Rapid propagation method of wild betula platyphylla suk

A birch, wild technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as difficulty in meeting market demand, slow reproduction speed, etc., to achieve strong adaptability to urban environments, uniform seedlings, and disease resistance. The powerful effect of insects

Active Publication Date: 2016-12-07
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, white birch has been listed as a national second-class protected plant and a key plant protection resource. Moreover, white birch mainly reproduces by seeds and the reproduction speed is slow, which is difficult to meet market demand.

Method used

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  • Rapid propagation method of wild betula platyphylla suk
  • Rapid propagation method of wild betula platyphylla suk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, a kind of rapid propagation method of wild white birch

[0030] (1) The method is as follows:

[0031] 1) Obtaining of wild white birch stem section explants:

[0032] Cutting out 6 cm long stems containing buds from wild white birch adult trees is explants, after the explants are sterilized, rinsed with sterile water, and sucked dry, the branches are cut into stems with an axillary bud;

[0033] 2) Induction culture of wild white birch stem section explants:

[0034] The obtained explants are induced and cultivated at a temperature of 25-28°C and a light intensity of 2200-2500 Lux, with light for 14-16 hours per day, and after 40-45 days, the axillary buds begin to germinate and form small buds; the composition of the induction medium is : WPM+6-BA1.5~2.0mg / L+NAA 1.0~2.0mg / L+sucrose 40g / L+agar 5g / L, pH5.5~6.5;

[0035] 3) Proliferation and cultivation of wild white birch cluster buds:

[0036] Cut the cultivated new long axillary bud seedlings into st...

Embodiment 2

[0044] Embodiment 2 comparative experiment

[0045] 1. Material selection:

[0046]Cut 6cm-long stems containing buds from 8-10-year-old wild white birch trees as explants, disinfect the explants, wash them with sterile water, and absorb the water, then cut the branches with an axillary bud 80 stem segments were randomly divided into 4 groups.

[0047] Group 1: adopt the method of Example 1.

[0048] Group 2: the same method as in Example 1, the difference is: in the 2nd) step, the rapid propagation method of wild white birch of the present invention is not adopted, but MS+6-BA 0.5~1.0mg / L+NAA 0.5~ 1.0mg / L+ sucrose 40g / L+ agar 5g / L, pH5.5~6.5;

[0049] Group 3: the same method as in Example 1, the difference is: in the 3rd) step, instead of using the rapid propagation method of wild white birch of the present invention, MS+NAA1.0~2.0mg / L+IBA0.05~ 0.2mg / L+ sucrose 40g / L+ agar 5g / L, pH5.5~6.5;

[0050] Group 4: the same method as Example 1, the difference is: in the 4th) st...

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Abstract

The invention provides a rapid propagation method of wild betula platyphylla suk. The rapid propagation method comprises the following steps: 1) obtainment of the stem explant of the wild betula platyphylla suk; 2) inducing culture of the stem explant of the wild betula platyphylla suk; 3) enrichment culture of clustered shoots of the wild betula platyphylla suk; 4) rooting culture of the wild betula platyphylla suk: at the first stage, firstly culturing single shoots cut from the clustered shoots in a first rooting culture medium, putting the single shoots under 2800-3000Lux and illuminating the single shoots for 20 hours everyday to culture the single shoots until the single shoots root for 8-10 days; at the second stage, transferring the single shoots to a second rooting culture medium to be cultured for 20-30 days until good root systems grow; 5) hardening and transplanting of wild betula platyphylla suk tissue culture seedlings: carrying out hardening under normal temperature for 7-10 days and then transplanting the seedlings to a substrate to be cultured for 30-35 days, thus obtaining rapid propagation seedlings of the wild betula platyphylla suk; 6) transplanting: transplanting the seedlings to planting sites. The problems that the wild betula platyphylla suk is difficult in tissue culture and rooting and has low rooting rate are solved by adopting the rapid propagation method.

Description

technical field [0001] The invention relates to a rapid propagation method of wild white birch. Background technique [0002] White birch (Bettlla platyphylla suk), belonging to Betaceae Birch, deciduous tree, up to 20-25m in height, 50cm in diameter at breast height, white, smooth, papery layered peeling off; twigs brown reddish brown, leaves triangular oval , apex acuminate, base truncated, nutlets flat, membranous wings on both sides, flowering in May-June, fruit ripening in August. [0003] White birch is produced in the Greater Khingan Mountains, the Lesser Khingan Mountains, the Changbai Mountains in Northeast my country, the Altai Mountains in the North China and the Altai Mountains in the Northwest; it is distributed in the Greater Khingan Mountains, the Lesser Khingan Mountains, and the Changbai Mountains in Northeast China, Shanxi, Hunan, and Hubei in North China, and Inner Mongolia, Ningxia, Shaanxi, Qinghai, and Tibet in the Northwest. , Sichuan in the southwest,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李悦陈忠林张晋李丽丹艾娇张壮
Owner LIAONING UNIVERSITY
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