A kind of liquid culture method and cultivation method of black boletus

A technology of black boletus and liquid strains, which is applied in cultivation, plant cultivation, mushroom cultivation, etc. It can solve the problems of bacteria cultivation period pollution, long cultivation period, and small number of natural picking, so as to improve efficiency and increase growth rate Effect

Active Publication Date: 2021-03-05
JINGHONG HONGZHEN AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Black boletus Phlebopus portentosus (Berk&Broome) Boedijn, also known as dark reticulate boletus, belongs to Boletinellaceae (Boletinellaceae), delicious taste, rich nutrition, its water extract has anticancer and antiviral properties Function, and rich in mineral elements and a variety of essential amino acids for the human body, it is deeply loved by consumers because of its edible and medicinal value. The number of natural picking is very small, which cannot meet the market demand. The existing technology has been completed Black liver fungus factory cultivation technology model, and factory cultivation is an inevitable trend
However, the cultivation period of the solid strain of Boletus nigra is long, and it is difficult to control a large amount of pollution during the cultivation period due to the invisible pollution of the solid strain.
At present, the liquid strains of boletus are mainly cultivated in Erlenmeyer flask shake flasks. The efficiency of obtaining liquid strains of bacteria in Erlenmeyer flask shake flasks is low, and mechanical inoculation cannot be performed. At present, manual inoculation is relied on, which is not suitable for industrial scale cultivation.
[0003] Fermentation tank culture technology has the advantages of short production cycle, consistent bacterial age, low strain cost, etc., and can be mechanically inoculated. However, there are few reports on the fermentation tank culture technology of black boletus

Method used

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  • A kind of liquid culture method and cultivation method of black boletus
  • A kind of liquid culture method and cultivation method of black boletus
  • A kind of liquid culture method and cultivation method of black boletus

Examples

Experimental program
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Effect test

Embodiment 1

[0057] 1. Mother species activation

[0058] Mother species activation medium formula (referred to as M1):

[0059]

[0060] M1 preparation: Accurately weigh the above-mentioned medicines, wash and peel the potatoes, cut them into thin slices, weigh the potatoes according to the required amount, take a clean iron basin and add 200mL more water to the required nutrient solution, put the weighed potatoes, heat to boil until When the potatoes become soft, filter the potato boiled juice with 4 layers of gauze, dissolve the drug in the filtered potato boiled juice, divide it into test tubes at a fixed volume, sterilize at 115°C for 15 minutes, put it on a slope, and cool it for later use.

[0061] parent species activation

[0062] Inoculate the preserved mother species in the above-mentioned (2) test tube slant medium, culture at 28°C for 30 days, the hyphae will cover the slant, and the activation of the mother species will be completed.

[0063] Second, the first-level liqu...

Embodiment 2-3

[0089] Bacterial strain activation, one-level liquid seed cultivation, liquid strain fermentation culture method and fruiting verification are identical with embodiment 1, difference is: the fermentor volume 750L / tank of embodiment 2, the fermenter volume 1000L / jar of embodiment 3 Can. After the culture of the strain in the fermenter is completed, the machine is used for inoculation.

Embodiment 4

[0091] Strain activation, first-level liquid seed cultivation, liquid strain fermentation culture method and fruiting verification are the same as in Example 1, except that the liquid strain fermentation conditions are as follows:

[0092] (1) The hyphae of the strain (ball) are broken to increase the germination point: adjust the culture temperature to 16°C, the ventilation pressure to 0.025Mpa, stop the ventilation after 0.75h of ventilation, adjust the motor speed to 5000ramp / min, and cultivate for 2.5h.

[0093] (2) Mycelia recovery: After the mycelium is broken, adjust the appropriate medium temperature (23°C), medium speed (550ramp / min) and ventilation pressure of 0.04Mpa, and cultivate for 10h to allow the mycelia to recover.

[0094] (3) Cultivate to adapt to germination: adjust the temperature to 29.5°C, the ventilation pressure to 0.06Mpa, the rotation speed to 475 / min, and cultivate for 23 hours to allow the hyphae to germinate;

[0095] (4) Mycelia growth: adjust t...

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Abstract

The invention relates to a method for cultivating liquid strains of black boletus and a cultivation method. A method for cultivating liquid strains of black boletus, comprising the following steps: inoculating the liquid strains of black boletus in a fermenter, undergoing broken mycelia of the strain, recovery of mycelium, cultivation to adapt to germination, and growth of mycelia , mycelia multiple growth period, and inoculation cultivation before the cultivation of the six stages of the adaptation period, and then complete the fermentation; the present invention has formulated different culture conditions for the growth characteristics of bacteria in different stages, so that the black boletus is suitable for fermentation in the fermenter grow and achieve a higher growth rate. The present invention explores the cultivation conditions of the black boletus liquid strain in the fermenter, provides the feasibility for mechanized inoculation, has higher production efficiency than the existing triangular flask cultivation method, and has a higher pollution rate than the solid strain cultivation method Low.

Description

technical field [0001] The invention relates to the technical field of edible fungus cultivation, in particular to a liquid strain cultivation method and cultivation method of black boletus. Background technique [0002] Black boletus Phlebopus portentosus (Berk&Broome) Boedijn, also known as dark reticulate boletus, belongs to Boletinellaceae (Boletinellaceae), delicious taste, rich nutrition, its water extract has anticancer and antiviral properties Function, and rich in mineral elements and a variety of essential amino acids for the human body, it is deeply loved by consumers because of its edible and medicinal value. The number of natural picking is very small, which cannot meet the market demand. The existing technology has been completed Black liver fungus factory cultivation technology model, and factory cultivation is an inevitable trend. However, the cultivation period of the solid strain of Boletus nigra is long, and it is difficult to control the large amount of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/40A01G18/20
CPCA01G18/20A01G18/40
Inventor 魏学林张倩李春会高丽霞罗顺珍纪光燕纪光玉玉应香
Owner JINGHONG HONGZHEN AGRI TECH CO LTD
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