA01H15/00C12Q1/6895C12Q2600/156C12N1/145C12R2001/645
Inventor 罗顺珍纪开萍纪光燕纪光玉高丽霞范春梦王秋兰陈建华
Owner JINGHONG HONGZHEN AGRI TECH CO LTD
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