Industrialized tissue culture and rapid propagation method for astragalus membranaceus

Abstract

The invention provides an industrialized tissue culture and rapid propagation method for astragalus membranacus, and relates to the technical field of tissue culture and rapid propagation of traditional Chinese medicines. Through callus induction, callus proliferation and differentiation culture and rooting culture, a large number of pure astragalus membranacus seedlings can be obtained all the year round. The proliferation coefficient of the astragalus membranaceus cluster buds obtained by the industrialized tissue culture and rapid propagation method reaches 13-15 times, the cluster buds are robust, the cluster buds are easy to root after being inoculated to an open rooting culture medium, the rooting rate reaches 90% or above, the later transplanting survival rate is 90% or above, and high-quality astragalus membranaceus seedlings are obtained. The industrialized tissue culture and rapid propagation method for astragalus membranacus is not limited by time and space, factory cultivation can be achieved all the year round, and production requirements are met.

Description

technical field [0001] The invention belongs to the technical field of tissue culture and rapid propagation of traditional Chinese medicine, and in particular relates to a method for industrialized tissue culture and rapid propagation of Astragalus membranaceus. Background technique [0002] Astragalus membrane pod (Astragalus membranaceus (fisch.) Bunge) is a perennial herbaceous plant of the leguminous family, a national third-level protected plant, and is a genuine product of Astragalus membranaceus in the Pharmacopoeia. It is produced in Heilongjiang, Jilin, Liaoning, Inner Mongolia, Hebei, Shanxi, Shaanxi, Ningxia, Gansu , Qinghai, Xinjiang, Shandong and Sichuan. It grows on the edge of the forest or under the shrubs and sparse forests. ) and Huayuan (Yao County, Shaanxi) are also good." The Liupanshan area of ​​Ningxia is the main producing area of ​​"Yuanzhou Astragalus", and the local people have a long history of artificial domestication and cultivation of wild Ast...

AI Technical Summary

Problems solved by technology

Astragalus membranaceus is the top grade of Astragalus membranaceus, which has the functions of invigorating qi and raising yang, strengthening the surface and antiperspirant, diuresis and detumescence; it is a traditional Chinese medicinal material commonly used by doctors in the past dynasties, and the market demand is huge. It is on the verge of extinction. At present, the artificially cultivated Astragalus membranaceus is mostly mixed with Astragalus membranaceus, and it is difficult to obtain pure Astragalus membranaceus seedlings. Therefore, it is necessary to cultivate non-separating traits and consistent growth through tissue culture rapid propagation and industrial seedling technology. , Pure quality Astragalus membranaceus seedlings

At present, there are no reports on tissue culture, rapid propagation and industrial seedling cultivation of Astragalus membranaceus

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