A method for artificially cultivating fruiting bodies of Wuling ginseng by using inner cavity solid medium

A solid medium and artificial cultivation technology, which is applied in the field of artificially cultivating fruiting bodies of Wulingshen by using a solid medium in the inner cavity, can solve the problem that the value of Wulingshen is not brought into full play, the purity of fruiting bodies of Wulingshen is poor, and the bacteria High pollution risk and other issues, achieve high medicinal value, short cultivation cycle, and reduce planting costs

Active Publication Date: 2022-04-15
黄美琼
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because Wulingshen grows on the abandoned nests of black-winged soil termites 0.5 to 2 meters underground, natural resources are very scarce, extremely precious in nature, and the output is very small, which leads to most people not knowing Wulingshen, so until now The development and utilization of the efficacy of Wulingshen sclerotium is almost blank, and the value of Wulingshen to human health has not been brought into play
[0004] At present, there are medicines produced by Wulingshen submerged fermentation technology in the pharmaceutical market. There are few reports on the cultivation methods of artificial fruiting bodies of Wulingshen that can be industrialized, and most of them use liquid strains, which have high requirements for equipment and risk of strain contamination. High, once the pollution leads to the failure of the whole batch, and it has the disadvantages of inconvenient transportation and storage resistance
ZL201410242158.X discloses a method for simultaneously obtaining selenium-enriched solid fermentation mycelia and selenium-enriched Wulingshen fruiting bodies, but the production of the medium is complicated and the yield is low
ZL201910073968.X discloses a solid medium for the fruiting body of Wulingshen and its artificial cultivation method. The mycelia culture time is too long; The purity of Lingshen fruiting body is poor
ZL201911125279.5 also discloses an artificial cultivation method of Wuling ginseng, but the cultivation period is too long

Method used

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  • A method for artificially cultivating fruiting bodies of Wuling ginseng by using inner cavity solid medium
  • A method for artificially cultivating fruiting bodies of Wuling ginseng by using inner cavity solid medium
  • A method for artificially cultivating fruiting bodies of Wuling ginseng by using inner cavity solid medium

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Embodiment 1

[0059] A method for artificially cultivating fruiting bodies of Wuling ginseng by using a solid medium in an inner cavity, characterized in that it comprises the following steps:

[0060] Step (1), preparation of Wuling ginseng mother species: under sterile conditions, inoculate the spores collected from Wuling ginseng on the slant of the mother seed culture medium, and cultivate in the dark at 22°C-26°C for 8 days to obtain first generation mother species;

[0061] Then, under aseptic conditions, inoculate the non-polluted and robust mycelium of Wulingshen from the obtained first generation mother species to the slant of the new mother species culture medium, and culture in dark at 22°C-26°C for 6.5 days to obtain the purified mother species;

[0062] The mother seed medium includes the following raw materials in parts by weight: 200 parts of peeled potatoes, 20 parts of glucose, 20 parts of agar powder, 2 parts of peptone, 2 parts of potassium dihydrogen phosphate, 1.5 parts...

Embodiment 2

[0069] A method for artificially cultivating fruiting bodies of Wuling ginseng by using a solid medium in an inner cavity, characterized in that it comprises the following steps:

[0070] Step (1), preparation of Wuling ginseng mother species: under sterile conditions, inoculate the spores collected from Wuling ginseng on the slant of the mother seed culture medium, and cultivate in dark at 22°C-24°C for 7 days to obtain first generation mother species;

[0071] Then, under aseptic conditions, inoculate the non-polluted and robust mycelia of Wulingshen from the obtained first-generation mother species to the slant of the new mother species culture medium, and culture in dark at 22°C-24°C for 5 days to obtain the purified mother species;

[0072] The mother seed medium includes the following raw materials in parts by weight: 150 parts of peeled potatoes, 15 parts of glucose, 15 parts of agar powder, 1 part of peptone, 1 part of potassium dihydrogen phosphate, 1 part of magnesiu...

Embodiment 3

[0095] A method for artificially cultivating fruiting bodies of Wuling ginseng by using a solid medium in an inner cavity, characterized in that it comprises the following steps:

[0096] Step (1), preparation of Wuling ginseng mother species: under sterile conditions, inoculate the spores collected from Wuling ginseng on the slant of the mother seed culture medium, and culture in dark at 24°C-26°C for 10 days to obtain first generation mother species;

[0097] Then, under aseptic conditions, inoculate the non-polluted and robust mycelium of Wulingshen from the obtained first generation mother species to the slant of the new mother species culture medium, and cultivate in dark at 25°C-26°C for 7 days to obtain the purified mother species;

[0098] The mother seed medium includes the following raw materials in parts by weight: 250 parts of peeled potatoes, 25 parts of glucose, 25 parts of agar powder, 3 parts of peptone, 3 parts of potassium dihydrogen phosphate, 2 parts of mag...

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Abstract

The invention relates to a method for artificially cultivating fruiting bodies of Wulingshen by using a solid medium in an inner cavity, and belongs to the technical field of artificial cultivation of fruiting bodies of Wulingshen. The method comprises several major steps of preparing the female seed of Wulingshen, preparing the original seed of Wulingshen, culturing the mycelium of Wulingshen, and cultivating fruiting bodies of Wulingshen. The method of the invention is simple to operate, the growth of the fruit body of Wulingshen simulates the natural cavity environment, the fruit body grows rapidly, and the cultivation period is short. The air in the inner cavity of the medium is filtered through a sterile breathable membrane, which is very clean, and at the same time increases the carbon dioxide concentration in the inner cavity, which is more conducive to the growth of the fruiting body of Wulingshen. The fruiting body of Wulingshen grows in a pollution-free environment. The young and fresh fruiting body of Wulingshen can be eaten raw directly, with a biotransformation rate of 30-50%, no environmental pollution, no waste, industrialized production, easy to popularize and apply .

Description

technical field [0001] The invention belongs to the technical field of artificial cultivation of fruiting bodies of Wulingshen, and in particular relates to a method for artificially cultivating fruiting bodies of Wulingshen by using a solid medium in an inner cavity. Background technique [0002] Wulingshen is also known as Wulingshen, Wulishen, Chicken Poria, Jicong Gallbladder, Chicken Congdan, Diaojinzhong, Lei Zhenzi, etc. It is a waste soil-dwelling termite that grows at a depth of 2-50 meters underground. The rare medicinal fungus in the nest is the sclerotia formed by the mycelium of C. nigriscens, which is a precious traditional Chinese medicine. In terms of classification, the sclerotium of Xylaria nigripes (Kl .) Sacc.] belongs to Eumycota, Ascomycotina, Xylariaceae, Xylaria ( Xylaria) (Mao Xiaolan's "Chinese Macrofungi"). Wuling ginseng is distributed in Yunnan, Sichuan, Jiangxi, Hunan, Fujian and other provinces. It is spherical, ellipsoidal or ovoid to irregu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/20A01G18/00A01G18/40A01G18/50
CPCA01G18/20A01G18/00A01G18/40A01G18/50
Inventor 黄美琼
Owner 黄美琼
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