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A kind of factory tissue culture fast propagation method of Astragalus membranaceus

A technology of tissue culture rapid propagation and Astragalus membranaceus, applied in horticultural methods, botanical equipment and methods, climate change adaptation, etc., can solve the problems of endangered and difficult to obtain Astragalus membranaceus seedlings, etc., to meet production needs Effect

Active Publication Date: 2022-07-08
宁夏农林科学院农业生物技术研究中心
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AI Technical Summary

Problems solved by technology

Astragalus membranaceus is the top grade of Astragalus membranaceus, which has the functions of invigorating qi and raising yang, strengthening the surface and antiperspirant, diuresis and detumescence; it is a traditional Chinese medicinal material commonly used by doctors in the past dynasties, and the market demand is huge. It is on the verge of extinction. At present, the artificially cultivated Astragalus membranaceus is mostly mixed with Astragalus membranaceus, and it is difficult to obtain pure Astragalus membranaceus seedlings. Therefore, it is necessary to cultivate non-separating traits and consistent growth through tissue culture rapid propagation and industrial seedling technology. , Pure quality Astragalus membranaceus seedlings
At present, there are no reports on tissue culture, rapid propagation and industrial seedling cultivation of Astragalus membranaceus

Method used

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  • A kind of factory tissue culture fast propagation method of Astragalus membranaceus
  • A kind of factory tissue culture fast propagation method of Astragalus membranaceus
  • A kind of factory tissue culture fast propagation method of Astragalus membranaceus

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Embodiment 1

[0034] 1. Obtaining test-tube seedlings of Astragalus membranaceus, the specific steps are as follows:

[0035] 1. Explant selection and processing

[0036] In late May, the wild Astragalus membranaceus has just grown new branches of 10-15cm, select a strong and good single plant, cut the new shoots, wrap it with wet newspaper, put it into a sampling refrigerator and incubator, and bring it back to the tissue culture as soon as possible. room for pretreatment.

[0037] Take out the twigs of Astragalus membranaceus brought back, rinse the surface with running water for 0.5h, wash the twigs and leaves with a soft brush dipped in detergent water, rinse with clean water, and divide into 4-5cm small sections with leaves.

[0038] 2. Sterilization of explants:

[0039] In the ultra-clean workbench, put the segmented explants into sterile glass bottles with lids, add 2% sodium hypochlorite to submerge the explants, drop 1-2 drops of Tween 20, and shake them by hand. Glass containe...

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Abstract

The invention provides an industrial tissue culture rapid propagation method of Astragalus pods, and relates to the technical field of tissue culture rapid propagation of traditional Chinese medicines. The invention obtains a large number of pure Astragalus membranaceus seedlings every year by inducing callus, callus proliferation and differentiation culture and rooting culture. The proliferation coefficient of Astragalus membranaceus cluster buds obtained by the tissue culture rapid propagation method of the present invention reaches 13 to 15 times, the cluster buds are robust, easy to take root when inoculated on an open rooting medium, and the rooting rate reaches more than 90%, and the later stage The survival rate of transplanting is over 90%, and the seedlings of Astragalus membranaceus are obtained. The invention is not limited by time and space, can realize factory cultivation every year, and can meet production needs.

Description

technical field [0001] The invention belongs to the technical field of tissue culture rapid propagation of traditional Chinese medicines, in particular to an industrialized tissue culture rapid propagation method of Astragalus membranaceus. Background technique [0002] Astragalus membranaceus (fisch.) Bunge is a leguminous perennial herb, a national level 3 protected plant, and the authentic product of Pharmacopoeia Astragalus, produced in Heilongjiang, Jilin, Liaoning, Inner Mongolia, Hebei, Shanxi, Shaanxi, Ningxia, Gansu , Qinghai, Xinjiang, Shandong and Sichuan, growing in forest margins or under thickets and sparse forests, astragalus formerly known as Astragalus, was first recorded in "Shen Nong's Materia Medica", Song "Map of Materia Medica" contains: "now out of Yuanzhou (Ningxia Guyuan) ) and Huayuan (Yao County, Shaanxi Province) are also good”. The Liupanshan area of ​​Ningxia is the main producing area of ​​"Yuanzhou Astragalus", and the local people have a lon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008Y02P60/40
Inventor 郭生虎陈虞超李明朱永兴关雅静张茜石磊刘华
Owner 宁夏农林科学院农业生物技术研究中心
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